scholarly journals A Novel Antimicrobial Peptide Sparamosin26–54 From the Mud Crab Scylla paramamosain Showing Potent Antifungal Activity Against Cryptococcus neoformans

2021 ◽  
Vol 12 ◽  
Author(s):  
Yan-Chao Chen ◽  
Ying Yang ◽  
Chang Zhang ◽  
Hui-Yun Chen ◽  
Fangyi Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Cell Wall ◽  
Cell Membrane ◽  
Antimicrobial Peptide ◽  
Cryptococcus Neoformans ◽  
Antifungal Drugs ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Mature Peptide

Due to the increasing prevalence of drug-resistant fungi and the limitations of current treatment strategies to fungal infections, exploration and development of new antifungal drugs or substituents are necessary. In the study, a novel antimicrobial peptide, named Sparamosin, was identified in the mud crab Scylla paramamosain, which contains a signal peptide of 22 amino acids and a mature peptide of 54 amino acids. The antimicrobial activity of its synthetic mature peptide and two truncated peptides (Sparamosin1–25 and Sparamosin26–54) were determined. The results showed that Sparamosin26–54 had the strongest activity against a variety of Gram-negative bacteria, Gram-positive bacteria and fungi, in particular had rapid fungicidal kinetics (killed 99% Cryptococcus neoformans within 10 min) and had potent anti-biofilm activity against C. neoformans, but had no cytotoxic effect on mammalian cells. The RNA-seq results showed that after Sparamosin26–54 treatment, the expression of genes involved in cell wall component biosynthesis, cell wall integrity signaling pathway, anti-oxidative stress, apoptosis and DNA repair were significantly up-regulated, indicating that Sparamosin26–54 might disrupt the cell wall of C. neoformans, causing oxidative stress, DNA damage and cell apoptosis. The underlying mechanism was further confirmed. Sparamosin26–54 could bind to several phospholipids in the cell membrane and effectively killed C. neoformans through disrupting the integrity of the cell wall and cell membrane observed by electron microscope and staining assay. In addition, it was found that the accumulation of reactive oxygen species (ROS) increased, the mitochondrial membrane potential (MMP) was disrupted, and DNA fragmentation was induced after Sparamosin26–54 treatment, which are all hallmarks of apoptosis. Taken together, Sparamosin26–54 has a good application prospect as an effective antimicrobial agent, especially for C. neoformans infections.

scholarly journals Antimicrobial Peptide AMP-17 Affects Candida albicans by Disrupting Its Cell Wall and Cell Membrane Integrity

2020 ◽  
Vol Volume 13 ◽  
pp. 2509-2520
Author(s):  
Huiling Ma ◽  
Xinyu Zhao ◽  
Longbing Yang ◽  
Peipei Su ◽  
Ping Fu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Cell Membrane ◽  
Antimicrobial Peptide ◽  
Membrane Integrity ◽  
Cell Membrane Integrity

scholarly journals A Novel Antimicrobial Peptide Scyreprocin From Mud Crab Scylla paramamosain Showing Potent Antifungal and Anti-biofilm Activity

2020 ◽  
Vol 11 ◽  
Author(s):  
Ying Yang ◽  
Fangyi Chen ◽  
Hui-Yun Chen ◽  
Hui Peng ◽  
Hua Hao ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Scylla Paramamosain ◽  
Mud Crab

Characterization and antimicrobial evaluation of Sp PR-AMP1, a proline-rich antimicrobial peptide from the mud crab Scylla paramamosain

2017 ◽  
Vol 74 ◽  
pp. 209-216 ◽  
Author(s):  
Chanprapa Imjongjirak ◽  
Pawanrat Amphaiphan ◽  
Walaiporn Charoensapsri ◽  
Piti Amparyup
Keyword(s):  
Antimicrobial Peptide ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Antimicrobial Evaluation

scholarly journals The interaction between Carbohydrates and the Antimicrobial Peptide P-113Tri is Involved in the Killing of Candida albicans

2020 ◽  
Vol 8 (2) ◽  
pp. 299 ◽  
Author(s):  
Guan-Yu Lin ◽  
Chuan-Fa Chang ◽  
Chung-Yu Lan
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Antimicrobial Peptide ◽  
Mammalian Cells ◽  
Peptide Binding ◽  
Antifungal Drugs ◽  
Planktonic Cells ◽  
Cell Wall Polysaccharides ◽  
The Difference ◽  
Competition Assays

The emergence of drug resistance to Candida albicans is problematic in the clinical setting. Therefore, developing new antifungal drugs is in high demand. Our previous work indicated that the antimicrobial peptide P-113Tri exhibited higher antifungal activity against planktonic cells, biofilm cells, and clinical isolates of Candida species compared to its parental peptide P-113. In this study, we further investigated the difference between these two peptides in their mechanisms against C. albicans. Microscopic examination showed that P-113 rapidly gained access to C. albicans cells. However, most of the P-113Tri remained on the cell surface. Moreover, using a range of cell wall-defective mutants and competition assays, the results indicated that phosphomannan and N-linked mannan in the cell wall are important for peptide binding to C. albicans cells. Furthermore, the addition of exogenous phosphosugars reduced the efficacy of the peptide, suggesting that negatively charged phosphosugars also contributed to the peptide binding to the cell wall polysaccharides. Finally, using a glycan array, P-113Tri, but not P-113, can bind to other glycans commonly present on other microbial and mammalian cells. Together, these results suggest that P-113 and P-113Tri have fundamental differences in their interaction with C. albicans and candidacidal activities.

