scholarly journals Cell Sheets from Adipose Tissue MSC Induce Healing of Pressure Ulcer and Prevent Fibrosis via Trigger Effects on Granulation Tissue Growth and Vascularization

2020 ◽  
Vol 21 (15) ◽  
pp. 5567
Author(s):  
Natalya Alexandrushkina ◽  
Peter Nimiritsky ◽  
Roman Eremichev ◽  
Vladimir Popov ◽  
Mikhail Arbatskiy ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Pressure Ulcer ◽  
Granulation Tissue ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Tissue Growth ◽  
Matrix Remodeling ◽  
Cell Sheets ◽  
Metabolism Enzymes ◽  
Vessel Maturation ◽  
Skin Healing

We report a comparative study of multipotent mesenchymal stromal cells (MSC) delivered by injection, MSC-based cell sheets (CS) or MSC secretome to induce healing of cutaneous pressure ulcer in C57Bl/6 mice. We found that transplantation of CS from adipose-derived MSC resulted in reduction of fibrosis and recovery of skin structure with its appendages (hair and cutaneous glands). Despite short retention of CS on ulcer surface (3–7 days) it induced profound changes in granulation tissue (GT) structure, increasing its thickness and altering vascularization pattern with reduced blood vessel density and increased maturation of blood vessels. Comparable effects on GT vascularization were induced by MSC secretome, yet this treatment has failed to induce repair of skin with its appendages we observed in the CS group. Study of secretome components produced by MSC in monolayer or sheets revealed that CS produce more factors involved in pericyte chemotaxis and blood vessel maturation (PDGF-BB, HGF, G-CSF) but not sprouting inducer (VEGF165). Analysis of transcriptome using RNA sequencing and Gene Ontology mapping found in CS upregulation of proteins responsible for collagen binding and GT maturation as well as fatty acid metabolism enzymes known to be negative regulators of blood vessel sprouting. At the same time, downregulated transcripts were enriched by factors activating capillary growth, suggesting that in MSC sheets paracrine activity may shift towards matrix remodeling and maturation of vasculature, but not activation of blood vessel sprouting. We proposed a putative paracrine trigger mechanism potentially rendering an impact on GT vascularization and remodeling. Our results suggest that within sheets, MSC may change their functional state and spectrum of soluble factors that influence tissue repair and induce more effective skin healing inclining towards regeneration and reduced scarring.

Neuropilin regulation of angiogenesis

2014 ◽  
Vol 42 (6) ◽  
pp. 1623-1628 ◽  
Author(s):  
Anastasia Lampropoulou ◽  
Christiana Ruhrberg
Keyword(s):  
Blood Vessel ◽  
Vascular Permeability ◽  
Current Knowledge ◽  
Transmembrane Protein ◽  
Vascular Pathology ◽  
Eye Disease ◽  
Vegf Receptor ◽  
Neuropilin 1 ◽  
Vessel Growth ◽  
Vessel Maturation

Blood vessel formation during vertebrate development relies on a process called angiogenesis and is essential for organ growth and tissue viability. In addition, angiogenesis leads to pathological blood vessel growth in diseases with tissue ischaemia, such as neovascular eye disease and cancer. Neuropilin 1 (NRP1) is a transmembrane protein that serves as a receptor for the VEGF165 isoform of the vascular endothelial growth factor (VEGF) to enhance cell migration during angiogenesis via VEGF receptor 2 (VEGFR2), and it is also essential for VEGF-induced vascular permeability and arteriogenesis. In addition, NRP1 activation affects angiogenesis independently of VEGF signalling by activating the intracellular kinase ABL1. NRP1 also acts as a receptor for the class 3 semaphorin (SEMA3A) to regulate vessel maturation during tumour angiogenesis and vascular permeability in eye disease. In the present paper, we review current knowledge of NRP1 regulation during angiogenesis and vascular pathology.

scholarly journals KLF2 Transcription Factor Modulates Blood Vessel Maturation through Smooth Muscle Cell Migration

2007 ◽  
Vol 283 (7) ◽  
pp. 3942-3950 ◽  
Author(s):  
Jinghai Wu ◽  
Cynthia S. Bohanan ◽  
Jon C. Neumann ◽  
Jerry B. Lingrel
Keyword(s):  
Transcription Factor ◽  
Smooth Muscle ◽  
Cell Migration ◽  
Blood Vessel ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Smooth Muscle Cell Migration ◽  
Vessel Maturation

scholarly journals The Effect of Honey on Granulating Tissue of The Mandibular Bone

1970 ◽  
Vol 1 (3) ◽  
Author(s):  
Anastasia Dessy Harsono ◽  
Gentur Sudjatmiko
Keyword(s):  
Granulation Tissue ◽  
Acute Lymphocytic Leukemia ◽  
Conventional Medicine ◽  
Tissue Growth ◽  
Lymphocytic Leukemia ◽  
Poor General Condition ◽  
Mandibular Bone ◽  
Surgical Wounds ◽  
Before And After ◽  
Ancient Civilizations

