scholarly journals Characterization and Comparison of Intestinal Bacterial Microbiomes of Euschistus heros and Piezodorus guildinii Collected in Brazil and the United States

2021 ◽  
Vol 12 ◽  
Author(s):  
Matheus Sartori Moro ◽  
Xing Wu ◽  
Wei Wei ◽  
Lucas William Mendes ◽  
Kerry Clint Allen ◽  
...  
Keyword(s):  
United States ◽  
The United States ◽  
Microbial Pathogens ◽  
Intestinal Microbiome ◽  
Rrna Gene ◽  
Stink Bug ◽  
Stink Bugs ◽  
Physical Damage ◽  
Euschistus Heros ◽  
Piezodorus Guildinii

Background: Herbivorous insects are one of the main biological threats to crops. One such group of insects, stink bugs, do not eat large amounts of tissue when feeding on soybean, but are damaging to the quality of the seed yield as they feed on green developing seeds leading to poorly marketable harvests. In addition to causing physical damage during sucking-feeding activities, the insects can also transmit microbial pathogens, leading to even greater yield loss. Conducting surveys of the insect intestinal microbiome can help identify possible pathogens, as well as detail what healthy stink bug digestive systems have in common.Methods: We used the conserved V4 region of the 16S rRNA gene to characterize the bacterial microbiome of the red-banded stink bug Piezodorus guildinii collected in Brazil and the United States, as well as the neotropical brown stink bug Euschistus heros collected in Brazil.Results: After quality filtering of the data, 192 samples were kept for analyses: 117 samples from P. guildinii covering three sites in Brazil and four sites in the United States, and 75 samples for E. heros covering 10 sites in Brazil. The most interesting observations were that the diversity and abundance of some bacterial families were different in the different ecoregions of Brazil and the United States.Conclusion: Some families, such as Acetobacteraceae, Bacillaceae, Moraxellaceae, Enterobacteriaceae, and Rhodocyclaceae, may be related to the better adaptation in some localities in providing nutrients, break down cellulose, detoxify phytochemicals, and degrade organic compounds, which makes it difficult to control these species.

scholarly journals Characterization and Comparison of Intestinal Bacterial Microbiomes of Euschistus Heros and Piezodorus Guildinii Collected in Brazil and the United States

2021 ◽  
Author(s):  
Matheus Sartori Moro ◽  
Xing Wu ◽  
Wei Wei ◽  
Lucas William Mendes ◽  
Clint Allen ◽  
...  
Keyword(s):  
United States ◽  
The United States ◽  
Microbial Pathogens ◽  
Intestinal Microbiome ◽  
Rrna Gene ◽  
Stink Bug ◽  
Stink Bugs ◽  
Physical Damage ◽  
Euschistus Heros ◽  
Piezodorus Guildinii

Abstract Background: Herbaceous insects are one of the main biological threats to crops. One such group of insects, stink bugs, do not eat large amounts of tissue when feeding on soybean, but are extremely damaging to the quality of the seed yield as they feed directly on green developing seeds leading to poorly marketable harvests. In addition to causing physical damage to the seed during feeding, the insects can also transmit microbial pathogens, leading to even greater yield loss. Conducting surveys of the insect intestinal microbiome can help identify possible pathogens, as well as detail what healthy stink bug digestive systems have in common.Methods: We used the conserved V4 515-806 region of the 16S rRNA gene to characterize the bacterial microbiome of the red-banded stink bug Piezodorus guildinii collected in Brazil and the United States, as well as the neotropical brown stink bug Euschistus heros collected in Brazil.Results: After quality filtering of the data, 192 samples were kept for analyses: 117 samples from P. guildinii covering three sites in Brazil and four sites in the US, and 75 samples for E. heros covering 10 sites in Brazil. The most interesting observations were that the diversity and abundance of some bacterial families were different in the different ecoregions of Brazil and the United States.Conclusions: Some families may be related to the better adaptation in some localities in provide nutrients, break down cellulose, detoxify phytochemicals, and degrade organic compounds, which makes it difficult to control these species.

