scholarly journals The Magnetic Receptor of Monascus ruber M7: Gene Clone and Its Heterologous Expression in Escherichia coli

2020 ◽  
Vol 11 ◽  
Author(s):  
Hongyi Zhou ◽  
Shuyan Yang ◽  
Fusheng Chen
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Gene Clone ◽  
Monascus Ruber ◽  
Expression In Escherichia Coli

Mining Xanthomonas and Streptomyces genomes for new pectinase-encoding sequences and their heterologous expression in Escherichia coli

2008 ◽  
Vol 78 (6) ◽  
pp. 973-981 ◽  
Author(s):  
Zhizhuang Xiao ◽  
Jason Boyd ◽  
Stephan Grosse ◽  
Manon Beauchemin ◽  
Elizabeth Coupe ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Expression In Escherichia Coli

The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli

2012 ◽  
Vol 40 (6) ◽  
pp. 1222-1226 ◽  
Author(s):  
Katrin Richter ◽  
Johannes Gescher
Keyword(s):  
Applied Sciences ◽  
Escherichia Coli ◽  
Synthetic Biology ◽  
Heterologous Expression ◽  
Basic Science ◽  
Gene Dosage ◽  
Toxic Effects ◽  
Genomic Expression ◽  
Expression In Escherichia Coli ◽  
Promising Tool

Heterologous multiprotein expression is the tool to answer a number of questions in basic science as well as to convert strains into producers and/or consumers of certain compounds in applied sciences. Multiprotein expression can be driven by plasmids with the disadvantages that the gene dosage might, in some cases, lead to toxic effects and that the continuous addition of antibiotics is undesirable. Stable genomic expression of proteins can forgo these problems and is a helpful and promising tool in synthetic biology. In the present paper, we provide an extract of methods from the toolbox for chromosome-based heterologous expression in Escherichia coli.

Uncoupling protein, H+ transport and regulation

2001 ◽  
Vol 29 (6) ◽  
pp. 806-811 ◽  
Author(s):  
M. Klingenberg ◽  
E. Winkler ◽  
K. Echtay
Keyword(s):  
Escherichia Coli ◽  
Fatty Acids ◽  
Heterologous Expression ◽  
Inclusion Bodies ◽  
Uncoupling Protein ◽  
Coenzyme Q ◽  
Uncoupling Proteins ◽  
Expression In Escherichia Coli ◽  
Cl Transport

The biochemical functions of uncoupling proteins (UCPs) are discussed with the view of UCP1 as a paradigm. In contrast with UCP1, the heterologous expression of UCP3 in yeast is found to result primarily in extra-mitochondrial deposits and thus is unsuitable for studying UCP3 function. On expression in Escherichia coli inclusion bodies, UCPs extracted and incorporated into vesicles showed no H+ transport, only Cl– transport. Only after addition of coenzyme Q was fully nucleotide-sensitive high-H+ transport reconstituted, with UCP1 as well as with UCP2 and UCP3. The newly discovered cofactor role of coenzyme Q in H+ transport is proposed to imply co-operation with fatty acids for the injection of H+ into the UCP channel.

scholarly journals ccSOL omics: a webserver for solubility prediction of endogenous and heterologous expression in Escherichia coli

2014 ◽  
Vol 30 (20) ◽  
pp. 2975-2977 ◽  
Author(s):  
F. Agostini ◽  
D. Cirillo ◽  
C. M. Livi ◽  
R. Delli Ponti ◽  
G. G. Tartaglia
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Solubility Prediction ◽  
Expression In Escherichia Coli
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