scholarly journals Dissecting the Regulatory Network of Leaf Premature Senescence in Maize (Zea mays L.) Using Transcriptome Analysis of ZmELS5 Mutant

Genes ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 944
Author(s):  
Chai ◽  
Guo ◽  
Shi ◽  
Li ◽  
Tang ◽  
...  

Leaf premature senescence largely determines maize (Zea mays L.) grain yield and quality. A natural recessive premature-senescence mutant was selected from the breeding population, and near-isogenic lines were constructed using Jing24 as the recurrent parent. In the near-isogenic lines, the dominant homozygous material was wild-type (WT), and the recessive material of early leaf senescence was the premature-senescence-type ZmELS5. To identify major genes and regulatory mechanisms involved in leaf senescence, a transcriptome analysis of the ZmELS5 and WT near-isogenic lines (NILs) was performed. A total of 8,796 differentially expressed transcripts were identified between ZmELS5 and WT, including 3,811 up-regulated and 4,985 down-regulated transcripts. By combining gene ontology, Kyoto Encyclopedia of Genes and Genomes, gene set, and transcription factor enrichment analyses, key differentially expressed genes were screened. The senescence regulatory network was predicted based on these key differentially expressed genes, which indicated that the senescence process is mainly regulated by bHLH, WRKY, and AP2/EREBP family transcription factors, leading to the accumulations of jasmonic acid and ethylene. This causes stress responses and reductions in the chlorophyll a/b-binding protein activity level. Then, decreased ATP synthase activity leads to increased photosystem II photodamage, ultimately leading to leaf senescence.

2020 ◽  
Vol 139 (5) ◽  
pp. 883-891
Author(s):  
Xuemei Du ◽  
Xiaoli Wang ◽  
Jie Zhang ◽  
Sihan Zhen ◽  
Yunjun Liu ◽  
...  

BMC Genomics ◽  
2013 ◽  
Vol 14 (1) ◽  
pp. 61 ◽  
Author(s):  
Giorgio Pea ◽  
Htay Htay Aung ◽  
Elisabetta Frascaroli ◽  
Pierangelo Landi ◽  
Mario Enrico Pè

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0185838 ◽  
Author(s):  
Liancheng Wu ◽  
Mingna Li ◽  
Lei Tian ◽  
Shunxi Wang ◽  
Liuji Wu ◽  
...  

Biologia ◽  
2013 ◽  
Vol 68 (2) ◽  
Author(s):  
Quan Sun ◽  
Yingfan Cai ◽  
Shengwei Li ◽  
Min Chen ◽  
Jianchuan Mo ◽  
...  

AbstractCottonseed protein is underutilized due to the presence of pigment gland containing a toxic compound called gossypol. Cotton produces gossypol and related compounds in various tissues to protect itself against microbial, insect, and rodent attacks. Understanding the mechanism of cotton pigment gland formation and regulation of gossypol biosynthesis will greatly facilitate the research efforts in developing a cotton variety with a gossypol free seed and normally glanded foliage. In this study we make use of near-isogenic lines of cotton pigment gland to screen the genes related to gland morphogenesis applying both GeneChip and suppression subtractive hybridization (SSH) methods.We identified 880 differentially expressed genes associated with gland morphogenesis in cotton by comparing transcriptome profiles of cotton from glandless and glanded near-isogenic lines using a GeneChip. Gene ontology (GO) analysis showed that 880 genes were distributed mainly among the following GO categories: cellular process (14.45%), physiological process (14.23%), catalytic activity (9.21%), metabolism (8.99%), and cell parts (5.24%). Molecular pathway analysis revealed that these differentially expressed genes were involved in 58 KEGG pathways. Differentially expressed genes were also identified and isolated using suppression subtractive hybridization (SSH) with the same near-isogenic lines. A total of 147 ESTs were identified whose expression was either up- or down-regulated. Sequencing and BLAST analysis indicated that some of these genes were novel, while others were related to energy metabolism, transcription factors, and biotic responses. 13 genes were found to be differentially expressed both in SSH and GeneChip analysis. The expression pattern of these genes was verified by real-time PCR. The gene expression profiles produced in this study provide useful information on the molecular mechanism and regulation of gland formation and the related process in cotton. Of particular interest for future study are the genes identified by both SSH and GeneChip analysis. The outcomes are helping for our understanding of the development of specialised structures such as trichomes in plant species, from an applied and basic science perspective and promoting the application in molecular breeding.


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