scholarly journals Efficiency of neutral honey as a tissue fixative in histopathology

2021 ◽  
Author(s):  
Nasar Alwahaibi ◽  
Buthaina Al Dhahli ◽  
Halima Al Issaei ◽  
Loai Al Wahaibi ◽  
Shadia Al Sinawi
Keyword(s):  
Periodic Acid ◽  
Routine Laboratory ◽  
Silver Stain ◽  
Periodic Acid Schiff ◽  
Human Carcinogen ◽  
Reticulin Fibers ◽  
Weak Staining ◽  
Methenamine Silver ◽  
Hematoxylin And Eosin ◽  
Methenamine Silver Stain

AbstractIn the routine laboratory, 10% neutral buffered formalin (NBF) is the fixative of choice. However, formalin is a human carcinogen. To the best of our knowledge, neutral honey, not natural or artificial honey, has not been tested to fix histological tissues. This study aimed to examine the efficiency of neutral buffered honey and other types of honey fixatives to fix histological tissues. The most two natural common Omani honey were used as fixatives, namely Sumar and date. We tested samples of rat liver, kidney, and stomach. Nine types of fixatives were used. All tissues were treated equally. The evaluation was performed blindly by three senior biomedical scientists who work in a histopathology laboratory. Hematoxylin and eosin showed adequate staining in all groups when compared to 10% NBF. The intensity and specificity of Jones Methenamine silver stain in 10% Sumer and Date honey and 10% alcoholic Sumer honey showed similar findings of 10% NBF. The specificity and intensity of all groups for Periodic acid–Schiff were comparable with 10% neutral buffered formalin accepts for 10% Sumer honey and 10% Alcoholic Date honey. However, all honey groups showed weak staining for the reticulin fibers using Gordon and Sweets method. Vimentin showed comparable findings with 10% NBF as there were no significant differences. The findings of this study are promising. Further in depth research on honey as a possible safe substitute fixative for formalin should be conducted.

A case of equine abortion caused by Encephalitozoon sp.

2007 ◽  
Vol 55 (4) ◽  
pp. 525-532 ◽  
Author(s):  
L. Szeredi ◽  
A. Pospischil ◽  
L. Dencső ◽  
A. Mathis ◽  
M. Dobos-Kovács
Keyword(s):  
Epithelial Cells ◽  
Periodic Acid ◽  
Male Fetus ◽  
Silver Stain ◽  
Periodic Acid Schiff ◽  
Large Numbers ◽  
Methenamine Silver ◽  
Methenamine Silver Stain ◽  
Fetal Organs ◽  
Equine Abortion

A Lippizan mare aborted a male fetus a few days before the expected foaling date without showing any clinical sings. Focal lympho-histiocytic hepatitis in the foal and multiplex focal lympho-histiocytic villitis accompanied by villus necroses and marked hypertrophy of chorionic epithelial cells in the arcades were observed. Elongated nucleated organisms were seen in groups in vacuoles or solitarily located in the cytoplasm of the chorionic epithelial cells. The organisms were in large numbers and often extracellularly in areas of villitis and villus necroses. They were Gram-positive, stained with haematoxylin and eosin (HE), periodic acid-Schiff (PAS) and Giemsa, weakly with Warthin-Starry silver stain but not with Gömöri’s methenamine-silver stain. By ultrastructural and immunohistochemical examinations, the organisms were identified as microsporidia belonging to the genus Encephalitozoon . No Encephalitozoon organisms were detected in the fetal organs. This is the first reported case of equine abortion induced by Encephalitozoon sp. in Europe. Although abortion induced by Encephalitozoon is rare, microsporidia should be considered a differential diagnosis for intracellular organisms observed in the chorionic epithelial cells of horses.

scholarly journals Deep learning-based transformation of H&E stained tissues into special stains

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kevin de Haan ◽  
Yijie Zhang ◽  
Jonathan E. Zuckerman ◽  
Tairan Liu ◽  
Anthony E. Sisk ◽  
...  
Keyword(s):  
Visual Inspection ◽  
Kidney Diseases ◽  
Periodic Acid ◽  
Needle Core Biopsy ◽  
Silver Stain ◽  
Periodic Acid Schiff ◽  
Tissue Sections ◽  
Biopsy Tissue ◽  
Hematoxylin And Eosin

