A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network

We surveyed urine microbiota of females diagnosed with interstitial cystitis/bladder pain syndrome (IC/BPS) and matched control participants enrolled in the National Institutes of Health (NIH) Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network using the culture-independent methodology. Midstream urine specimens were analyzed with the Plex-ID molecular diagnostic platform that utilizes polymerase chain reaction–electrospray ionization–time-of-flight–mass spectrometry (PCR-ESI-TOF MS) to provide a comprehensive identification of bacterial and select fungal species. IC/BPS and control participants were evaluated for differences (presence, diversity, and abundance) in species and genus. Urine specimens obtained from 181 female IC/BPS and 182 female control participants detected a total of 92 species (41 genera). Mean (SD) species count was 2.49 (1.48) and 2.30 (1.28) among IC/BPS and control participants, respectively. Overall species composition did not significantly differ between IC/BPS and control participants at any level (p = 0.726 species level, p = 0.222 genus level). IC/BPS participants urine trended to an overabundance of Lactobacillus gasseri (p = 0.09) detected but had a lower prevalence of Corynebacterium compared with control participants (p = 0.002). The relative abundance data analysis mirrored the prevalence data differences with no significant differences in most species or genus abundance other than Lactobacillus gasseri and Corynebacterium (p = 0.08 and p = 0.001, respectively). No cause and/or effect conclusion can be drawn from this observation, but it suggests that a more comprehensive evaluation (vaginal, bowel, catheterized bladder and/or tissue-based specimens) of the lower urinary tract microbiota in IC/BPS patients is warranted.

Download Full-text

Re: A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network

The Journal of Urology ◽

10.1097/01.ju.0000614912.86465.ab ◽

2020 ◽

Vol 203 (2) ◽

pp. 258-259

Author(s):

Alan J. Wein

Keyword(s):

Interstitial Cystitis ◽

Pain Syndrome ◽

Bladder Pain Syndrome ◽

Research Network ◽

Bladder Pain ◽

Independent Analysis ◽

Culture Independent

Download Full-text

Management of Symptom Flares and Patient-reported Flare Triggers in Interstitial Cystitis/Bladder Pain Syndrome (IC/BPS)—Findings From One Site of the MAPP Research Network

Urology ◽

10.1016/j.urology.2019.01.012 ◽

2019 ◽

Vol 126 ◽

pp. 24-33 ◽

Cited By ~ 3

Author(s):

H. Henry Lai ◽

Joel Vetter ◽

Joseph Song ◽

Gerald L. Andriole ◽

Graham A. Colditz ◽

...

Keyword(s):

Interstitial Cystitis ◽

Pain Syndrome ◽

Bladder Pain Syndrome ◽

Research Network ◽

Bladder Pain ◽

Patient Reported

Download Full-text

Toll-like Receptor 4 and comorbid pain in Interstitial Cystitis/Bladder Pain Syndrome: A Multidisciplinary Approach to the Study of Chronic Pelvic Pain research network study

Brain Behavior and Immunity ◽

10.1016/j.bbi.2015.03.003 ◽

2015 ◽

Vol 49 ◽

pp. 66-74 ◽

Cited By ~ 33

Author(s):

Andrew Schrepf ◽

Catherine S. Bradley ◽

Michael O’Donnell ◽

Yi Luo ◽

Steven E. Harte ◽

...

Keyword(s):

Interstitial Cystitis ◽

Pelvic Pain ◽

Chronic Pelvic Pain ◽

Multidisciplinary Approach ◽

Pain Syndrome ◽

Bladder Pain Syndrome ◽

Research Network ◽

Toll Like Receptor ◽

Toll Like Receptor 4 ◽

Bladder Pain

Download Full-text

Dysregulation of bladder corticotropin-releasing hormone receptor in the pathogenesis of human interstitial cystitis/bladder pain syndrome

Scientific Reports ◽

10.1038/s41598-019-55584-y ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Jia-Fong Jhang ◽

Lori A. Birder ◽

Yuan-Hong Jiang ◽

Yung-Hsiang Hsu ◽

Han-Chen Ho ◽

...

Keyword(s):

Interstitial Cystitis ◽

Hormone Receptor ◽

Bladder Wall ◽

Pain Syndrome ◽

Bladder Pain Syndrome ◽

Corticotropin Releasing Hormone ◽

Bladder Pain ◽

Releasing Hormone ◽

Control Subjects ◽

And Control

AbstractStress is associated with exacerbated symptoms in patients with interstitial cystitis/bladder pain syndrome (IC/BPS). To investigate the mechanism of stress implicated on IC/BPS, we investigated expression of stress-response receptor corticotropin-releasing hormone receptor (CRHR) in bladder from IC/BPS patients. Twenty-three IC/BPS patients with Hunner’s lesion (HIC), 51 IC/BPS patients without Hunner’s lesion (NHIC), and 24 patients with stress urinary incontinence as controls were enrolled. Cystoscopic biopsies of bladder wall including mucosa and submucosa were obtained from all patients. Western blotting was used to investigate the bladder expression of the CRHR1 and CRHR2. Immunochemical staining revealed CRHR1 expression was mainly located in the submucosa while CRHR2 expression was mainly in uroepithelial cells. Compared to control subjects, the CRHR1 expression was significantly higher, while CRHR2 expression was significantly lower in IC/BPS patients. Further analysis of patients with HIC, NHIC, and control subjects showed that bladder in patients with HIC had significantly higher expressions of CRHR1 and significantly lower CRHR2. CRHR2 expression was significantly negatively correlated with O’Leary-Sant score and bladder pain. Our results indicate dysregulation of bladder CRHR1 and CRHR2 in patients with IC/BPS, and suggest CRH signaling may be associated with IC/BPS symptoms.

Download Full-text