scholarly journals Tailored Functionalized Magnetic Nanoparticles to Target Breast Cancer Cells Including Cancer Stem-Like Cells

Cancers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1397
Author(s):  
Ana Lazaro-Carrillo ◽  
Macarena Calero ◽  
Antonio Aires ◽  
Aitziber L. Cortajarena ◽  
Bruno M. Simões ◽  
...  

Nanotechnology-based approaches hold substantial potential to avoid chemoresistance and minimize side effects. In this work, we have used biocompatible iron oxide magnetic nanoparticles (MNPs) called MF66 and functionalized with the antineoplastic drug doxorubicin (DOX) against MDA-MB-231 cells. Electrostatically functionalized MNPs showed effective uptake and DOX linked to MNPs was more efficiently retained inside the cells than free DOX, leading to cell inactivation by mitotic catastrophe, senescence and apoptosis. Both effects, uptake and cytotoxicity, were demonstrated by different assays and videomicroscopy techniques. Likewise, covalently functionalized MNPs using three different linkers—disulfide (DOX-S-S-Pyr, called MF66-S-S-DOX), imine (DOX-I-Mal, called MF66-I-DOX) or both (DOX-I-S-S-Pyr, called MF66-S-S-I-DOX)—were also analysed. The highest cell death was detected using a linker sensitive to both pH and reducing environment (DOX-I-S-S-Pyr). The greatest success of this study was to detect also their activity against breast cancer stem-like cells (CSC) from MDA-MB-231 and primary breast cancer cells derived from a patient with a similar genetic profile (triple-negative breast cancer). In summary, these nanoformulations are promising tools as therapeutic agent vehicles, due to their ability to produce efficient internalization, drug delivery, and cancer cell inactivation, even in cancer stem-like cells (CSCs) from patients.

2017 ◽  
Vol 44 (6) ◽  
pp. 886-894 ◽  
Author(s):  
Hamed Nosrati ◽  
Marziyeh Salehiabar ◽  
Soodabeh Davaran ◽  
Hossein Danafar ◽  
Hamidreza Kheiri Manjili

2006 ◽  
Vol 26 (8) ◽  
pp. 1451-1455 ◽  
Author(s):  
J. Zhou ◽  
C. Leuschner ◽  
C. Kumar ◽  
J. Hormes ◽  
W.O. Soboyejo

2021 ◽  
Vol 14 (2) ◽  
pp. 169
Author(s):  
Gloria Ana ◽  
Patrick M. Kelly ◽  
Azizah M. Malebari ◽  
Sara Noorani ◽  
Seema M. Nathwani ◽  
...  

We report the synthesis and biochemical evaluation of compounds that are designed as hybrids of the microtubule targeting benzophenone phenstatin and the aromatase inhibitor letrozole. A preliminary screening in estrogen receptor (ER)-positive MCF-7 breast cancer cells identified 5-((2H-1,2,3-triazol-1-yl)(3,4,5-trimethoxyphenyl)methyl)-2-methoxyphenol 24 as a potent antiproliferative compound with an IC50 value of 52 nM in MCF-7 breast cancer cells (ER+/PR+) and 74 nM in triple-negative MDA-MB-231 breast cancer cells. The compounds demonstrated significant G2/M phase cell cycle arrest and induction of apoptosis in the MCF-7 cell line, inhibited tubulin polymerisation, and were selective for cancer cells when evaluated in non-tumorigenic MCF-10A breast cells. The immunofluorescence staining of MCF-7 cells confirmed that the compounds targeted tubulin and induced multinucleation, which is a recognised sign of mitotic catastrophe. Computational docking studies of compounds 19e, 21l, and 24 in the colchicine binding site of tubulin indicated potential binding conformations for the compounds. Compounds 19e and 21l were also shown to selectively inhibit aromatase. These compounds are promising candidates for development as antiproliferative, aromatase inhibitory, and microtubule-disrupting agents for breast cancer.


2013 ◽  
Vol 266 (3) ◽  
pp. 385-398 ◽  
Author(s):  
Paulo Luiz de-Sá-Júnior ◽  
Kerly Fernanda Mesquita Pasqualoto ◽  
Adilson Kleber Ferreira ◽  
Maurício Temotheo Tavares ◽  
Mariana Celestina Frojuello Damião ◽  
...  

2012 ◽  
Vol 84 (3) ◽  
pp. S691-S692
Author(s):  
K. Thomas ◽  
P. Diagaradjane ◽  
A.A. Deorukhkar ◽  
D.K. Chatterjee ◽  
S. Barry ◽  
...  

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Macarena Calero ◽  
Michele Chiappi ◽  
Ana Lazaro-Carrillo ◽  
María José Rodríguez ◽  
Francisco Javier Chichón ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document