scholarly journals Cross-Reactivity of Palladium in a Murine Model of Metal-induced Allergic Contact Dermatitis

2020 ◽  
Vol 21 (11) ◽  
pp. 4061
Author(s):  
Hiroaki Shigematsu ◽  
Kenichi Kumagai ◽  
Motoaki Suzuki ◽  
Takanori Eguchi ◽  
Ryota Matsubara ◽  
...  

Metal allergy is usually diagnosed by patch testing, however, the results do not necessarily reflect the clinical symptoms because of cross-reactivity between different metals. In this study, we established the novel mouse model of cross-reactive metal allergy, and aimed to elucidate the immune response in terms of T-cell receptor repertoire. This model was classified into two groups: the sensitization to nickel and challenge with palladium group, and the sensitization to chromium and challenge with palladium group. This model developed spongiotic edema with intra- and peri-epithelial infiltration of CD4+ T cells in the inflamed skin that resembles human contact dermatitis. Using T cell receptor analysis, we detected a high proportion of T cells bearing Trav8d-1-Traj49 and Trav5-1-Traj37 in the Ni- and Cr-sensitized Pd-challenged mice. Furthermore, mucosal-associated invariant T cells and invariant natural killer T cells were also detected. Our results indicated that T cells bearing Trav8d-1-Traj49 and Trav5-1-Traj37 induced the development of palladium-cross reactive allergy, and that mucosal-associated invariant T and invariant natural killer T cells were also involved in the cross-reactivity between different metals.

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153347 ◽  
Author(s):  
Nyambayar Dashtsoodol ◽  
Tomokuni Shigeura ◽  
Ritsuko Ozawa ◽  
Michishige Harada ◽  
Satoshi Kojo ◽  
...  

2011 ◽  
Vol 79 (8) ◽  
pp. 3141-3148 ◽  
Author(s):  
Sadiye Amcaoglu Rieder ◽  
Prakash Nagarkatti ◽  
Mitzi Nagarkatti

ABSTRACTThere are two important mechanisms of activation of invariant natural killer T cells (iNKT cells) by microbes: direct activation of the invariant T-cell receptor (TCR) by microbial glycolipids presented by CD1d and indirect activation, mediated by the responses of antigen-presenting cells to microbes. In this study, we provide evidence for a novel CD1d-independent direct activation of iNKT cells involving a microbial protein superantigen presented in the context of major histocompatibility complex class II (MHC-II), which plays a critical role in pathogenesis, thereby redefining the role of iNKT cells. Intranasal exposure to staphylococcal enterotoxin B (SEB) in C57BL/6 wild-type mice caused acute lung injury (ALI) characterized by vascular leak, cytokine storm, and infiltration of mononuclear cells in the lungs. In contrast, the vascular leak and inflammation were decreased by ∼50% in NKT cell-deficient Jα18−/−and CD1d−/−mice following SEB exposure, which was reversed following adoptive transfer of iNKT cells into CD1d−/−mice.In vitro, SEB could directly stimulate iNKT cells in a CD1d-independent manner via MHC-II/TCR interaction, specifically involving Vβ8. These studies not only demonstrate that iNKT cells can be activated directly by a bacterial protein superantigen independent of CD1d but also indicate that in addition to the conventional T cells, iNKT cells play a critical role in SEB-mediated ALI.


2006 ◽  
Vol 354 (11) ◽  
pp. 1117-1129 ◽  
Author(s):  
Omid Akbari ◽  
John L. Faul ◽  
Elisabeth G. Hoyte ◽  
Gerald J. Berry ◽  
Jan Wahlström ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document