Background: For many years, ancient civilizations used honey to heal wounds. Rediscovery of honey by modern physicians its use in conventional medicine. Few detailed descriptions of honey in healing difficult surgical wounds have been previously published.Methods: We report patient, male, 16 years old, who suffered acute lymphocytic leukemia and extensive mandibular eschar and had presented half of mandible bone exposed after escharectomy. Given the of closing the defect due to poor general condition of the patient, we advocated to applicate honey dressing on mandible bone to promote granulation tissue, to prevent infection and to promote epithelialization.Results: Due to honey’s effect are anti inflammatory and antimicrobial activity, promotes debridement, reduces malodor, maintains moist wound environment, and stimulates healing, in a month, all mandible exposed had been covered by granulation tissue, followed by skin grafting.Conclusion: In this case, honey had been proven effectively to stimulate granulation tissue growth on mandible bone both before and after surgery.

scholarly journals Inhibition of granulation tissue growth and elevation of cyclic AMP level by histamine

1976 ◽  
Vol 26 ◽  
pp. 127
Author(s):  
Jun Yokoyama ◽  
Kiyomi Saeki ◽  
Hidemasa Yamasaki
Keyword(s):  
Cyclic Amp ◽  
Granulation Tissue ◽  
Tissue Growth

scholarly journals Changes in blood vessel maturation in the fibrous cap of the tumor rim

2011 ◽  
Vol 103 (3) ◽  
pp. 433-438 ◽  
Author(s):  
Hisamichi Naito ◽  
Kazuhiro Takara ◽  
Taku Wakabayashi ◽  
Hiroki Kawahara ◽  
Hiroyasu Kidoya ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Fibrous Cap ◽  
Vessel Maturation

GPR43 REGULATES SODIUM BUTYRATE-INDUCED ANGIOGENESIS AND MATRIX REMODELING

2020 ◽  
Author(s):  
Pollyana Ribeiro Castro ◽  
Lucas Felipe Fernandes Bittencourt ◽  
Sébastien Larochelle ◽  
Silvia Passos Andrade ◽  
Charles Reay Mackay ◽  
...  
Keyword(s):  
Sodium Butyrate ◽  
Granulation Tissue ◽  
Local Treatment ◽  
Dermal Fibroblasts ◽  
Matrix Remodeling ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Fibrovascular Tissue

Butyrate is a short-chain fatty acid (SCFA) derived from microbiota and is involved in a range of cell processes in a concentration-dependent manner. Low concentrations of sodium butyrate (NaBu) was shown to be proangiogenic. However, the mechanisms associated with these effects are not yet fully known. Here, we investigated the contribution of the SCFA receptor GPR43 in the proangiogenic effects of local treatment with NaBu and its effects on matrix remodeling using the sponge-induced fibrovascular tissue model in mice lacking the GPR43 gene (GPR43-KO) and the wild-type (WT). We demonstrated that NaBu (0.2 mM intraimplant) treatment enhanced the neovascularization process, blood flow, and VEGF levels in a GPR43-dependent manner in the implants. Moreover, NaBu was able to modulate matrix remodeling aspects of the granulation tissue such as proteoglycans production, collagen deposition and α-SMA expression in vivo, besides to increase TGF-b1 levels in the fibrovascular tissue, in a GPR43-dependent manner. Interestingly, NaBu directly stimulated L929 murine fibroblasts migration, and TGF-β1 and collagen production in vitro. GPR43 was found to be expressed in human dermal fibroblasts, myofibroblasts and endothelial cells. Overall, our findings evidence that the metabolite-sensing receptor GPR43 contributes to the effects of low dose of NaBu in inducing angiogenesis and matrix remodeling during granulation tissue formation. These data provide important insights for the proposition of new therapeutic approaches based on NaBu, beyond the highly explored intestinal, anti-inflammatory, and anti-cancer purposes, as a local treatment to improve tissue repair, particularly, by modulating granulation tissue components.

scholarly journals Molecular analysis of Blood Vessel Maturation

2011 ◽  
Vol 22 (6) ◽  
pp. 343-347
Author(s):  
Nobuyuki TAKAKURA
Keyword(s):  
Blood Vessel ◽  
Molecular Analysis ◽  
Vessel Maturation

Inhibition of granulation tissue growth by histamine

1976 ◽  
Vol 57 (2) ◽  
pp. 268
Author(s):  
Hal G. Bingham ◽  
i Saek
Keyword(s):  
Granulation Tissue ◽  
Tissue Growth

scholarly journals Blood Vessel Maturation and Response to Vascular-Disrupting Therapy in Single Vascular Endothelial Growth Factor-A Isoform–Producing Tumors

2008 ◽  
Vol 68 (7) ◽  
pp. 2301-2311 ◽  
Author(s):  
Gillian M. Tozer ◽  
Simon Akerman ◽  
Neil A. Cross ◽  
Paul R. Barber ◽  
Meit A. Björndahl ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Blood Vessel ◽  
Vascular Endothelial ◽  
Vessel Maturation ◽  
Endothelial Growth Factor ◽  
Vascular Disrupting

Loss of smooth muscle calponin results in impaired blood vessel maturation in the tumor?host microenvironment

2007 ◽  
Vol 98 (5) ◽  
pp. 757-763 ◽  
Author(s):  
Hisako Yamamura ◽  
Noriko Hirano ◽  
Hidenori Koyama ◽  
Yoshiki Nishizawa ◽  
Katsuhito Takahashi
Keyword(s):  
Smooth Muscle ◽  
Blood Vessel ◽  
Vessel Maturation
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