scholarly journals Characterization and Comparison of Intestinal Bacterial Microbiomes of Euschistus Heros and Piezodorus Guildinii Collected in Brazil and the United States

2021 ◽  
Author(s):  
Matheus Sartori Moro ◽  
Xing Wu ◽  
Wei Wei ◽  
Lucas William Mendes ◽  
Clint Allen ◽  
...  
Keyword(s):  
United States ◽  
The United States ◽  
Microbial Pathogens ◽  
Intestinal Microbiome ◽  
Rrna Gene ◽  
Stink Bug ◽  
Stink Bugs ◽  
Physical Damage ◽  
Euschistus Heros ◽  
Piezodorus Guildinii

Abstract Background: Herbaceous insects are one of the main biological threats to crops. One such group of insects, stink bugs, do not eat large amounts of tissue when feeding on soybean, but are extremely damaging to the quality of the seed yield as they feed directly on green developing seeds leading to poorly marketable harvests. In addition to causing physical damage to the seed during feeding, the insects can also transmit microbial pathogens, leading to even greater yield loss. Conducting surveys of the insect intestinal microbiome can help identify possible pathogens, as well as detail what healthy stink bug digestive systems have in common. Methods: We used the conserved V4 515-806 region of the 16S rRNA gene to characterize the bacterial microbiome of the red-banded stink bug Piezodorus guildinii collected in Brazil and the United States, as well as the neotropical brown stink bug Euschistus heros collected in Brazil. Results: After quality filtering of the data, 192 samples were kept for analyses: 117 samples from P. guildinii covering three sites in Brazil and four sites in the US, and 75 samples for E. heros covering 10 sites in Brazil. The most interesting observations were that the diversity and abundance of some bacterial families were different in the different ecoregions of Brazil and the United States. Conclusions: Some families may be related to the better adaptation in some localities in provide nutrients, break down cellulose, detoxify phytochemicals, and degrade organic compounds, which makes it difficult to control these species.

scholarly journals Pentatomids associated with blackberry

2014 ◽  
Vol 38 (3) ◽  
pp. 256-261
Author(s):  
Mauricio Paulo Batistella Pasini ◽  
Alessandro Dal'Col Lúcio
Keyword(s):  
Rio Grande ◽  
Nezara Viridula ◽  
Brown Spot ◽  
Stink Bug ◽  
Stink Bugs ◽  
Rio Grande Do Sul ◽  
Dark Brown Spot ◽  
Euschistus Heros ◽  
Santa Maria ◽  
Piezodorus Guildinii

Information concerning the presence of stink bugs in blackberry (Rubus spp.) in Brazil is sparse. This study aimed to identify the stink bug species associated with blackberry, to establish the daily dynamics and evaluate the fruits damage. The experiment was conducted in Santa Maria, Rio Grande do Sul State, Brazil, in blackberry orchard. Presence and evaluations of stink bugs were done weekly through visual and sweeping samplings at different day times. Five species of pentatomids were identified: Piezodorus guildinii, Nezara viridula, Euschistus heros, Dichelops furcatus and Edessa meditabunda. The bugs attack the drupelets producing a dark brown spot and wrinkled berries.

scholarly journals Community Composition, Abundance, and Phenology of Stink Bugs (Hemiptera: Pentatomidae) in Soybean in the North Central Region of the United States

2019 ◽  
Vol 112 (4) ◽  
pp. 1722-1731 ◽  
Author(s):  
Daniela T Pezzini ◽  
Christina D DiFonzo ◽  
Deborah L Finke ◽  
Thomas E Hunt ◽  
Janet J Knodel ◽  
...  
Keyword(s):  
United States ◽  
Central Region ◽  
The United States ◽  
Growth Stages ◽  
Stink Bug ◽  
North Central Region ◽  
Stink Bugs ◽  
North Central ◽  
The North

