Viscoelastic Behavior of Embroidered Scaffolds for ACL Tissue Engineering Made of PLA and P(LA-CL) After In Vitro Degradation

A rupture of the anterior cruciate ligament (ACL) is the most common knee ligament injury. Current applied reconstruction methods have limitations in terms of graft availability and mechanical properties. A new approach could be the use of a tissue engineering construct that temporarily reflects the mechanical properties of native ligament tissues and acts as a carrier structure for cell seeding. In this study, embroidered scaffolds composed of polylactic acid (PLA) and poly(lactic-co-ε-caprolactone) (P(LA-CL)) threads were tested mechanically for their viscoelastic behavior under in vitro degradation. The relaxation behavior of both scaffold types (moco: mono-component scaffold made of PLA threads, bico: bi-component scaffold made of PLA and P(LA-CL) threads) was comparable to native lapine ACL. Most of the lapine ACL cells survived 32 days of cell culture and grew along the fibers. Cell vitality was comparable for moco and bico scaffolds. Lapine ACL cells were able to adhere to the polymer surfaces and spread along the threads throughout the scaffold. The mechanical behavior of degrading matrices with and without cells showed no significant differences. These results demonstrate the potential of embroidered scaffolds as an ACL tissue engineering approach.

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In vitro degradation and mechanical properties of polyporous CaHPO4-coated Mg–Nd–Zn–Zr alloy as potential tissue engineering scaffold

Materials Letters ◽

10.1016/j.matlet.2012.09.119 ◽

2013 ◽

Vol 100 ◽

pp. 306-308 ◽

Cited By ~ 14

Author(s):

Yi Liao ◽

Desheng Chen ◽

Jialin Niu ◽

Jian Zhang ◽

Yongping Wang ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Tissue Engineering Scaffold ◽

In Vitro Degradation ◽

Zr Alloy

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Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO2 Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis

Nanomaterials ◽

10.3390/nano11051319 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1319

Author(s):

Muhammad Umar Aslam Khan ◽

Wafa Shamsan Al-Arjan ◽

Mona Saad Binkadem ◽

Hassan Mehboob ◽

Adnan Haider ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Guar Gum ◽

Porous Scaffolds ◽

Vitro Assay ◽

Vitro Analysis ◽

Nanocomposite Scaffolds

Bone tissue engineering is an advanced field for treatment of fractured bones to restore/regulate biological functions. Biopolymeric/bioceramic-based hybrid nanocomposite scaffolds are potential biomaterials for bone tissue because of biodegradable and biocompatible characteristics. We report synthesis of nanocomposite based on acrylic acid (AAc)/guar gum (GG), nano-hydroxyapatite (HAp NPs), titanium nanoparticles (TiO2 NPs), and optimum graphene oxide (GO) amount via free radical polymerization method. Porous scaffolds were fabricated through freeze-drying technique and coated with silver sulphadiazine. Different techniques were used to investigate functional group, crystal structural properties, morphology/elemental properties, porosity, and mechanical properties of fabricated scaffolds. Results show that increasing amount of TiO2 in combination with optimized GO has improved physicochemical and microstructural properties, mechanical properties (compressive strength (2.96 to 13.31 MPa) and Young’s modulus (39.56 to 300.81 MPa)), and porous properties (pore size (256.11 to 107.42 μm) and porosity (79.97 to 44.32%)). After 150 min, silver sulfadiazine release was found to be ~94.1%. In vitro assay of scaffolds also exhibited promising results against mouse pre-osteoblast (MC3T3-E1) cell lines. Hence, these fabricated scaffolds would be potential biomaterials for bone tissue engineering in biomedical engineering.

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