scholarly journals T329S Mutation in the FMO3 Gene Alleviates Lipid Metabolic Diseases in Chickens in the Late Laying Period

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 48
Author(s):  
Jianlou Song ◽  
Mingyi Huang ◽  
Xuefeng Shi ◽  
Xianyu Li ◽  
Xia Chen ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Serum Lipid ◽  
Metabolic Diseases ◽  
Laying Hens ◽  
Total Bile Acid ◽  
Production Performance ◽  
Atherosclerotic Lesions ◽  
Similar Body ◽  
Lipid Transporter ◽  
Lipid Parameters

The T329S mutation in flavin-containing monooxygenase 3 (FMO3) impairs the trimethylamine (TMA) metabolism in laying hens. The TMA metabolic pathway is closely linked to lipid metabolic diseases, such as atherosclerosis and fatty liver disease. We aimed to evaluate the effects of the T329S mutation in FMO3 on lipid metabolism in chickens during the late laying period. We selected 18 FMO3 genotyped individuals (consisting of six AA, six AT, and six TT hens) with similar body weight and production performance. The lipid metabolism and deposition characteristics of the laying hens with different genotypes were compared. The T329S mutation moderated the serum-lipid parameters in TT hens compared to those in AA and AT hens from 49 to 62 weeks. Furthermore, it reduced the serum trimethylamine N-oxide concentrations and increased the serum total bile acid (p < 0.05) and related lipid transporter levels in TT hens. Moreover, it significantly (p < 0.01) decreased atherosclerotic lesions and hepatic steatosis in TT hens compared to those in the AA and AT hens. Our findings may help improve the health status in laying hens during the late laying period.

scholarly journals Effect of Chain Length and Saturation of the Fatty Acids in Dietary Triglycerides on Lipid Metabolism in Wistar Rats

2020 ◽  
Author(s):  
Chaturi M. Senanayake ◽  
Harsha Hapugaswatta ◽  
Gangi Samarawickrama ◽  
Nimanthi Jayathilaka ◽  
Kapila Seneviratne
Keyword(s):  
Fatty Acids ◽  
Regression Analysis ◽  
Lipid Metabolism ◽  
Chain Length ◽  
Serum Lipid ◽  
Wistar Rats ◽  
Degree Of Saturation ◽  
Hepatic Lipid Metabolism ◽  
Hepatic Lipid ◽  
Lipid Parameters

<div>Dietary fatty acids are associated with lipid health. We investigated the effect of the chain length </div><div>and the degree of saturation of fatty acids in dietary triglycerides on serum lipid profiles and </div><div>hepatic lipid metabolism in Wistar rats. Fat component of the basal diet (soybean oil) was </div><div>replaced with fats with fatty acids of different chain lengths and saturation and the serum lipids </div><div>were monitored for 150 days. Principal component (PC) analysis of serum lipid components </div><div>were related to chain length and saturation using second order polynomial regression analysis. </div><div>The combined effect of chain length and saturation on PC 1 scores were evaluated by multiple </div><div>regression analysis. Variation of lipid parameters cannot be well-explained by chain length or </div><div>saturation alone. Consistent with the formation of large amounts of lipid droplets in the liver, </div><div>expression of sterol regulatory element binding protein -2 (SREBP2) and peroxisome </div><div>proliferator-activated receptors (PPARα) involved in hepatic lipid metabolism showed </div><div>significant (P<0.05) downregulation in margarine diet group and SREBP2 in dairy butter diet </div><div>group compared to the control group. Average chain length of fatty acids in triglycerides has a </div><div>higher influence on the quality of serum lipid parameters than the average degree of saturation of </div><div>fatty acids.</div>

scholarly journals Transcriptome Analysis Reveals Mechanism Underlying the Differential Intestinal Functionality of Laying Hens in the Late Phase and Peak Phase of Production