Abstract Stink bugs (Hemiptera: Pentatomidae) are an increasing threat to soybean (Fabales: Fabaceae) production in the North Central Region of the United States, which accounts for 80% of the country’s total soybean production. Characterization of the stink bug community is essential for development of management programs for these pests. However, the composition of the stink bug community in the region is not well defined. This study aimed to address this gap with a 2-yr, 9-state survey. Specifically, we characterized the relative abundance, richness, and diversity of taxa in this community, and assessed phenological differences in abundance of herbivorous and predatory stink bugs. Overall, the stink bug community was dominated by Euschistus spp. (Hemiptera: Pentatomidae) and Chinavia hilaris (Say) (Hemiptera: Pentatomidae). Euschistus variolarius (Palisot de Beauvois) (Hemiptera: Pentatomidae), C. hilaris and Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) were more abundant in the northwestern, southeastern and eastern parts, respectively, of the North Central Region of the United States. Economically significant infestations of herbivorous species occurred in fields in southern parts of the region. Species richness differed across states, while diversity was the same across the region. Herbivorous and predatory species were more abundant during later soybean growth stages. Our results represent the first regional characterization of the stink bug community in soybean fields and will be fundamental for the development of state- and region-specific management programs for these pests in the North Central Region of the United States.

scholarly journals Parasitism of Adult Pentatomidae by Tachinidae in Soybean in the North Central Region of the United States

2020 ◽  
Vol 20 (3) ◽  
Author(s):  
Pheylan A Anderson ◽  
Daniela T Pezzini ◽  
Nádia M Bueno ◽  
Christina D DiFonzo ◽  
Deborah L Finke ◽  
...  
Keyword(s):  
United States ◽  
Central Region ◽  
The United States ◽  
Stink Bug ◽  
North Central Region ◽  
Stink Bugs ◽  
North Central ◽  
The North ◽  
Percent Parasitism ◽  
Parasitism Rates

Abstract Stink bugs (Hemiptera: Pentatomidae) are agricultural pests of increasing significance in the North Central Region of the United States, posing a threat to major crops such as soybean. Biological control can reduce the need for insecticides to manage these pests, but the parasitism of stink bugs by Tachinidae (Diptera) is poorly characterized in this region. The objective of this study was to evaluate the rate of parasitism of stink bugs by tachinids over 2 yr from nine states across the North Central Region. Parasitism was assessed by quantifying tachinid eggs on the integument of stink bug adults. Parasitism rates (i.e., percent of adult stink bugs with tachinid eggs) were compared across stink bug species, states, stink bug sex, and years. The mean percent parasitism of stink bugs by tachinids was about 6% across the region and did not differ among stink bug species. Mean percent parasitism was significantly higher in Missouri than in northern and western states. In addition, male stink bugs had significantly higher mean percent parasitism than females. Stink bug species commonly found in soybean in the region showed some parasitism and are therefore potentially vulnerable to oviposition by these parasitoids. This is the first study to characterize the level of parasitism of stink bugs by tachinids across the North Central Region.

scholarly journals Diversity of stink bugs (Pentatomidae) in Chloris distichophylla Lag. (Poaceae) overwintering

2019 ◽  
Vol 1 ◽  
pp. ec01007
Author(s):  
Eduardo Engel ◽  
Mauricio P. B. Pasini
Keyword(s):  
Population Density ◽  
Rio Grande ◽  
Stink Bug ◽  
Stink Bugs ◽  
Rio Grande Do Sul ◽  
Euschistus Heros ◽  
Piezodorus Guildinii

