scholarly journals Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis?

2018 ◽  
Vol 4 (3) ◽  
pp. 111 ◽  
Author(s):  
Umamaheshwari Golconda ◽  
Richard Sobonya ◽  
Stephen Klotz
Keyword(s):  
Human Tissue ◽  
Silver Stain ◽  
Reactive Protein ◽  
Host Innate Immune Response ◽  
Gastrointestinal Candidiasis ◽  
Methenamine Silver ◽  
Hematoxylin And Eosin ◽  
Methenamine Silver Stain ◽  
Hematoxylin And Eosin Stain ◽  
Amyloid P

Tissue from 13 autopsy cases with invasive gastrointestinal candidiasis was studied for the binding of the pentraxins, C-reactive protein (CRP), pentraxin 3 (PTX3), and serum amyloid P component (SAP) to fungal surfaces. Invasive candidal infection was demonstrated using a hematoxylin and eosin stain and a Gomori methenamine silver stain (GMS). Immunohistochemistry was performed with CRP and PTX3 monoclonal antibodies and did not demonstrate CRP or PTX3 bound to fungi (0 of 13 cases), although CRP was extensively deposited on human tissue. A polyclonal antibody to SAP showed that SAP was bound to fungi in 12 of 13 cases. Although all three pentraxins have been reported to bind to fungi or bacteria, only SAP was bound to filamentous and yeast forms of Candida in human tissue, as detected by immunohistochemistry. SAP was abundantly present on fungi and may have affected the host innate immune response to the invading fungi.

scholarly journals Canine Pythiosis - Isolation and Identification of Pythium Insidiosum

1989 ◽  
Vol 1 (4) ◽  
pp. 295-298 ◽  
Author(s):  
John Bentinck-Smith ◽  
Arvind A. Padhye ◽  
William R. Maslin ◽  
Carolyn Hamilton ◽  
Robert K. McDonald ◽  
...  
Keyword(s):  
Intestinal Tract ◽  
Subcutaneous Tissue ◽  
Isolation And Identification ◽  
Silver Stain ◽  
Pythium Insidiosum ◽  
Tissue Sections ◽  
Zoospore Formation ◽  
Methenamine Silver ◽  
Hematoxylin And Eosin ◽  
Methenamine Silver Stain

Pythium insidiosum was isolated from the subcutaneous tissue of a 1-year-old tan crossbreed dog and from the intestinal tract of an 18-month-old Samoyed male. Gomori's methenamine silver stain was superior to hematoxylin and eosin in demonstrating the organism in tissue sections. The agent was identified as P. insidiosum by zoospore formation in an aqueous yeast extract solution containing grass blades. Exoantigens produced in culture were shown to be identical to known P. insidiosum antigens by microimmunodiffusion.

scholarly journals Efficiency of neutral honey as a tissue fixative in histopathology

2021 ◽  
Author(s):  
Nasar Alwahaibi ◽  
Buthaina Al Dhahli ◽  
Halima Al Issaei ◽  
Loai Al Wahaibi ◽  
Shadia Al Sinawi
Keyword(s):  
Periodic Acid ◽  
Routine Laboratory ◽  
Silver Stain ◽  
Periodic Acid Schiff ◽  
Human Carcinogen ◽  
Reticulin Fibers ◽  
Weak Staining ◽  
Methenamine Silver ◽  
Hematoxylin And Eosin ◽  
Methenamine Silver Stain

AbstractIn the routine laboratory, 10% neutral buffered formalin (NBF) is the fixative of choice. However, formalin is a human carcinogen. To the best of our knowledge, neutral honey, not natural or artificial honey, has not been tested to fix histological tissues. This study aimed to examine the efficiency of neutral buffered honey and other types of honey fixatives to fix histological tissues. The most two natural common Omani honey were used as fixatives, namely Sumar and date. We tested samples of rat liver, kidney, and stomach. Nine types of fixatives were used. All tissues were treated equally. The evaluation was performed blindly by three senior biomedical scientists who work in a histopathology laboratory. Hematoxylin and eosin showed adequate staining in all groups when compared to 10% NBF. The intensity and specificity of Jones Methenamine silver stain in 10% Sumer and Date honey and 10% alcoholic Sumer honey showed similar findings of 10% NBF. The specificity and intensity of all groups for Periodic acid–Schiff were comparable with 10% neutral buffered formalin accepts for 10% Sumer honey and 10% Alcoholic Date honey. However, all honey groups showed weak staining for the reticulin fibers using Gordon and Sweets method. Vimentin showed comparable findings with 10% NBF as there were no significant differences. The findings of this study are promising. Further in depth research on honey as a possible safe substitute fixative for formalin should be conducted.