2019 ◽  
Author(s):  
Weiwei Wang ◽  
Jing Wang ◽  
Haijun Zhang ◽  
Shugeng Wu ◽  
Guanghai Qi
Keyword(s):  
Lipid Metabolism ◽  
Laying Hens ◽  
Late Phase ◽  
Antioxidant Property ◽  
Production Performance ◽  
Glutathione Metabolism ◽  
Distal Small Intestine ◽  
Ppar Signaling ◽  
Upregulated Genes ◽  
Peak Phase

Abstract Background: The compromised performance of laying hens in the late phase of production relative to the peak production was thought to be associated with the impairment of intestinal functionality, which plays essential roles in contributing to their overall health and production performance. In the present study, RNA sequencing was used to investigate differences in the expression profile of intestinal functionality-related genes and associated pathways between laying hens in the late phase and peak phase of production. Results : A total of 104 upregulated genes with 190 downregulated genes were identified in the ileum (the distal small intestine) of laying hens in the late phase of production compared to those at peak production. These upregulated genes were found to be enriched in little KEGG pathway, however, the downregulated genes were enriched in the pathways of PPAR signaling pathway, oxidative phosphorylation and glutathione metabolism. Besides, these downregulated genes were mapped to several GO clusters in relation to lipid metabolism, electron transport of respiratory chain, and oxidation resistance. Similarly, there were lower activities of total superoxide dismutase, glutathione S-transferase and Na + /K + -ATPase, and reductions of total antioxidant capacity and ATP level, along with an elevation in malondialdehyde content in the ileum of laying hens in the late phase of production as compared with those at peak production. Conclusions: The intestine of laying hens in the late phase of production were predominantly characterized by a disorder of lipid metabolism, concurrent with impairments of energy production and antioxidant property. This study uncovers the mechanism underlying differences between the intestinal functionality of laying hens in the late phase and peak phase of production, thereby providing potential targets for the genetic control or dietary modulation of intestinal hypofunction of laying hens in the late phase of production.

scholarly journals Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders

2021 ◽  
Vol 8 ◽  
Author(s):  
Qiuhua Yang ◽  
Qian Ma ◽  
Jiean Xu ◽  
Zhiping Liu ◽  
Jianqiu Zou ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Metabolic Disorders ◽  
Metabolic Diseases ◽  
Myeloid Cells ◽  
Glucose And Lipid Metabolism ◽  
Atherosclerotic Lesions ◽  
Its Gene ◽  
Metabolic Sensor ◽  
Amp Activated Protein Kinase ◽  
Macrophage Viability

Myeloid cells, including monocytes/macrophages, primarily rely on glucose and lipid metabolism to provide the energy and metabolites needed for their functions and survival. AMP-activated protein kinase (AMPK, its gene is PRKA for human, Prka for rodent) is a key metabolic sensor that regulates many metabolic pathways. We studied recruitment and viability of Prkaa1-deficient myeloid cells in mice and the phenotype of these mice in the context of cardio-metabolic diseases. We found that the deficiency of Prkaa1 in myeloid cells downregulated genes for glucose and lipid metabolism, compromised glucose and lipid metabolism of macrophages, and suppressed their recruitment to adipose, liver and arterial vessel walls. The viability of macrophages in the above tissues/organs was also decreased. These cellular alterations resulted in decreases in body weight, insulin resistance, and lipid accumulation in liver of mice fed with a high fat diet, and reduced the size of atherosclerotic lesions of mice fed with a Western diet. Our results indicate that AMPKα1/PRKAA1-regulated metabolism supports monocyte recruitment and macrophage viability, contributing to the development of diet-induced metabolic disorders including diabetes and atherosclerosis.

scholarly journals Transcriptome Analysis Reveals Mechanism Underlying the Differential Intestinal Functionality of Laying Hens in the Late Phase and Peak Phase of Production

2019 ◽  
Author(s):  
Weiwei Wang ◽  
Jing Wang ◽  
Haijun Zhang ◽  
Shugeng Wu ◽  
Guanghai Qi
Keyword(s):  
Lipid Metabolism ◽  
Laying Hens ◽  
Late Phase ◽  
Antioxidant Property ◽  
Production Performance ◽  
Glutathione Metabolism ◽  
Distal Small Intestine ◽  
Ppar Signaling ◽  
Upregulated Genes ◽  
Peak Phase