The objective of this study was to evaluate the diversity and abundance of stink bugs in Chloris distichophylla Lag (Poales: Poaceae) during the soybean and corn off-season. The work was carried out in the municipality of Cruz Alta, Rio Grande do Sul state, over the soybean and corn off-season between 2014 and 2018. 3543 adults were identified into six species: Euschistus heros (Fabricius, 1798), Dichelops furcatus (Fabricius, 1775), Dichelops melacanthus (Dallas, 1851), Edessa meditabunda (Fabricus, 1794), Edessa ruformaginata (De Geer, 1773) and Piezodorus guildinii (Westwood, 1837) (Hemiptera: Pentatomidae). E. heros was the most abundant, followed by D. furcatus. The diameter of the clumps directly affects the population density of the stink bugs. Finally, C. distichophylla is shown as favorable to the maintenance of the stink bug populations over the soybean and corn off-season.

scholarly journals Impact of the Invasive Brown Marmorated Stink Bug,Halyomorpha halys(Stål), in Mid-Atlantic Tree Fruit Orchards in the United States: Case Studies of Commercial Management

2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Tracy C. Leskey ◽  
Brent D. Short ◽  
Bryan R. Butler ◽  
Starker E. Wright
Keyword(s):  
United States ◽  
The United States ◽  
Management Program ◽  
Brown Marmorated Stink Bug ◽  
Stink Bug ◽  
Stink Bugs ◽  
Tree Fruit ◽  
Economic Injury ◽  
Plautia Stali ◽  
Fruit Orchards

Four commercial orchards in the mid-Atlantic region of the United States were surveyed weekly in 2010 and 2011 for the presence of brown marmorated stink bug and the injury caused to both apple and peaches. Among tested sampling techniques, pyramid traps baited with the aggregation pheromone ofPlautia staliScott, methyl-(2E,4E,6Z)-decatrienoate, yielded the most brown marmorated stink bug adults and nymphs, followed by visual observations. Brown marmorated stink bugs began to feed on apples and peaches soon after fruit set and continued to feed on fruit throughout the growing season. Injury to apple was relatively inconsequential until after mid-June, whereas feeding on peaches resulted in immediate economic injury as the surface became distorted, dented, discolored, and the flesh beneath turned brown. Significantly more apples were injured and with greater severity in 2010 than in 2011. Likewise, percent injury on the exterior portion of each apple plot was significantly greater than injury reported from the interior in both years. Growers increased the number of insecticide applications nearly 4-fold from 2010 to 2011. In addition to the increased number of targeted insecticide applications, growers also reduced the interval between treatments in 2011. A metric was created to compare the relative intensity of each grower's commercial management program between seasons and amongst each other.

scholarly journals Spatial Patterns and Sequential Sampling Plans for Estimating Densities of Stink Bugs (Hemiptera: Pentatomidae) in Soybean in the North Central Region of the United States

2019 ◽  
Vol 112 (4) ◽  
pp. 1732-1740 ◽  
Author(s):  
Daniela T Pezzini ◽  
Christina D DiFonzo ◽  
Deborah L Finke ◽  
Thomas E Hunt ◽  
Janet J Knodel ◽  
...  
Keyword(s):  
United States ◽  
Central Region ◽  
Sequential Sampling ◽  
The United States ◽  
Stink Bug ◽  
North Central Region ◽  
Stink Bugs ◽  
Sampling Plans ◽  
North Central ◽  
The North

Abstract Stink bugs are an emerging threat to soybean (Fabales: Fabaceae) in the North Central Region of the United States. Consequently, region-specific scouting recommendations for stink bugs are needed. The aim of this study was to characterize the spatial pattern and to develop sampling plans to estimate stink bug population density in soybean fields. In 2016 and 2017, 125 fields distributed across nine states were sampled using sweep nets. Regression analyses were used to determine the effects of stink bug species [Chinavia hilaris (Say) (Hemiptera: Pentatomidae) and Euschistus spp. (Hemiptera: Pentatomidae)], life stages (nymphs and adults), and field locations (edge and interior) on spatial pattern as represented by variance–mean relationships. Results showed that stink bugs were aggregated. Sequential sampling plans were developed for each combination of species, life stage, and location and for all the data combined. Results for required sample size showed that an average of 40–42 sample units (sets of 25 sweeps) would be necessary to achieve a precision of 0.25 for stink bug densities commonly encountered across the region. However, based on the observed geographic gradient of stink bug densities, more practical sample sizes (5–10 sample units) may be sufficient in states in the southeastern part of the region, whereas impractical sample sizes (>100 sample units) may be required in the northwestern part of the region. Our findings provide research-based sampling recommendations for estimating densities of these emerging pests in soybean.

