Biorefinery-Based Approach to Exploit Mixed Cultures of Lipomyces starkeyi and Chloroidium saccharophilum for Single Cell Oil Production

The mutualistic interactions between the oleaginous yeast Lipomyces starkeyi and the green microalga Chloroidium saccharophilum in mixed cultures were investigated to exploit possible synergistic effects. In fact, microalga could act as an oxygen generator for the yeast, while the yeast could provide carbon dioxide to microalga. The behavior of the two microorganisms alone and in mixed culture was studied in two synthetic media (YEG and BBM + G) before moving on to a real model represented by the hydrolysate of Arundo donax, used as low-cost feedstock, and previously subjected to steam explosion and enzymatic hydrolysis. The overall lipid content and lipid productivity obtained in the mixed culture of YEG, BBM + G and for the hydrolysate of Arundo donax were equal to 0.064, 0.064 and 0.081 glipid·gbiomass−1 and 30.14, 35.56 and 37.22 mglipid·L−1·day−1, respectively. The mixed cultures, in all cases, proved to be the most performing compared to the individual ones. In addition, this study provided new input for the integration of Single Cell Oil (SCO) production with agro-industrial feedstock, and the fatty acid distribution mainly consisting of stearic (C18:0) and oleic acid (C18:1) allows promising applications in biofuels, cosmetics, food additives and other products of industrial interest.

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Biorefinery-Based Approach to Exploit Mixed Cultures of Lipomyces Starkeyi and Chloroidium saccharophilum for Single Cell Oil Production

10.20944/preprints202012.0760.v1 ◽

2020 ◽

Author(s):

Gaetano Zuccaro ◽

Angelo del Mondo ◽

Gabriele Pinto ◽

Antonino Pollio ◽

Antonino De Natale

Keyword(s):

Single Cell ◽

Low Cost ◽

Food Additives ◽

Fatty Acid Distribution ◽

Arundo Donax ◽

Mixed Cultures ◽

Model Systems ◽

Giant Reed ◽

Single Cell Oil ◽

Lipomyces Starkeyi

The mutualistic interactions between the oleaginous yeast Lipomyces starkeyi and the green mi-croalga Chloroidium saccharophilum in mixed cultures were investigated to exploit possible syner-gistic effects. As a matter of facts, microalga could act as an oxygen generator for the yeast, while the yeast could provide carbon dioxide to microalga. A lignocellulosic hydrolysate from steam exploded Arundo donax (Giant reed) was used as low cost feedstock. The overall lipid content and lipid productivity obtained in the mixed culture treating the hydrolysate of Arundo donax were equal to 0.081 glipid.gbiomass-1 and 37.2 mglipid.L-1.d-1, respectively. They represented promising re-sults if compared to the model systems where synthetic media were used. This study provided new input for the integration of Single Cell Oil (SCO) production with agro-industrial feedstock and the fatty acid distribution mainly consisting of stearic (C18:0) and oleic acid (C18:1) allows promising applications in biofuels, cosmetics, food additives and other products of industrial interest.

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Integrated cascade biorefinery processes for the production of single cell oil by Lipomyces starkeyi from Arundo donax L. hydrolysates

Bioresource Technology ◽

10.1016/j.biortech.2020.124635 ◽

2020 ◽

pp. 124635

Author(s):

Nicola Di Fidio ◽

Giorgio Ragaglini ◽

Federico Dragoni ◽

Claudia Antonetti ◽

Anna Maria Raspolli Galletti

Keyword(s):

Single Cell ◽

Arundo Donax ◽

Single Cell Oil ◽

Lipomyces Starkeyi ◽

Arundo Donax L

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Production of single cell oil from Lipomyces starkeyi ATCC 56304 using biorefinery by-products

Bioresource Technology ◽

10.1016/j.biortech.2015.09.018 ◽

2015 ◽

Vol 198 ◽

pp. 268-275 ◽

Cited By ~ 19

Author(s):

Kyle V. Probst ◽

Praveen V. Vadlani

Keyword(s):

Single Cell ◽

Single Cell Oil ◽

Lipomyces Starkeyi ◽

By Products

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From paper mill waste to single cell oil: Enzymatic hydrolysis to sugars and their fermentation into microbial oil by the yeast Lipomyces starkeyi

Bioresource Technology ◽

10.1016/j.biortech.2020.123790 ◽

2020 ◽

Vol 315 ◽

pp. 123790 ◽

Cited By ~ 1

Author(s):

Nicola Di Fidio ◽

Federico Dragoni ◽

Claudia Antonetti ◽

Isabella De Bari ◽

Anna Maria Raspolli Galletti ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Single Cell ◽