Abstract Background: The compromised performance of laying hens in the late phase of production relative to the peak production was thought to be associated with the impairment of intestinal functionality, which plays essential roles in contributing to their overall health and production performance. In the present study, RNA sequencing was used to investigate differences in the expression profile of intestinal functionality-related genes and associated pathways between laying hens in the late phase and peak phase of production. Results : A total of 104 upregulated genes with 190 downregulated genes were identified in the ileum (the distal small intestine) of laying hens in the late phase of production compared to those at peak production. These upregulated genes were found to be enriched in little KEGG pathway, however, the downregulated genes were enriched in the pathways of PPAR signaling pathway, oxidative phosphorylation and glutathione metabolism. Besides, these downregulated genes were mapped to several GO clusters in relation to lipid metabolism, electron transport of respiratory chain, and oxidation resistance. Similarly, there were lower activities of total superoxide dismutase, glutathione S-transferase and Na + /K + -ATPase, and reductions of total antioxidant capacity and ATP level, along with an elevation in malondialdehyde content in the ileum of laying hens in the late phase of production as compared with those at peak production. Conclusions: The intestine of laying hens in the late phase of production were predominantly characterized by a disorder of lipid metabolism, concurrent with impairments of energy production and antioxidant property. This study uncovers the mechanism underlying differences between the intestinal functionality of laying hens in the late phase and peak phase of production, thereby providing potential targets for the genetic control or dietary modulation of intestinal hypofunction of laying hens in the late phase of production.

Effect of Chain Length and Saturation of the Fatty Acids in Dietary Triglycerides on Lipid Metabolism in Wistar Rats

2020 ◽  
Author(s):  
Chaturi M. Senanayake ◽  
Harsha Hapugaswatta ◽  
Gangi Samarawickrama ◽  
Nimanthi Jayathilaka ◽  
Kapila Seneviratne
Keyword(s):  
Fatty Acids ◽  
Regression Analysis ◽  
Lipid Metabolism ◽  
Chain Length ◽  
Serum Lipid ◽  
Wistar Rats ◽  
Degree Of Saturation ◽  
Hepatic Lipid Metabolism ◽  
Hepatic Lipid ◽  
Lipid Parameters

<div>Dietary fatty acids are associated with lipid health. We investigated the effect of the chain length </div><div>and the degree of saturation of fatty acids in dietary triglycerides on serum lipid profiles and </div><div>hepatic lipid metabolism in Wistar rats. Fat component of the basal diet (soybean oil) was </div><div>replaced with fats with fatty acids of different chain lengths and saturation and the serum lipids </div><div>were monitored for 150 days. Principal component (PC) analysis of serum lipid components </div><div>were related to chain length and saturation using second order polynomial regression analysis. </div><div>The combined effect of chain length and saturation on PC 1 scores were evaluated by multiple </div><div>regression analysis. Variation of lipid parameters cannot be well-explained by chain length or </div><div>saturation alone. Consistent with the formation of large amounts of lipid droplets in the liver, </div><div>expression of sterol regulatory element binding protein -2 (SREBP2) and peroxisome </div><div>proliferator-activated receptors (PPARα) involved in hepatic lipid metabolism showed </div><div>significant (P<0.05) downregulation in margarine diet group and SREBP2 in dairy butter diet </div><div>group compared to the control group. Average chain length of fatty acids in triglycerides has a </div><div>higher influence on the quality of serum lipid parameters than the average degree of saturation of </div><div>fatty acids.</div>

scholarly journals Transcriptome Analysis Reveals Mechanism Underlying the Differential Intestinal Functionality of Laying Hens in the Late Phase and Peak Phase of Production