Distinctive, rapid, and easy labeling of diffuse plaques in the Alzheimer brains by a new methenamine silver stain

1990 ◽  
Vol 79 (5) ◽  
pp. 569-572 ◽  
Author(s):  
H. Yamaguchi ◽  
C. Haga ◽  
S. Hirai ◽  
Y. Nakazato ◽  
K. Kosaka
Keyword(s):  
Silver Stain ◽  
Methenamine Silver ◽  
Methenamine Silver Stain ◽  
Diffuse Plaques

scholarly journals Pneumocystis jirovecii Pneumonia in a HIV-Infected Patient with a CD4 Count Greater Than 400 Cells/μL and Atovaquone Prophylaxis

2020 ◽  
Vol 2020 ◽  
pp. 1-4
Author(s):  
Abigayle Sullivan ◽  
Theresa Lanham ◽  
Ronald Krol ◽  
Shilla Zachariah
Keyword(s):  
Highly Active Antiretroviral Therapy ◽  
Lung Biopsy ◽  
Cd4 Count ◽  
Pneumocystis Jirovecii ◽  
Pneumocystis Jirovecii Pneumonia ◽  
Silver Stain ◽  
Highly Active ◽  
History Of ◽  
Methenamine Silver ◽  
Methenamine Silver Stain

We describe a rare case of Pneumocystis jirovecii pneumonia (PCP) in a heterosexual man with a pertinent medical history of well-controlled human immunodeficiency virus (HIV) on highly active antiretroviral therapy (HAART) and PCP prophylaxis with atovaquone. The patient presented with recurrent shortness of breath, worsening malaise, and fever, following treatment for hypersensitivity pneumonitis one month prior, including a twenty-four-day course of 40 milligrams daily glucocorticoid with taper. However, transbronchial biopsies, lavage, and cytology from prior admission were inconclusive. The patient refused video-assisted thoracic surgery (VATS) at that time. Upon readmission, bronchoscopy with right VATS and lung biopsy were performed. Grocott’s methenamine silver stain of right lung biopsy was positive for Pneumocystis jirovecii. This case is a rare example of PCP in a patient with a normal CD4 count (>487 cells/μL) and a low viral load (<20 copies/mL) despite PCP prophylactic antibiotics in the setting of recent iatrogenic immunosuppression.

Immunodetection of Pneumocystis carinii in bronchoalveolar lavage specimens compared with methenamine silver stain.

1996 ◽  
Vol 34 (3) ◽  
pp. 728-730 ◽  
Author(s):  
I Lautenschlager ◽  
O Lyytikainen ◽  
L Jokipii ◽  
A Jokipii ◽  
A Maiche ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Pneumocystis Carinii ◽  
Silver Stain ◽  
Methenamine Silver ◽  
Methenamine Silver Stain

scholarly journals Equine cutaneous pythiosis: a report of four cases

2004 ◽  
Vol 34 (1) ◽  
pp. 289-292 ◽  
Author(s):  
Selwyn Arlington Headley ◽  
Homero Neves Arruda Junior
Keyword(s):  
Cutaneous Lesions ◽  
Silver Stain ◽  
Pythium Insidiosum ◽  
Methenamine Silver ◽  
Methenamine Silver Stain ◽  
Muscular Tissues

Cutaneous pythiosis is described in four horses in Northern Paraná, Brazil. All animals presented ulcerative, granulomatous, cutaneous lesions that did not invade adjacent muscular tissues. Histopathological evaluations revealed eosinophilic granulomatous reactions associated with intralesional hyphae suggestive of Pythium insidiosum observed at the margins of coagula. P. insidiosum hyphae were visualized by Gomori’s methenamine silver stain.

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