Evaluation of Colostral Immunity Against Equine Herpesvirus Type 1 (EHV-1) in Martina Franca's Foals

Eight Martina Franca pregnant jennies were selected in order to evaluate the transfer of colostral antibodies against equine herpesvirus type 1 in their relative foals after immunization with a commercial inactivated vaccine, compared with an unvaccinated group. Samples of serum and colostrums/milk were collected from jennies and foals under study starting from 10 min before and up to 21 days after the foaling. Specific anti-EHV-1 antibody titers were evaluated by means of a serum neutralization test, and the results obtained from both groups were analyzed. The serological titers in the vaccinated jennies was significantly higher (p < 0.01). No significant differences were found in the specific time-point intervals in both groups examined (p > 0.05). The antibody titers in milk at the time of delivery and subsequent withdrawal (T0 and T1) were very high in both groups, but no significant differences were found between the two groups (p > 0.05). In the foal sera, a significant difference was found between foals in the vaccinated group compared with those in the unvaccinated group (p < 0.05). Finally, a significant correlation (p < 0.05) was observed between the antibody titers found in serum and colostrum of jennies and the foal titers in the first time-point sampling (up to 12 h after foaling). The results confirm a substantial homology in the antibody production compared with other most investigated equids, highlighting the efficacy of the vaccination against EHV-1 of the jennies to ensure the protective immunity to their foals during the first weeks after delivery.

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Biochemical transformation of deoxythymidine kinase-deficient mouse cells with UV-irradiated equine herpesvirus type 1.

Journal of Virology ◽

10.1128/jvi.28.1.361-367.1978 ◽

1978 ◽

Vol 28 (1) ◽

pp. 361-367 ◽

Cited By ~ 8

Author(s):

G P Allen ◽

J J McGowan ◽

G A Gentry ◽

C C Randall

Keyword(s):

Equine Herpesvirus ◽

Deficient Mouse ◽

Herpesvirus Type ◽

Equine Herpesvirus Type ◽

Equine Herpesvirus Type 1 ◽

Mouse Cells

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Decreased virus-neutralizing antibodies against equine herpesvirus type 1 in nasal secretions of horses after 12-hour transportation

Journal of Equine Veterinary Science ◽

10.1016/j.jevs.2021.103665 ◽

2021 ◽

pp. 103665

Author(s):

Hiroshi Bannai ◽

Yuji Takahashi ◽

Hajime Ohmura ◽

Yusaku Ebisuda ◽

Kazutaka Mukai ◽

...

Keyword(s):

Neutralizing Antibodies ◽

Equine Herpesvirus ◽

Herpesvirus Type ◽

Equine Herpesvirus Type ◽

Equine Herpesvirus Type 1 ◽

Nasal Secretions

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Evaluation of a vectored equine herpesvirus type 1 (EHV-1) vaccine expressing H3 haemagglutinin in the protection of dogs against canine influenza

Vaccine ◽

10.1016/j.vaccine.2008.02.064 ◽

2008 ◽

Vol 26 (19) ◽

pp. 2335-2343 ◽

Cited By ~ 24

Author(s):

Cristina Rosas ◽

Gerlinde R. Van de Walle ◽

Stephan M. Metzger ◽

Karin Hoelzer ◽

Edward J. Dubovi ◽

...

Keyword(s):

Equine Herpesvirus ◽

Herpesvirus Type ◽

Equine Herpesvirus Type ◽

Equine Herpesvirus Type 1 ◽

Canine Influenza

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A touchdown PCR for the differentiation of equine herpesvirus type 1 (EHV-1) field strains from the modified live vaccine strain RacH

Journal of Virological Methods ◽

10.1016/0166-0934(94)90169-4 ◽

1994 ◽

Vol 50 (1-3) ◽

pp. 129-136 ◽

Cited By ~ 14

Author(s):

Nikolaus Osterrieder ◽

Peter H. Hübert ◽

Christine Brandmüller ◽

Oskar-Rüger Kaaden

Keyword(s):

Vaccine Strain ◽

Live Vaccine ◽

Live Vaccine Strain ◽

Equine Herpesvirus ◽

Herpesvirus Type ◽

Equine Herpesvirus Type ◽

Equine Herpesvirus Type 1 ◽

Touchdown Pcr ◽

Modified Live Vaccine

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Late-Term Abortion, Stillbirth, and Neonatal Foal Death in Kyrgyzstan: First Isolation of Equine Herpesvirus Type 1 in the Country

Journal of Equine Veterinary Science ◽

10.1016/j.jevs.2016.12.008 ◽

2017 ◽

Vol 51 ◽

pp. 46-53

Author(s):

Maksat Akhmedzhanov ◽

Rysbek Nurgaziev ◽

Jailobek Orozov ◽

Irmgard Moser ◽

Nikolaus Osterrieder ◽

...

Keyword(s):

Equine Herpesvirus ◽

Herpesvirus Type ◽

Equine Herpesvirus Type ◽

Equine Herpesvirus Type 1

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Recombinant Glycoprotein B of Equine herpesvirus Type 1 Elicits Protective Immune Response against Challenge in BALB/c Mouse Model

Indian Journal of Animal Research ◽

10.18805/ijar.b-4373 ◽

2021 ◽

Author(s):

Alok Joshi ◽

R.P. Gupta ◽

Selvaraj Pavulraj ◽

Bidhan Chandra Bera ◽

Taruna Anand ◽

...

Keyword(s):

Immune Response ◽

Plasmid Dna ◽

Protective Immune Response ◽

Glycoprotein B ◽

Equine Herpesvirus ◽

Recombinant Glycoprotein ◽

Herpesvirus Type ◽

Equine Herpesvirus Type ◽

Equine Herpesvirus Type 1

Background: Equine herpesvirus type 1 (EHV-1) is the most important viral pathogen of equines, causing respiratory illness, abortion, neonatal foal mortality and neurologic disorders. Large numbers of commercial EHV-1 vaccines are available to protect equines from the disease, but they provide only partial protection. Despite immunization with inactivated and modified live virus vaccine, mares show abortions. Present study was aimed to investigate the immunogenicity and protective efficacy of EHV-1 recombinant glycoprotein B (rgB) and gB expressing plasmid DNA against EHV-1 infection in BALB/c mice model.Methods: About 3-4 weeks old 225 female BALB/c mice were selected for the comparative study of immunization followed by challenged with EHV-1/India/Tohana/96-2 strain virus in 5 different groups of 45 animals each.Result: Following immunization, rgB vaccinated mice showed optimal stimulation of EHV-1 gB specific cell mediated and humoral mediated immunity (HMI and CMI). The gB expressing plasmid DNA vaccinated mice developed only CMI while inactivated whole virus vaccinated mice had only HMI. Upon EHV-1 challenge, all infected mice displayed variable levels of clinical signs with changes in body weight, however, vaccinated mice showed very rapid recovery with optimal protection. Positive control group mice showed severe pulmonary lesions along with persistence virus infection till 5 days post challenge (dpc) whereas vaccinated mice had less pulmonary lesion only up to 3dpc. Minimal lung lesions and early virus clearance was observed in the rgB immunized mice in comparison to the gB plasmid DNA and inactivated EHV-1 vaccine immunized mice. It has been concluded that immunization with rgB elicits optimum protective immune response against EHV-1 infection in mice model. The rgB could be a potential vaccine candidate against EHV-1 infection in equine in the future.

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