2019 ◽  
Author(s):  
Weiwei Wang ◽  
Jing Wang ◽  
Haijun Zhang ◽  
Shugeng Wu ◽  
Guanghai Qi
Keyword(s):  
Lipid Metabolism ◽  
Laying Hens ◽  
Late Phase ◽  
Antioxidant Property ◽  
Production Performance ◽  
Glutathione Metabolism ◽  
Ppar Signaling ◽  
Total Antioxidant ◽  
Upregulated Genes ◽  
Peak Phase

Abstract Background The compromised performance of laying hens in the late phase of production relative to the peak production was thought to be associated with the impairment of intestinal functionality, which plays essential roles in contributing to their overall health and production performance. In the present study, RNA sequencing was used to investigate differences in the expression profile of intestinal functionality-related genes and associated pathways between layers in the late phase and peak phase of production. Results A total of 104 upregulated genes with 190 downregulated genes were identified in the ileum of layers in the late phase of production compared to those at peak production. These upregulated genes were found to be enriched in little KEGG pathway, however, the downregulated genes were enriched in the pathways of PPAR signaling pathway, oxidative phosphorylation and glutathione metabolism. Besides, these downregulated genes were mapped to several GO clusters in relation to lipid metabolism, electron transport of respiratory chain, and oxidation resistance. Similarly, there were lower activities of total superoxide dismutase, glutathione S-transferase and Na+/K+-ATPase, and reductions of total antioxidant capacity and ATP level, along with an elevation in malondialdehyde content in the ileum of layers in the late phase of production as compared with those at peak production. Conclusions The intestine of layers in the late phase of production were predominantly characterized by a disorder of lipid metabolism, concurrent with impairments of energy production and antioxidant property. This study uncovers the mechanism underlying differences between the intestinal functionality of layers in the late phase and peak phase of production, thereby providing potential targets for the genetic control or dietary modulation of intestinal hypofunction of layers in the late phase of production.

scholarly journals Transcriptome analysis reveals mechanism underlying the differential intestinal functionality of laying hens in the late phase and peak phase of production

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Wei-wei Wang ◽  
Jing Wang ◽  
Hai-jun Zhang ◽  
Shu-geng Wu ◽  
Guang-hai Qi
Keyword(s):  
Lipid Metabolism ◽  
Laying Hens ◽  
Late Phase ◽  
Antioxidant Property ◽  
Production Performance ◽  
Glutathione Metabolism ◽  
Distal Small Intestine ◽  
Ppar Signaling ◽  
Upregulated Genes ◽  
Peak Phase

Abstract Background The compromised performance of laying hens in the late phase of production relative to the peak production was thought to be associated with the impairment of intestinal functionality, which plays essential roles in contributing to their overall health and production performance. In the present study, RNA sequencing was used to investigate differences in the expression profile of intestinal functionality-related genes and associated pathways between laying hens in the late phase and peak phase of production. Results A total of 104 upregulated genes with 190 downregulated genes were identified in the ileum (the distal small intestine) of laying hens in the late phase of production compared to those at peak production. These upregulated genes were found to be enriched in little KEGG pathway, however, the downregulated genes were enriched in the pathways of PPAR signaling pathway, oxidative phosphorylation and glutathione metabolism. Besides, these downregulated genes were mapped to several GO clusters in relation to lipid metabolism, electron transport of respiratory chain, and oxidation resistance. Similarly, there were lower activities of total superoxide dismutase, glutathione S-transferase and Na+/K+-ATPase, and reductions of total antioxidant capacity and ATP level, along with an elevation in malondialdehyde content in the ileum of laying hens in the late phase of production as compared with those at peak production. Conclusions The intestine of laying hens in the late phase of production were predominantly characterized by a disorder of lipid metabolism, concurrent with impairments of energy production and antioxidant property. This study uncovers the mechanism underlying differences between the intestinal functionality of laying hens in the late phase and peak phase of production, thereby providing potential targets for the genetic control or dietary modulation of intestinal hypofunction of laying hens in the late phase of production.

scholarly journals Transcriptome Analysis Reveals Mechanism Underlying the Differential Intestinal Functionality of Laying Hens in the Late Phase and Peak Phase of Production

