Abstract
Background: Sarcopenia caused by spinal cord injury seriously affects the muscle function, which impairs the locomotion function of patients. As an effective physiotherapeutic, functional electrical stimulation is benefit to the recovery of muscle function without exact mechanisms. So the objection of the study is to explore the biological regulatory factors related to functional electrical stimulation for muscle function recovery of patients with sarcopenia after spinal cord injury using bioinformatics methods.Methods: The related microarray datasets GSE142426 and GSE33886 were downloaded from the Gene Expression Omnibus database. We used the R software to merge the two datasets, correct the inter-batch differences and screen the differentially expressed genes. Gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway were analyzed by using the DAVID online tool. The STRING database was used to analyze the interaction of differentially encoded proteins. The key genes were selected to draw the ROC curves, and CYCS was used to perform gene set enrichment analysis.Results: A total of 114 differentially expressed genes were selected, including 44 up-regulated genes and 70 down-regulated genes, and four hub genes were identified including CYCS, SUCLG1, ATP5B and ATP5C1. ROC curve showed that CYCS was considered as the best indicator and GSEA analysis showed that up-regulation of CYCS was related to mitochondria and energy metabolism.Conclusion: The mechanism of function electrical stimulation on muscle function recovery of spinal cord injury patients with sarcopenia is mainly related to regulate mitochondrial energy metabolism and scavenge reactive oxygen species to mitigate the oxidative damage.
Functional Electrical Stimulation: A Possible Strategy to Improve Muscle Function in Central Core Disease?