Stink Bugs in Florida Rice

EDIS ◽  
2013 ◽  
Vol 2013 (11) ◽  
Author(s):  
Ron Cherry
Keyword(s):  
United States ◽  
Dominant Species ◽  
The United States ◽  
Rice Production ◽  
Rice Fields ◽  
Stink Bug ◽  
Stink Bugs ◽  
First Report ◽  
Important Pest ◽  
Species Being

Although many different insects can be found in rice fields in Florida, stink bugs are currently considered the most important pest. Jones and Cherry reported that the rice stink bug was the dominant species, comprising more than 95% of the total stink bug population. Cherry et al. (1998) reported that the stink bug Oebalus ypsilongriseus was widespread in Florida rice fields. This was the first report of this species being found in commercial rice fields in the United States. Cherry and Nuessly (2010) reported that the stink bug Oebalus insularis is now widespread in Florida rice fields. This was the first report of this species being found in commercial rice fields in the United States. The stink bug complex attacking Florida rice is the most diversified and unique stink bug complex in US rice production. This 4-page fact sheet was written by Ron Cherry, and published by the UF Department of Entomology and Nematology, October 2013. http://edis.ifas.ufl.edu/in1009

scholarly journals Dermacentor variabilis is the Predominant Dermacentor spp. (Acari: Ixodidae) Feeding on Dogs and Cats Throughout the United States

2021 ◽  
Author(s):  
Kathryn T Duncan ◽  
Meriam N Saleh ◽  
Kellee D Sundstrom ◽  
Susan E Little
Keyword(s):  
United States ◽  
Spotted Fever ◽  
Rocky Mountain ◽  
The United States ◽  
Dermacentor Variabilis ◽  
Western United States ◽  
Its2 Region ◽  
Rrna Gene ◽  
Rocky Mountain Spotted Fever ◽  
Dermacentor Albipictus

Abstract Throughout North America, Dermacentor spp. ticks are often found feeding on animals and humans, and are known to transmit pathogens, including the Rocky Mountain spotted fever agent. To better define the identity and distribution of Dermacentor spp. removed from dogs and cats in the United States, ticks submitted from 1,457 dogs (n = 2,924 ticks) and 137 cats (n = 209 ticks) from veterinary practices in 44/50 states from February 2018-January 2020 were identified morphologically (n = 3,133); the identity of ticks from regions where Dermacentor andersoni (Stiles) have been reported, and a subset of ticks from other regions, were confirmed molecularly through amplification and sequencing of the ITS2 region and a 16S rRNA gene fragment. Of the ticks submitted, 99.3% (3,112/3,133) were Dermacentor variabilis (Say), 0.4% (12/3,133) were D. andersoni, and 0.3% (9/3,133) were Dermacentor albipictus (Packard). While translocation of pets prior to tick removal cannot be discounted, the majority (106/122; 87%) of Dermacentor spp. ticks removed from dogs and cats in six Rocky Mountain states (Montana, Idaho, Wyoming, Nevada, Utah, and Colorado) were D. variabilis, suggesting this species may be more widespread in the western United States than is currently recognized, or that D. andersoni, if still common in the region, preferentially feeds on hosts other than dogs and cats. Together, these data support the interpretation that D. variabilis is the predominant Dermacentor species found on pets throughout the United States, a finding that may reflect recent shifts in tick distribution.

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