scholarly journals Impaired Ciliary Beat Frequency and Ciliogenesis Alteration during Airway Epithelial Cell Differentiation in COPD

Diagnostics ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1579
Author(s):  
Julien Ancel ◽  
Randa Belgacemi ◽  
Zania Diabasana ◽  
Jeanne-Marie Perotin ◽  
Arnaud Bonnomet ◽  
...  

Chronic obstructive pulmonary disease (COPD) is a frequent respiratory disease. However, its pathophysiology remains partially elucidated. Epithelial remodeling including alteration of the cilium is a major hallmark of COPD, but specific assessments of the cilium have been rarely investigated as a diagnostic tool in COPD. Here we explore the dysregulation of the ciliary function (ciliary beat frequency (CBF)) and differentiation (multiciliated cells formation in air-liquid interface cultures) of bronchial epithelial cells from COPD (n = 17) and non-COPD patients (n = 15). CBF was decreased by 30% in COPD (11.15 +/− 3.37 Hz vs. 7.89 +/− 3.39 Hz, p = 0.037). Ciliary differentiation was altered during airway epithelial cell differentiation from COPD patients. While the number of multiciliated cells decreased (p < 0.005), the number of primary ciliated cells increased (p < 0.05) and primary cilia were shorter (p < 0.05). Altogether, we demonstrate that COPD can be considered as a ciliopathy through both primary non-motile cilia modifications (related to airway epithelial cell repair and remodeling) and motile cilia function impairment (associated with decrease sputum clearance and clinical respiratory symptoms). These observations encourage considering cilia-associated features in the complex COPD physiopathology and highlight the potential of cilia-derived biomarkers for diagnosis.

2012 ◽  
Vol 241 (5) ◽  
pp. 911-923 ◽  
Author(s):  
Abraham B. Roos ◽  
Tove Berg ◽  
Jenny L. Barton ◽  
Lukas Didon ◽  
Magnus Nord

1993 ◽  
Vol 191 (1) ◽  
pp. 83-88 ◽  
Author(s):  
B. Jain ◽  
I. Rubinstein ◽  
R.A. Robbins ◽  
K.L. Leise ◽  
J.H. Sisson

Sign in / Sign up

Export Citation Format

Share Document