scholarly journals Detection of Sepsis in Platelets Using MicroRNAs and Membrane Antigens

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1877
Author(s):  
Priscilla Cristina Moura Vieira Corrêa ◽  
Débora Monteiro Carneiro ◽  
Luciana do Socorro da Silva Valente ◽  
Fabíola Marques Diogo ◽  
Leticia Martins Lamarão ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Platelet Activation ◽  
Inverse Relationship ◽  
Diagnostic Parameters ◽  
Negative Results ◽  
Blood Banks ◽  
Membrane Antigens ◽  
And Function

The present study proposes to legitimize in sepsis a characteristic found in platelets that suffer storage lesions in blood banks, which is the increased expression of miRNA miR-320a in relation to miR-127. Under physiologically normal conditions, an inverse relationship is observed. The aim of this study was to verify whether the analysis of miR-320a and miR-127 expression in platelets could detect a decrease in their viability and function due to the presence of pathogens in the blood of patients hospitalized in the Intensive Care Unit. We also investigated the expression of membrane antigens sensitive to platelet activation. Of the 200 patients analyzed, only those who developed sepsis (140) were found to have a higher relative quantity of miR-320a than that of miR-127. This characteristic and the increased expression of membrane antigens P2Y12, CD62P, CD41, and CD61 showed a significant association (p < 0.01) with all types of sepsis evaluated in this study. Additionally, 40% of patients hospitalized for sepsis had negative results for the first cultures. We conclude that analysis of miR-127 and miR-320a expression combined with membrane antigens evaluation, in association with the available clinical and diagnostic parameters, are important tools to detect the onset of sepsis.

scholarly journals Lung ultrasound presentation of COVID-19 patients: phenotypes and correlations

2021 ◽  
Author(s):  
Gianmarco Secco ◽  
◽  
Marzia Delorenzo ◽  
Francesco Salinaro ◽  
Caterina Zattera ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Lung Ultrasound ◽  
Structure And Function ◽  
General Ward ◽  
Prognostic Role ◽  
Interstitial Syndrome ◽  
Lung Structure ◽  
And Function ◽  
The Relationship

AbstractBedside lung ultrasound (LUS) can play a role in the setting of the SarsCoV2 pneumonia pandemic. To evaluate the clinical and LUS features of COVID-19 in the ED and their potential prognostic role, a cohort of laboratory-confirmed COVID-19 patients underwent LUS upon admission in the ED. LUS score was derived from 12 fields. A prevalent LUS pattern was assigned depending on the presence of interstitial syndrome only (Interstitial Pattern), or evidence of subpleural consolidations in at least two fields (Consolidation Pattern). The endpoint was 30-day mortality. The relationship between hemogasanalysis parameters and LUS score was also evaluated. Out of 312 patients, only 36 (11.5%) did not present lung involvment, as defined by LUS score < 1. The majority of patients were admitted either in a general ward (53.8%) or in intensive care unit (9.6%), whereas 106 patients (33.9%) were discharged from the ED. In-hospital mortality was 25.3%, and 30-day survival was 67.6%. A LUS score > 13 had a 77.2% sensitivity and a 71.5% specificity (AUC 0.814; p < 0.001) in predicting mortality. LUS alterations were more frequent (64%) in the posterior lower fields. LUS score was related with P/F (R2 0.68; p < 0.0001) and P/F at FiO2 = 21% (R2 0.59; p < 0.0001). The correlation between LUS score and P/F was not influenced by the prevalent ultrasound pattern. LUS represents an effective tool in both defining diagnosis and stratifying prognosis of COVID-19 pneumonia. The correlation between LUS and hemogasanalysis parameters underscores its role in evaluating lung structure and function.

scholarly journals Sparing of muscle mass and function by passive loading in an experimental intensive care unit model

2013 ◽  
Vol 591 (5) ◽  
pp. 1385-1402 ◽  
Author(s):  
Guillaume Renaud ◽  
Monica Llano-Diez ◽  
Barbara Ravara ◽  
Luisa Gorza ◽  
Han-Zhong Feng ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Muscle Mass ◽  
Unit Model ◽  
And Function

Comparison of outcome predictions made by physicians, by nurses, and by using the Mortality Prediction Model

2001 ◽  
Vol 10 (5) ◽  
pp. 313-319 ◽  
Author(s):  
L Copeland-Fields ◽  
T Griffin ◽  
T Jenkins ◽  
M Buckley ◽  
LC Wise
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Prediction Model ◽  
Clinical Judgment ◽  
Mortality Prediction ◽  
Survival Outcomes ◽  
Emergency Physicians ◽  
Nursing Experience ◽  
Mortality Prediction Model ◽  
And Function

BACKGROUND: Critical care nurses must collaborate with physicians, patients, and patients' families when making decisions about aggressiveness of care. However, few studies address nurses' ability to predict outcomes. OBJECTIVES: To compare predictions of survival outcomes made by nurses, by physicians, and by using the Mortality Prediction Model. METHODS: Predictions of survival and function and attitudes toward aggressiveness of care based on the predictions were recorded on questionnaires in the emergency department by emergency and intensive care unit physicians and by intensive care unit nurses at the time of admission to the unit between February and September 1995 for 235 consecutive adult nontrauma patients. Scores on the Mortality Prediction Model were calculated on admission. Data on 85 of the 235 patients were analyzed by using descriptive, chi 2, and correlational statistics. Nurses' predictions of function were compared with patients' actual outcomes 6 months after admission. RESULTS: Nurses' predictions of survival were comparable to those of emergency physicians and superior to those obtained by using the objective tool. Years of nursing experience had no relationship to attitudes toward aggressiveness of care. Nurses accurately predicted functional outcomes in 52% of the followed-up cases. Intensive care physicians were more accurate than nurses and emergency physicians in predicting survival. All predictions made by clinicians were superior to those obtained by using the model. CONCLUSIONS: Nurses can predict survival outcomes as accurately as physicians do. Greater sensitivity and specificity are necessary before clinical judgment or predictive tools can be considered as screens for determining aggressiveness of care.

