scholarly journals Supramolecular Chromatographic Separation of C60 and C70 Fullerenes: Flash Column Chromatography vs. High Pressure Liquid Chromatography

2021 ◽  
Vol 22 (11) ◽  
pp. 5726
Author(s):  
Subbareddy Mekapothula ◽  
A. D. Dinga Wonanke ◽  
Matthew A. Addicoat ◽  
David J. Boocock ◽  
John D. Wallis ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Pressure Liquid Chromatography ◽  
Column Chromatography ◽  
Stationary Phases ◽  
In Silico Studies ◽  
Flash Column Chromatography ◽  
New Stationary Phase ◽  
Fullerene Mixture

A silica-bound C-butylpyrogallol[4]arene chromatographic stationary phase was prepared and characterised by thermogravimetric analysis, scanning electron microscopy, NMR and mass spectrometry. The chromatographic performance was investigated by using C60 and C70 fullerenes in reverse phase mode via flash column and high-pressure liquid chromatography (HPLC). The resulting new stationary phase was observed to demonstrate size-selective molecular recognition as postulated from our in-silico studies. The silica-bound C-butylpyrogallol[4]arene flash and HPLC stationary phases were able to separate a C60- and C70-fullerene mixture more effectively than an RP-C18 stationary phase. The presence of toluene in the mobile phase plays a significant role in achieving symmetrical peaks in flash column chromatography.

scholarly journals A tryptophan C-methyltransferase involved in streptonigrin biosynthesis in Streptomyces flocculus

1984 ◽  
Vol 220 (1) ◽  
pp. 309-313 ◽  
Author(s):  
D L Hartley ◽  
M K Speedie
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Stationary Phase ◽  
High Pressure Liquid Chromatography ◽  
Absolute Configuration ◽  
Specific Activity ◽  
Shake Culture ◽  
Methyl Group ◽  
The Absolute

A C-methyltransferase that catalyses the transfer of a methyl group from S-adenosylmethionine to C-3 of tryptophan, resulting in beta-methyltryptophan, has been identified in cell-free extracts of streptonigrin-producing Streptomyces flocculus. The absolute configuration of the product was shown to be (2S,3R)-beta-methyltryptophan by high-pressure liquid chromatography and reactivity with D- and L-amino acid oxidases. In shake culture, maximum specific activity occurs after S. flocculus enters stationary phase, but before significant streptonigrin accumulates.

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