scholarly journals Heterologous and Homologous Expression of Proteins from Haloarchaea: Denitrification as Case of Study

2019 ◽  
Vol 21 (1) ◽  
pp. 82 ◽  
Author(s):  
Rosa María Martínez-Espinosa
Keyword(s):  
Salt Concentration ◽  
Life Forms ◽  
Eukaryotic Expression ◽  
High Salt ◽  
Expression Systems ◽  
Homologous Expression ◽  
Potential Applications ◽  
Molecular Machinery ◽  
Anaerobic Reduction ◽  
Eukaryotic Expression Systems

Haloarchaea (halophilic microbes belonging to the Archaea domain) are microorganisms requiring mid or even high salt concentrations to be alive. The molecular machinery of these organisms is adapted to such conditions, which are stressful for most life forms. Among their molecular adaptations, halophilic proteins are characterized by their high content of acidic amino acids (Aspartate (Asp) and glumate (Glu)), being only stable in solutions containing high salt concentration (between 1 and 4 M total salt concentration). Recent knowledge about haloarchaeal peptides, proteins, and enzymes have revealed that many haloarchaeal species produce proteins of interest due to their potential applications in biotechnology-based industries. Although proteins of interest are usually overproduced in recombinant prokaryotic or eukaryotic expression systems, these procedures do not accurately work for halophilic proteins, mainly if such proteins contain metallocofactors in their structures. This work summarizes the main challenges of heterologous and homologous expression of enzymes from haloarchaea, paying special attention to the metalloenzymes involved in the pathway of denitrification (anaerobic reduction of nitrate to dinitrogen), a pathway with significant implications in wastewater treatment, climate change, and biosensor design.

scholarly journals Using Eukaryotic Expression Systems to Generate Human α1,3-Fucosyltransferases That Effectively Create Selectin-Binding Glycans on Stem Cells

Biochemistry ◽  
2020 ◽  
Vol 59 (39) ◽  
pp. 3757-3771
Author(s):  
Asma S. Al-Amoodi ◽  
Kosuke Sakashita ◽  
Amal J. Ali ◽  
Ruoyu Zhou ◽  
Jae Man Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Eukaryotic Expression ◽  
Expression Systems ◽  
Selectin Binding ◽  
Eukaryotic Expression Systems

scholarly journals Production of the R2 subunit of ribonucleotide reductase from herpes simplex virus with prokaryotic and eukaryotic expression systems: higher activity of R2 produced by eukaryotic cells related to higher iron-binding capacity

1996 ◽  
Vol 320 (1) ◽  
pp. 129-135 ◽  
Author(s):  
Nathalie LAMARCHE ◽  
Gilles MATTON ◽  
Bernard MASSIE ◽  
Marc FONTECAVE ◽  
Mohamed ATTA ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Ribonucleotide Reductase ◽  
Binding Capacity ◽  
Eukaryotic Expression ◽  
Eukaryotic Cells ◽  
Expression Systems ◽  
Simplex Virus ◽  
R2 Subunit ◽  
Eukaryotic Expression Systems

The R2 subunit of ribonucleotide reductase from herpes simplex virus type 2 was overproduced with prokaryotic and eukaryotic expression systems. The recombinant R2 purified by a two-step procedure exhibited a 3-fold higher activity when produced in eukaryotic cells. Precise quantification of the R2 concentration at each step of the purification indicated that the activity was not altered during the purification procedure. Moreover, we have observed that the level of R2 expression, in eukaryotic cells as well as in prokaryotic cells, did not influence R2 activity. Extensive characterization of the recombinant R2 purified from eukaryotic and prokaryotic expression systems has shown that both types of pure R2 preparations were similar in their 76 kDa dimer contents (more than 95%) and in their ability to bind the R1 subunit. However, we have found that the higher activity of R2 produced in eukaryotic cells is more probably related to a higher capability of binding the iron cofactor as well as a 3-fold greater ability to generate the tyrosyl free radical.

Eukaryotic Expression Systems: A Comparison

1996 ◽  
Vol 8 (3) ◽  
pp. 271-282 ◽  
Author(s):  
Sabine Geisse ◽  
Hermann Gram ◽  
Beate Kleuser ◽  
Hans P. Kocher
Keyword(s):  
Eukaryotic Expression ◽  
Expression Systems ◽  
Eukaryotic Expression Systems

scholarly journals A Brief Reminder of Systems of Production and Chromatography-Based Recovery of Recombinant Protein Biopharmaceuticals

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
B. Owczarek ◽  
A. Gerszberg ◽  
K. Hnatuszko-Konka
Keyword(s):  
Recombinant Protein ◽  
Recombinant Proteins ◽  
Eukaryotic Expression ◽  
Expression Systems ◽  
Expression And Purification ◽  
Advantages And Disadvantages ◽  
Protein Molecules ◽  
The Face ◽  
Mature Field ◽  
Eukaryotic Expression Systems

Recombinant proteins are produced for various applications in laboratory and industrial settings. Among them, therapeutic applications have evolved into a mature field in recent years, affecting the face of contemporary medical treatment. This, in turn, has stimulated an ever-greater need for innovative technologies for the description, expression, and purification of recombinant protein biopharmaceuticals. Therefore, many biopharmaceuticals are synthesized in heterologous systems to obtain satisfactory yields that cannot be provided by natural sources. As more than 35 years has passed since the first recombinant biopharmaceutical (human insulin) successfully completed clinical trials in humans, we provide a brief review of the available prokaryotic and eukaryotic expression systems, listing the advantages and disadvantages of their use. Some examples of therapeutic proteins expressed in heterologous hosts are also provided. Moreover, technologies for the universal extraction of protein molecules are mentioned here, as is the methodology of their purification.

Sign in / Sign up

Export Citation Format

Share Document