Hypoxia-induced oxidative stress and transcriptome changes in the mud crab (Scylla paramamosain)

2021 ◽  
Vol 245 ◽  
pp. 109039
Author(s):  
Yu-Kun Jie ◽  
Chang-Hong Cheng ◽  
Li-Cang Wang ◽  
Hong-Ling Ma ◽  
Yi-Qin Deng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Scylla Paramamosain ◽  
Mud Crab

Oxidative stress, cell cycle arrest, DNA damage and apoptosis in the mud crab (Scylla paramamosain) induced by cadmium exposure

Chemosphere ◽  
2021 ◽  
Vol 263 ◽  
pp. 128277
Author(s):  
Chang-Hong Cheng ◽  
Hong-Ling Ma ◽  
Yi-Qin Deng ◽  
Juan Feng ◽  
Yu-Kun Jie ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Dna Damage ◽  
Cell Cycle Arrest ◽  
Cadmium Exposure ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
Cycle Arrest

scholarly journals Effect of Allium cepa on LAC1 gene expression and physiological activities in Cryptococcus neoformans

2021 ◽  
Author(s):  
Seyed Afzal Musavinasab-Mobarakeh ◽  
Masoomeh Shams-Ghahfarokhi ◽  
Mehdi Razzaghi-Abyaneh
Keyword(s):  
Gene Expression ◽  
Cell Membrane ◽  
Cryptococcus Neoformans ◽  
Allium Cepa ◽  
Laccase Activity ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antifungal Drugs ◽  
Melanin Production ◽  
Ergosterol Content

Background and Purpose: This study aimed to investigate the effects of Allium cepa ethanolic extract (EAC) on Cryptococcus neoformans biological activities and LAC1 gene expression. Materials and Methods: The minimum inhibitory concentration (MIC) of EAC was determined based on the Clinical and Laboratory Standards Institute M27-A4 method at a concentration range of 125- 4000 µg/ml. The EAC synergism activity was determined in combination with fluconazole (FCZ) as an antifungal azole. Laccase activity, melanin production, and cell membrane ergosterol content of C. neoformans were assessed at the 0.5× MIC concentration of EAC (1000 μg/ml) and FCZ (64μg/ml) by approved methods. The expression of the LAC1 gene was studied in the fungus exposed to 0.5× MIC concentration of EAC and FCZ using the real-time polymerase chain reaction. Results: Based on obtained results, MIC of EAC and FCZ were 2000 and 128 μg/ml,respectively. A combinatory effect was reported for FCZ and EAC by a fractional inhibitory concentration index of 0.25. The cell membrane ergosterol content was inhibited in EAC- and FCZ-treated C. neoformans by 58.25% and 49.85%, respectively.The laccase activity and melanin production were reduced in EAC-treated C. neoformans by 45.37% and 51.57%, and in FCZ-treated fungus by 54.64% and 53.68%, respectively.The expression of fungal LAC1 at messenger RNA (mRNA) level was measured 0.46 and 0.58 folds and significantly decreased in both EAC- and FCZ-treated C. neoformans at the 0.5×MIC concentration, respectively (p <0.05). Conclusion: The findings revealed that EAC contains inhibitory compounds which interact with biological activities in C. neoformans and thereby, it could be considered as a potential source for the development of novel antifungal drugs.

Effect of nitrite exposure on oxidative stress, DNA damage and apoptosis in mud crab (Scylla paramamosain)

Chemosphere ◽  
2020 ◽  
Vol 239 ◽  
pp. 124668 ◽  
Author(s):  
Chang-Hong Cheng ◽  
You-Lu Su ◽  
Hong-Ling Ma ◽  
Yi-Qin Deng ◽  
Juan Feng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Scylla Paramamosain ◽  
Mud Crab

Light intensity impacts on growth, molting and oxidative stress of juvenile mud crab Scylla paramamosain

Aquaculture ◽  
2021 ◽  
pp. 737159
Author(s):  
Shujian Chen ◽  
Herve Migaud ◽  
Ce Shi ◽  
Changbin Song ◽  
Chunlin Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Light Intensity ◽  
Scylla Paramamosain ◽  
Mud Crab ◽  
And Oxidative Stress
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