scholarly journals Biomaterials for Cell-Surface Engineering and Their Efficacy

2021 ◽  
Vol 12 (3) ◽  
pp. 41
Author(s):  
Seoyoung Jang ◽  
Jin Gil Jeong ◽  
Tong In Oh ◽  
EunAh Lee
Keyword(s):  
Stem Cells ◽  
Cell Surface ◽  
Cell Survival ◽  
Cell Suspension ◽  
Single Cell ◽  
Surface Engineering ◽  
Single Cell Suspension ◽  
Cell Surface Engineering ◽  
Regenerative Activity

Literature in the field of stem cell therapy indicates that, when stem cells in a state of single-cell suspension are injected systemically, they show poor in vivo survival, while such cells show robust cell survival and regeneration activity when transplanted in the state of being attached on a biomaterial surface. Although an attachment-deprived state induces anoikis, when cell-surface engineering technology was adopted for stem cells in a single-cell suspension state, cell survival and regenerative activity dramatically improved. The biochemical signal coming from ECM (extracellular matrix) molecules activates the cell survival signal transduction pathway and prevents anoikis. According to the target disease, various therapeutic cells can be engineered to improve their survival and regenerative activity, and there are several types of biomaterials available for cell-surface engineering. In this review, biomaterial types and application strategies for cell-surface engineering are presented along with their expected efficacy.

scholarly journals Patterning of Growth Factor Gradients in Embryoid Bodies via Extracellular Matrix-Assisted Glycocalyx Engineering

2020 ◽  
Author(s):  
Matthew R. Naticchia ◽  
Logan K. Laubach ◽  
honigfort Daniel J. ◽  
purcell Sean C. ◽  
Kamil Godula
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Growth Factor ◽  
Cell Surface ◽  
Surface Engineering ◽  
Embryoid Bodies ◽  
Cell Surface Engineering

Cell surface engineering with synthetic glycomimetic co-receptors for FGF2 was used to establish gradients of stem cells with enhanced FGF2 affinity in embryoid bodies (EBs). Gradient shape was controlled by pre-assembly of glycomimetics into nanoscale vesicles with tunable dimensions and EB penetrance. <br>

Spatially Controlled Glycocalyx Engineering for Growth Factor Patterning in Embryoid Bodies

2020 ◽  
Author(s):  
Matthew R. Naticchia ◽  
Logan K. Laubach ◽  
honigfort Daniel J. ◽  
purcell Sean C. ◽  
Kamil Godula
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Cell Surface ◽  
Surface Engineering ◽  
Embryoid Bodies ◽  
Cell Surface Engineering

Cell surface engineering with synthetic glycomimetic co-receptors for FGF2 was used to establish gradients of stem cells with enhanced FGF2 affinity in embryoid bodies (EBs). Gradient shape was controlled by pre-assembly of glycomimetics into nanoscale vesicles with tunable dimensions and EB penetrance. <br>

Cell Surface Engineering of Mesenchymal Stem Cells

2011 ◽  
pp. 505-523 ◽  
Author(s):  
Debanjan Sarkar ◽  
Weian Zhao ◽  
Ashish Gupta ◽  
Wei Li Loh ◽  
Rohit Karnik ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Surface Engineering ◽  
Cell Surface Engineering

Apoptosis of mouse embryonic stem cells induced by single cell suspension

2000 ◽  
Vol 32 (1) ◽  
pp. 66-70 ◽  
Author(s):  
R. Koyanagi-Katsuta ◽  
N. Akimitsu ◽  
N. Arimitsu ◽  
T. Hatano ◽  
K. Sekimizu
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Suspension ◽  
Single Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Single Cell Suspension

scholarly journals Spatially Controlled Glycocalyx Engineering for Growth Factor Patterning in Embryoid Bodies

2020 ◽  
Author(s):  
Matthew R. Naticchia ◽  
Logan K. Laubach ◽  
honigfort Daniel J. ◽  
purcell Sean C. ◽  
Kamil Godula
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Cell Surface ◽  
Surface Engineering ◽  
Embryoid Bodies ◽  
Cell Surface Engineering

Cell surface engineering with synthetic glycomimetic co-receptors for FGF2 was used to establish gradients of stem cells with enhanced FGF2 affinity in embryoid bodies (EBs). Gradient shape was controlled by pre-assembly of glycomimetics into nanoscale vesicles with tunable dimensions and EB penetrance. <br>

Electron microscopy of keratinocytes cultured on a collagen substrate used as an allograft for the treatment of chronic wounds

1992 ◽  
Vol 50 (2) ◽  
pp. 1104-1105
Author(s):  
Debby A. Jennings ◽  
Michael J. Morykwas ◽  
Louis C. Argenta
Keyword(s):  
Electron Microscopy ◽  
Cell Suspension ◽  
Single Cell ◽  
Chronic Wounds ◽  
Mechanical Stability ◽  
Uv Light ◽  
Type I ◽  
Single Cell Suspension ◽  
Collagen Substrate ◽  
Dermal Collagen

Grafts of cultured allogenic or autogenic keratlnocytes have proven to be an effective treatment of chronic wounds and burns. This study utilized a collagen substrate for keratinocyte and fibroblast attachment. The substrate provided mechanical stability and augmented graft manipulation onto the wound bed. Graft integrity was confirmed by light and transmission electron microscopy.Bovine Type I dermal collagen sheets (100 μm thick) were crosslinked with 254 nm UV light (13.5 Joules/cm2) to improve mechanical properties and reduce degradation. A single cell suspension of third passage neonatal foreskin fibroblasts were plated onto the collagen. Five days later, a single cell suspension of first passage neonatal foreskin keratinocytes were plated on the opposite side of the collagen. The grafts were cultured for one month.The grafts were fixed in phosphate buffered 4% formaldehyde/1% glutaraldehyde for 24 hours. Graft pieces were then washed in 0.13 M phosphate buffer, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Polybed 812.

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