scholarly journals Research Advances in the Subtype of Sepsis-Associated Thrombocytopenia

2020 ◽  
Vol 26 ◽  
pp. 107602962095946
Author(s):  
Xinghui Wu ◽  
Yue Li ◽  
Huasheng Tong
Keyword(s):  
Intensive Care Unit ◽  
Bone Marrow ◽  
Intensive Care ◽  
Platelet Activation ◽  
Recent Progress ◽  
Platelet Destruction ◽  
Platelet Production ◽  
Incidence And Mortality ◽  
Pathogen Suppression ◽  
Common Laboratory

The incidence and mortality of sepsis in the intensive care unit (ICU) are extremely high. Thrombocytopenia, one of the most common laboratory abnormalities, is correlated with prognosis in sepsis. The pathophysiology of sepsis-associated thrombocytopenia (SAT) remains unclear and may be associated with several factors such as platelet activation due to vascular injury and pathogen, suppression of bone marrow, platelet-targeted antibodies and desialylation. This review summarized all these possible mechanisms in the 3 subtypes of SAT: increased platelet consumption, reduced platelet production and increased platelet destruction. Based on the clinically available platelet parameters, the evidence for identifying SAT subtypes and the recent progress in treatments according to these subtypes are proposed to provide new prospects for the management of SAT.

Organization and Function of an Intensive Care Unit in a Children??s Hospital

1967 ◽  
Vol 47 (5) ◽  
pp. 570???574
Author(s):  
LEONARD BACHMAN ◽  
JOHN J. DOWNES ◽  
CHARLES C. RICHARDS ◽  
DANIEL COYLE ◽  
EDWARD MAY
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
And Function

Structure and function of a trauma intensive care unit

2019 ◽  
Vol 86 (5) ◽  
pp. 783-790 ◽  
Author(s):  
Christopher P. Michetti ◽  
Samir M. Fakhry ◽  
Karen Brasel ◽  
Niels D. Martin ◽  
Erik J. Teicher ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Structure And Function ◽  
And Function

scholarly journals Black, White, and Hispanic Children's Health and Function 2-13 Months After Sibling Intensive Care Unit Death

2019 ◽  
Vol 210 ◽  
pp. 184-193 ◽  
Author(s):  
JoAnne M. Youngblut ◽  
Dorothy Brooten ◽  
Teresa Del-Moral ◽  
G. Patricia Cantwell ◽  
Balagangadhar Totapally ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Children's Health ◽  
Children’S Health ◽  
And Function

scholarly journals A 5G-powered robot-assisted teleultrasound diagnostic system in an intensive care unit

Critical Care ◽  
2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Shaobo Duan ◽  
Luwen Liu ◽  
Yongqing Chen ◽  
Long Yang ◽  
Ye Zhang ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Image Quality ◽  
Vital Signs ◽  
Diagnostic System ◽  
Control Panel ◽  
Ultrasound Images ◽  
Robot Assisted ◽  
Negative Results ◽  
Intestinal Gas

Abstract Background Teleultrasound provides an effective solution to problems that arise from limited medical resources, a lack of local expertise, and scenarios where the risk of infection is high. This study aims to explore the feasibility of the application of a 5G-powered robot-assisted teleultrasound diagnostic system in an intensive care unit. Methods In this study, the robot-assisted teleultrasound diagnostic system MGIUS-R3 was used. Using 5G network technology, the doctor manipulates the robotic arm to perform teleultrasound examination. The doctor can adjust parameters via the teleultrasound control panel, and real-time transmission of audio, video and ultrasound images can facilitate simultaneous communication between both parties. All patients underwent robot-assisted teleultrasound examination and bedside ultrasound examination of the liver, gallbladder, pancreas, spleen, kidney, as well as assessment for pleural effusion and abdominal effusion. We evaluated the feasibility of the application of the robot-assisted teleultrasound diagnosis system in the intensive care unit in terms of consultation duration, image quality, and safety. We also compared diagnostic consistency and differences. Results Apart from one patient who was excluded due to severe intestinal gas interference and poor image quality, a total of 32 patients were included in this study. Every patient completed all relevant examinations. Among them, 20 patients were male; 12 were female. The average age of the patients was 61 ± 20 years. The average duration of teleultrasound diagnosis was 17 ± 7 min. Of the 32 patients, 26 had positive results, 6 had negative results, and 5 had inconsistent diagnoses. The overall diagnostic results were basically the same, and there were no differences in diagnostic levels between the two. The overall average image quality score was 4.73 points, which represented a high-quality image. After robot-assisted teleultrasound examination, no significant changes were observed in the vital signs of patients as compared to before examination, and no examination-related complications were found. Conclusion The 5G-powered robot-assisted teleultrasound diagnostic system was associated with the benefits of clear images, simple operation, relatively high levels of consistency in terms of diagnostic results, higher levels of safety, and has considerable application value in the intensive care unit.

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