2019 ◽  
Author(s):  
Weiwei Wang ◽  
Jing Wang ◽  
Haijun Zhang ◽  
Shugeng Wu ◽  
Guanghai Qi
Keyword(s):  
Lipid Metabolism ◽  
Laying Hens ◽  
Late Phase ◽  
Antioxidant Property ◽  
Production Performance ◽  
Glutathione Metabolism ◽  
Ppar Signaling ◽  
Total Antioxidant ◽  
Upregulated Genes ◽  
Peak Phase

Abstract Background The compromised performance of laying hens in the late phase of production relative to the peak production was thought to be associated with the impairment of intestinal functionality, which plays essential roles in contributing to their overall health and production performance. In the present study, RNA sequencing was used to investigate differences in the expression profile of intestinal functionality-related genes and associated pathways between layers in the late phase and peak phase of production. Results A total of 104 upregulated genes with 190 downregulated genes were identified in the ileum of layers in the late phase of production compared to those at peak production. These upregulated genes were found to be enriched in little KEGG pathway, however, the downregulated genes were enriched in the pathways of PPAR signaling pathway, oxidative phosphorylation and glutathione metabolism. Besides, these downregulated genes were mapped to several GO clusters in relation to lipid metabolism, electron transport of respiratory chain, and oxidation resistance. Similarly, there were lower activities of total superoxide dismutase, glutathione S-transferase and Na+/K+-ATPase, and reductions of total antioxidant capacity and ATP level, along with an elevation in malondialdehyde content in the ileum of layers in the late phase of production as compared with those at peak production. Conclusions The intestine of layers in the late phase of production were predominantly characterized by a disorder of lipid metabolism, concurrent with impairments of energy production and antioxidant property. This study uncovers the mechanism underlying differences between the intestinal functionality of layers in the late phase and peak phase of production, thereby providing potential targets for the genetic control or dietary modulation of intestinal hypofunction of layers in the late phase of production.

scholarly journals Transcriptome Analysis Reveals Mechanism Underlying the Differential Intestinal Functionality of Laying Hens in the Late Phase and Peak Phase of Production

2019 ◽  
Author(s):  
Weiwei Wang ◽  
Jing Wang ◽  
Haijun Zhang ◽  
Shugeng Wu ◽  
Guanghai Qi
Keyword(s):  
Lipid Metabolism ◽  
Laying Hens ◽  
Late Phase ◽  
Antioxidant Property ◽  
Production Performance ◽  
Glutathione Metabolism ◽  
Distal Small Intestine ◽  
Ppar Signaling ◽  
Upregulated Genes ◽  
Peak Phase

Abstract Background: The compromised performance of laying hens in the late phase of production relative to the peak production was thought to be associated with the impairment of intestinal functionality, which plays essential roles in contributing to their overall health and production performance. In the present study, RNA sequencing was used to investigate differences in the expression profile of intestinal functionality-related genes and associated pathways between laying hens in the late phase and peak phase of production. Results : A total of 104 upregulated genes with 190 downregulated genes were identified in the ileum (the distal small intestine) of laying hens in the late phase of production compared to those at peak production. These upregulated genes were found to be enriched in little KEGG pathway, however, the downregulated genes were enriched in the pathways of PPAR signaling pathway, oxidative phosphorylation and glutathione metabolism. Besides, these downregulated genes were mapped to several GO clusters in relation to lipid metabolism, electron transport of respiratory chain, and oxidation resistance. Similarly, there were lower activities of total superoxide dismutase, glutathione S-transferase and Na + /K + -ATPase, and reductions of total antioxidant capacity and ATP level, along with an elevation in malondialdehyde content in the ileum of laying hens in the late phase of production as compared with those at peak production. C onclusion s: The intestine of laying hens in the late phase of production were predominantly characterized by a disorder of lipid metabolism, concurrent with impairments of energy production and antioxidant property. This study uncovers the mechanism underlying differences between the intestinal functionality of laying hens in the late phase and peak phase of production, thereby providing potential targets for the genetic control or dietary modulation of intestinal hypofunction of laying hens in the late phase of production.

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