scholarly journals Identification of Olfactory Genes From the Greater Wax Moth by Antennal Transcriptome Analysis

2021 ◽  
Vol 12 ◽  
Author(s):  
Xing-Chuan Jiang ◽  
Su Liu ◽  
Xiu-Yun Jiang ◽  
Zheng-Wei Wang ◽  
Jin-Jing Xiao ◽  
...  
Keyword(s):  
Galleria Mellonella ◽  
Phylogenetic Analyses ◽  
Gene Clusters ◽  
Greater Wax Moth ◽  
Reverse Transcription Quantitative Pcr ◽  
Close Relationship ◽  
Transcriptome Resource ◽  
Lepidopteran Pest ◽  
Odorant Binding ◽  
Wax Moth

The olfactory system is used by insects to find hosts, mates, and oviposition sites. Insects have different types of olfactory proteins, including odorant-binding proteins (OBPs), chemosensory proteins (CSPs), odorant receptors (ORs), ionotropic receptors (IRs), and sensory neuron membrane proteins (SNMPs) to perceive chemical cues from the environment. The greater wax moth, Galleria mellonella, is an important lepidopteran pest of apiculture. However, the molecular mechanism underlying odorant perception in this species is unclear. In this study, we performed transcriptome sequencing of G. mellonella antennae to identify genes involved in olfaction. A total of 42,544 unigenes were obtained by assembling the transcriptome. Functional classification of these unigenes was determined by searching against the Gene Ontology (GO), eukaryotic orthologous groups (KOG), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. We identified a total of 102 olfactory-related genes: 21 OBPs, 18 CSPs, 43 ORs, 18 IRs, and 2 SNMPs. Results from BLASTX best hit and phylogenetic analyses showed that most of the genes had a close relationship with orthologs from other Lepidoptera species. A large number of OBPs and CSPs were tandemly arrayed in the genomic scaffolds and formed gene clusters. Reverse transcription-quantitative PCR results showed that GmelOBP19 and GmelOR47 are mainly expressed in male antennae. This work provides a transcriptome resource for olfactory genes in G. mellonella, and the findings pave the way for studying the function of these genes.

scholarly journals Establishing an invertebrate Galleria mellonella greater wax moth larval model of Neisseria gonorrhoeae infection

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1900-1920
Author(s):  
Aiste Dijokaite ◽  
Maria Victoria Humbert ◽  
Emma Borkowski ◽  
Roberto M La Ragione ◽  
Myron Christodoulides
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Wax Moth

Transgenic expression of gallerimycin, a novel antifungal insect defensin from the greater wax moth Galleria mellonella, confers resistance to pathogenic fungi in tobacco

2006 ◽  
Vol 387 (5) ◽  
pp. 549-557 ◽  
Author(s):  
Gregor Langen ◽  
Jafargholi Imani ◽  
Boran Altincicek ◽  
Gernot Kieseritzky ◽  
Karl-Heinz Kogel ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Fungal Pathogens ◽  
Galleria Mellonella ◽  
Ectopic Expression ◽  
Pathogenic Fungi ◽  
Transgenic Expression ◽  
Greater Wax Moth ◽  
Insect Defensin ◽  
Wax Moth

Abstract A cDNA encoding gallerimycin, a novel antifungal peptide from the greater wax moth Galleria mellonella, was isolated from a cDNA library of genes expressed during innate immune response in the caterpillars. Upon ectopic expression of gallerimycin in tobacco, using Agrobacterium tumefaciens as a vector, gallerimycin conferred resistance to the fungal pathogens Erysiphe cichoracearum and Sclerotinia minor. Quantification of gallerimycin mRNA in transgenic tobacco by real-time PCR confirmed transgenic expression under control of the inducible mannopine synthase promoter. Leaf sap and intercellular washing fluid from transgenic tobacco inhibited in vitro germination and growth of the fungal pathogens, demonstrating that gallerimycin is secreted into intercellular spaces. The feasibility of the use of gallerimycin to counteract fungal diseases in crop plants is discussed.

Greater wax moth Galleria mellonella (Lepidoptera: Pyralidae) as a resistant model host for Nosema pyrausta (Microsporidia: Nosematidae)

2018 ◽  
Vol 157 ◽  
pp. 1-3 ◽  
Author(s):  
Yuri S. Tokarev ◽  
Ekaterina V. Grizanova ◽  
Anastasia N. Ignatieva ◽  
Ivan M. Dubovskiy
Keyword(s):  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Lepidoptera Pyralidae ◽  
Wax Moth

scholarly journals Quantitative and Qualitative Impacts of Selected Arthropod Venoms on the Larval Haemogram of the Greater Wax Moth, Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae)

2021 ◽  
pp. 1-26
Author(s):  
Karem Ghoneim ◽  
Khalid Hamadah ◽  
Mohammad Tanani ◽  
Dyaa Emam
Keyword(s):  
Apis Mellifera ◽  
Galleria Mellonella ◽  
Larval Instar ◽  
Population Count ◽  
Oriental Hornet ◽  
Greater Wax Moth ◽  
The World ◽  
Lepidoptera Pyralidae ◽  
Hemocyte Population ◽  
Wax Moth

The greater wax moth, Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) is the most destructive pest of honey bee, Apis mellifera Linnaeus (Hymenoptera: Apidae), throughout the world. The present study was conducted to determine the quantitative and qualitative impairing effects of the arthropod venoms, viz., death stalker scorpion Leiurus quinquestriatus (Hemprich & Ehrenberg) venom (SV), oriental Hornet (wasp) Vespa orientalis Linnaeus venom (WV) and Apitoxin of A. mellifera (AP) on the larval haemogram. For this purpose, the 3rd instar larvae were treated with LC50 of each of these venoms (3428.9, 2412.6, and 956.16 ppm, respectively). The haematological investigation was conducted in haemolymph of the 5th and 7th (last) instar larvae. The important results could be summarized as follows. Five basic types of the freely circulating haemocytes in the haemolymph of last instar (7th) larvae of G. mellonella had been identified: Prohemocytes (PRs), Plasmatocytes (PLs), Granulocytes (GRs), Spherulocytes (SPs) and Oenocytoids (OEs). All venoms unexceptionally prohibited the larvae to produce normal hemocyte population (count). No certain trend of disturbance in the differential hemocyte counts of circulating hemocytes in larvae of G. mellonella after treatment with the arthropod venoms. Increasing or decreasing population of the circulating hemocytes seemed to depend on the potency of the venom, hemocyte type and the larval instar.  In PRs of last instar larvae, some cytopathological features had been observed after treatment with AP or WV, but SV failed to cause cytopathological features. With regard to PLs, some cytopathological features had been observed after treatment with AP while both SV and WV failed to cause cytopathological features in this hemocyte type. No venom exhibited cytopathological effects on GRs, SPs or OEs.

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