Paper Mill ◽

Microbial Oil ◽

Single Cell Oil ◽

Lipomyces Starkeyi ◽

Paper Mill Waste ◽

Mill Waste

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Cell-on-hydrogel platform made of agar and alginate for rapid, low-cost, multidimensional test of antimicrobial susceptibility

Lab on a Chip ◽

10.1039/c6lc00417b ◽

2016 ◽

Vol 16 (16) ◽

pp. 3130-3138 ◽

Cited By ~ 18

Author(s):

Han Sun ◽

Zhengzhi Liu ◽

Chong Hu ◽

Kangning Ren

Keyword(s):

Image Analysis ◽

Shear Flow ◽

Single Cell ◽

Antimicrobial Susceptibility ◽

Synergistic Effects ◽

Low Cost ◽

Quick Test ◽

Cell Image ◽

Cell Image Analysis

Incorporating microfluidics into plate culture, this inexpensive platform generates stable 2D gradients of drugs for testing their synergistic effects. Culturing sample on top of the device eliminates the concern of shear flow, enables convenient collection of cells, and allows quick test based on single-cell image analysis.

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Single Cell Oil Production from Undetoxified Arundo donax L. hydrolysate by Cutaneotrichosporon curvatus

Journal of Microbiology and Biotechnology ◽

10.4014/jmb.1808.08015 ◽

2019 ◽

Vol 29 (2) ◽

pp. 256-267 ◽

Cited By ~ 9

Author(s):

Nicola Di Fidio ◽

Federico Liuzzi ◽

Silvio Mastrolitti ◽

Roberto Albergo ◽

Isabella De Bari

Keyword(s):

Single Cell ◽

Oil Production ◽

Arundo Donax ◽

Single Cell Oil ◽

Arundo Donax L

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Single Cell Oil Production by Wild Type Strain Lipomyces starkeyi Y604

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/439/1/012002 ◽

2020 ◽

Vol 439 ◽

pp. 012002

Author(s):

E Agustriana ◽

A B Juanssilfero ◽

A Andriani ◽

Fahrurrozi ◽

R Pangestu ◽

...

Keyword(s):

Single Cell ◽

Type Strain ◽

Wild Type Strain ◽

Oil Production ◽

Wild Type ◽

Single Cell Oil ◽

Lipomyces Starkeyi

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Single-cell Oil Production Using Low-Cost Carbon Sources by Newly Isolated Kocuria Y205

Archives of Hygiene Sciences ◽

10.52547/archhygsci.10.2.143 ◽

2021 ◽

Vol 10 (2) ◽

pp. 143-154

Author(s):

Alireza Rasouli ◽

Seyyed Soheil Aghaei ◽

Mohsen Zargar ◽

◽

...

Keyword(s):

Single Cell ◽

Low Cost ◽

Carbon Sources ◽

Oil Production ◽

Single Cell Oil

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Effect of Plant Oils upon Lipase and Citric Acid Production inYarrowia lipolyticaYeast

Journal of Biomedicine and Biotechnology ◽

10.1155/2009/562943 ◽

2009 ◽

Vol 2009 ◽

pp. 1-7 ◽

Cited By ~ 36

Author(s):

Farshad Darvishi ◽

Iraj Nahvi ◽

Hamid Zarkesh-Esfahani ◽

Fariborz Momenbeik

Keyword(s):

Citric Acid ◽

Olive Oil ◽

Single Cell ◽

Yarrowia Lipolytica ◽

Organic Nitrogen ◽

Low Cost ◽

Single Cell Protein ◽

Cell Protein ◽

Plant Oils ◽

Single Cell Oil

The nonconventional yeastYarrowia lipolyticadegrades very efficiently hydrophobic substrates to produce organic acids, single-cell oil, lipases, and so forth. The aim of this study was to investigate the biochemical behavior and simultaneous production of valuable metabolites such as lipase, citric acid (CA), and single-cell protein (SCP) byYarrowia lipolyticaDSM 3286 grown on various plant oils as sole carbon source. Among tested plant oils, olive oil proved to be the best medium for lipase and CA production. TheY. lipolyticaDSM 3286 produced 34.6 0.1 U/mL of lipase and also CA and SCP as by-product on olive oil medium supplemented with yeast extract. Urea, as organic nitrogen, was the best nitrogen source for CA production. The results of this study suggest that the two biotechnologically valuable products, lipase and CA, could be produced simultaneously by this strain using renewable low-cost substrates such as plant oils in one procedure.

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