Pinus taeda phenylpropenal double-bond reductase: Purification, cDNA cloning, heterologous expression in Escherichia coli, and subcellular localization in P. taeda☆

2006 ◽  
Vol 67 (16) ◽  
pp. 1765-1780 ◽  
Author(s):  
Hiroyuki Kasahara ◽  
Ying Jiao ◽  
Diana L. Bedgar ◽  
Sung-Jin Kim ◽  
Ann M. Patten ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Double Bond ◽  
Subcellular Localization ◽  
Heterologous Expression ◽  
Pinus Taeda ◽  
Cdna Cloning ◽  
Expression In Escherichia Coli ◽  
Double Bond Reductase

Mining Xanthomonas and Streptomyces genomes for new pectinase-encoding sequences and their heterologous expression in Escherichia coli

2008 ◽  
Vol 78 (6) ◽  
pp. 973-981 ◽  
Author(s):  
Zhizhuang Xiao ◽  
Jason Boyd ◽  
Stephan Grosse ◽  
Manon Beauchemin ◽  
Elizabeth Coupe ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Expression In Escherichia Coli

cDNA cloning of a novel gene codifying for the enzyme lycopene β-cyclase from Ficus carica and its expression in Escherichia coli

2011 ◽  
Vol 92 (4) ◽  
pp. 769-777 ◽  
Author(s):  
José Miguel Araya-Garay ◽  
Lucía Feijoo-Siota ◽  
Patricia Veiga-Crespo ◽  
Tomás González Villa
Keyword(s):  
Escherichia Coli ◽  
Cdna Cloning ◽  
Ficus Carica ◽  
Expression In Escherichia Coli ◽  
Novel Gene

The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli

2012 ◽  
Vol 40 (6) ◽  
pp. 1222-1226 ◽  
Author(s):  
Katrin Richter ◽  
Johannes Gescher
Keyword(s):  
Applied Sciences ◽  
Escherichia Coli ◽  
Synthetic Biology ◽  
Heterologous Expression ◽  
Basic Science ◽  
Gene Dosage ◽  
Toxic Effects ◽  
Genomic Expression ◽  
Expression In Escherichia Coli ◽  
Promising Tool

Heterologous multiprotein expression is the tool to answer a number of questions in basic science as well as to convert strains into producers and/or consumers of certain compounds in applied sciences. Multiprotein expression can be driven by plasmids with the disadvantages that the gene dosage might, in some cases, lead to toxic effects and that the continuous addition of antibiotics is undesirable. Stable genomic expression of proteins can forgo these problems and is a helpful and promising tool in synthetic biology. In the present paper, we provide an extract of methods from the toolbox for chromosome-based heterologous expression in Escherichia coli.

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