scholarly journals Effect of miR-302b MicroRNA Inhibition on Chicken Primordial Germ Cell Proliferation and Apoptosis Rate

Genes ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 82
Author(s):  
Bence Lázár ◽  
Nikolett Tokodyné Szabadi ◽  
Mahek Anand ◽  
Roland Tóth ◽  
András Ecker ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Cell Biology ◽  
Primordial Germ Cell ◽  
Control Cell ◽  
Cell Number ◽  
Proliferation Rate ◽  
Slight Reduction ◽  
Apoptotic Cells ◽  
Dual Inhibition

The primordial germ cells (PGCs) are the precursors for both the oocytes and spermatogonia. Recently, a novel culture system was established for chicken PGCs, isolated from embryonic blood. The possibility of PGC long-term cultivation issues a new advance in germ cell preservation, biotechnology, and cell biology. We investigated the consequence of gga-miR-302b-5P (5P), gga-miR-302b-3P (3P) and dual inhibition (5P/3P) in two male and two female chicken PGC lines. In treated and control cell cultures, the cell number was calculated every four hours for three days by the XLS Imaging system. Comparing the cell number of control and treated lines on the first day, we found that male lines had a higher proliferation rate independently from the treatments. Compared to the untreated ones, the proliferation rate and the number of apoptotic cells were considerably reduced at gga-miR-302b-5P inhibition in all PGC lines on the third day of the cultivation. The control PGC lines showed a significantly higher proliferation rate than 3P inhibited lines on Day 3 in all PGC lines. Dual inhibition of gga-miR-302b mature miRNAs caused a slight reduction in proliferation rate, but the number of apoptotic cells increased dramatically. The information gathered by examining the factors affecting cell proliferation of PGCs can lead to new data in stem cell biology.

scholarly journals Müllerian Inhibiting Substance Is Required for Germ Cell Proliferation during Early Gonadal Differentiation in Medaka (Oryzias latipes)

Endocrinology ◽  
2007 ◽  
Vol 149 (4) ◽  
pp. 1813-1819 ◽  
Author(s):  
Eri Shiraishi ◽  
Norifumi Yoshinaga ◽  
Takeshi Miura ◽  
Hayato Yokoi ◽  
Yuko Wakamatsu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Germ Cells ◽  
Sex Differentiation ◽  
Somatic Cells ◽  
Oryzias Latipes ◽  
Cell Number ◽  
Gonadal Differentiation ◽  
Loss Of Function ◽  
Germ Cell Proliferation

Müllerian inhibiting substance (MIS) is a glycoprotein belonging to the TGF-β superfamily. In mammals, MIS is responsible for the regression of Müllerian ducts in the male fetus. However, the role of MIS in gonadal sex differentiation of teleost fish, which have no Müllerian ducts, has yet to be clarified. In the present study, we examined the expression pattern of mis and mis type 2 receptor (misr2) mRNAs and the function of MIS signaling in early gonadal differentiation in medaka (teleost, Oryzias latipes). In situ hybridization showed that both mis and misr2 mRNAs were expressed in the somatic cells surrounding the germ cells of both sexes during early sex differentiation. Loss-of-function of either MIS or MIS type II receptor (MISRII) in medaka resulted in suppression of germ cell proliferation during sex differentiation. These results were supported by cell proliferation assay using 5-bromo-2′-deoxyuridine labeling analysis. Treatment of tissue fragments containing germ cells with recombinant eel MIS significantly induced germ cell proliferation in both sexes compared with the untreated control. On the other hand, culture of tissue fragments from the MIS- or MISRII-defective embryos inhibited proliferation of germ cells in both sexes. Moreover, treatment with recombinant eel MIS in the MIS-defective embryos dose-dependently increased germ cell number in both sexes, whereas in the MISRII-defective embryos, it did not permit proliferation of germ cells. These results suggest that in medaka, MIS indirectly stimulates germ cell proliferation through MISRII, expressed in the somatic cells immediately after they reach the gonadal primordium.

scholarly journals Inhibition of primordial germ cell proliferation by the medaka male determining gene Dmrt1bY

2007 ◽  
Vol 7 (1) ◽  
pp. 99 ◽  
Author(s):  
Amaury Herpin ◽  
Detlev Schindler ◽  
Anita Kraiss ◽  
Ute Hornung ◽  
Christoph Winkler ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Germ Cell Proliferation

scholarly journals Activin and TGFβ Limit Murine Primordial Germ Cell Proliferation

1999 ◽  
Vol 207 (2) ◽  
pp. 470-475 ◽  
Author(s):  
Ami J. Richards ◽  
George C. Enders ◽  
James L. Resnick
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Germ Cell Proliferation

scholarly journals Perchlorate Exposure Reduces Primordial Germ Cell Number in Female Threespine Stickleback

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0157792 ◽  
Author(s):  
Ann M. Petersen ◽  
Nathanial C. Earp ◽  
Mandy E. Redmond ◽  
John H. Postlethwait ◽  
Frank A. von Hippel ◽  
...  
Keyword(s):  
Germ Cell ◽  
Primordial Germ Cell ◽  
Cell Number ◽  
Threespine Stickleback

Autocrine and paracrine mechanisms regulating primordial germ cell proliferation

2004 ◽  
Vol 68 (1) ◽  
pp. 5-16 ◽  
Author(s):  
Eihachiro Kawase ◽  
Koichiro Hashimoto ◽  
Roger A. Pedersen
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Primordial Germ Cell ◽  
Germ Cell Proliferation

The CDK inhibitor NtKIS1a is involved in plant development,endoreduplication and restores normal development of cyclin D3;1-overexpressing plants

2002 ◽  
Vol 115 (5) ◽  
pp. 973-982 ◽  
Author(s):  
Sophie Jasinski ◽  
Catherine Riou-Khamlichi ◽  
Odile Roche ◽  
Claudette Perennes ◽  
Catherine Bergounioux ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Plant Development ◽  
Normal Development ◽  
Control Cell ◽  
Cell Number ◽  
Cyclin D ◽  
Dependent Manner ◽  
Living Plant ◽  
Essentially Normal

Plant development requires stringent controls between cell proliferation and cell differentiation. Proliferation is positively regulated by cyclin dependent kinases (CDKs). Acting in opposition to CDKs are CDK inhibitors(CKIs). The first tobacco CKI (NtKIS1a) identified was shown to inhibit in vitro the kinase activity of CDK/cyclin complexes and to interact with CDK and D-cyclins. However, these features, which are common to other plant and animal CKIs already characterised, did not provide information about the function of NtKIS1a in plants. Thus, to gain insight into the role of NtKIS1a and especially its involvement in cell proliferation during plant development, we generated transgenic Arabidopsis thaliana plants that overexpress NtKIS1a. These plants showed reduced growth with smaller organs that contained larger cells. Moreover, these plants displayed modifications in plant morphology. These results demonstrated that plant organ size and shape,as well as organ cell number and cell size, might be controlled by modulation of the single NtKIS1a gene activity. Since in mammals, D-cyclins control cell cycle progression in a CDK-dependent manner but also play a CDK independent role by sequestering the CKIs p27Kip1 and p21Cip1, we tested the significance of cyclin D-CKI interaction within a living plant. With this aim, NtKIS1a and AtCycD3;1 were overexpressed simultaneously in plants by two different methods. Our results demonstrated that overexpression of the CKI NtKIS1a restores essentially normal development in plants overexpressing AtCycD3;1, providing the first evidence of cyclin D-CKI co-operation within the context of a living plant.

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