AbstractPathology is practiced by visual inspection of histochemically stained tissue slides. While the hematoxylin and eosin (H&E) stain is most commonly used, special stains can provide additional contrast to different tissue components. Here, we demonstrate the utility of supervised learning-based computational stain transformation from H&E to special stains (Masson’s Trichrome, periodic acid-Schiff and Jones silver stain) using kidney needle core biopsy tissue sections. Based on the evaluation by three renal pathologists, followed by adjudication by a fourth pathologist, we show that the generation of virtual special stains from existing H&E images improves the diagnosis of several non-neoplastic kidney diseases, sampled from 58 unique subjects (P = 0.0095). A second study found that the quality of the computationally generated special stains was statistically equivalent to those which were histochemically stained. This stain-to-stain transformation framework can improve preliminary diagnoses when additional special stains are needed, also providing significant savings in time and cost.

scholarly journals Subcutaneous Phaeohyphomycosis of Foot in an Immunocompetent Host

2011 ◽  
Vol 3 (02) ◽  
pp. 122-124 ◽  
Author(s):  
Devesh Mishra ◽  
Maneesh Singal ◽  
Mahaveer Singh Rodha ◽  
Arulselvi Subramanian
Keyword(s):  
Histopathological Examination ◽  
Periodic Acid ◽  
Foot Ulcer ◽  
Immunocompetent Host ◽  
Periodic Acid Schiff ◽  
Yeast Form ◽  
Methenamine Silver ◽  
Hematoxylin And Eosin ◽  
Mycotic Disease ◽  
Subcutaneous Phaeohyphomycosis

ABSTRACTWe report a case of subcutaneous phaeohyphomycosis of foot, which is a mycotic disease that produces brown pigmented hyphae, pseudohyphae and yeast form in combination. The patient was immunocompetent and had injury 23 years before, and developed a non healing foot ulcer which was clinically suspected as tuberculous or carcinomatous etiology. Local wide excision was done and sent in formalin for histopathological examination. Microscopically pigmented septate, hyphae and yeast forms were identified on hematoxylin and eosin stained sections which were confirmed by special stains such as periodic acid-Schiff and Gomori’s methenamine silver stains.

scholarly journals Actinomycotic Endomyometritis Associated with a Long-Term Use of Intrauterine Device Lasting for 42 Years

2019 ◽  
Vol 62 (1) ◽  
pp. 35-38
Author(s):  
Vladimír Bartoš ◽  
Jana Doboszová ◽  
Martin Sudek
Keyword(s):  
Intrauterine Device ◽  
Periodic Acid ◽  
Total Abdominal Hysterectomy ◽  
Abdominal Hysterectomy ◽  
Microscopic Investigation ◽  
Silver Stain ◽  
Biopsy Material ◽  
Periodic Acid Schiff ◽  
Large Round ◽  
Methenamine Silver Stain

In women, pelvic actinomycosis is closely associated with prolonged use of the intrauterine devices (IUD). A 70-year old female presented with intermittent blood-stained vaginal discharge. An analysis of her history revealed, she was inserted with an IUD 42 years ago, but it has remained in situ untill now. Curettage of the uterus was done, but an IUD was firmly attached inside the cavity and there was not able to remove it. A biopsy material consisted of the large round and oval granules of filamentous and mycelium-like microorganisms. They showed strong positivity with Periodic acid–Schiff stain and Gömöri methenamine silver stain. Histopathology was consisted with uterine actinomycosis. A total abdominal hysterectomy with bilateral adnexectomy was performed. The uterus contained a retained plastic IUD. Microscopic investigation revealed a diffuse chronic active endomyometritis with sporadic Actinomycetes colonies. Wearing an IUD continuously for very long periods of time can lead to actinomycotic infection, which may manifest for many years after its application. All IUD users have to keep in mind regular gynecological check-ups to avoid the complications of a retained and “forgotten” IUD.

scholarly journals Do Pentraxins Bind to Fungi in Invasive Human Gastrointestinal Candidiasis?

2018 ◽  
Vol 4 (3) ◽  
pp. 111 ◽  
Author(s):  
Umamaheshwari Golconda ◽  
Richard Sobonya ◽  
Stephen Klotz
Keyword(s):  
Human Tissue ◽  
Silver Stain ◽  
Reactive Protein ◽  
Host Innate Immune Response ◽  
Gastrointestinal Candidiasis ◽  
Methenamine Silver ◽  
Hematoxylin And Eosin ◽  
Methenamine Silver Stain ◽  
Hematoxylin And Eosin Stain ◽  
Amyloid P

Tissue from 13 autopsy cases with invasive gastrointestinal candidiasis was studied for the binding of the pentraxins, C-reactive protein (CRP), pentraxin 3 (PTX3), and serum amyloid P component (SAP) to fungal surfaces. Invasive candidal infection was demonstrated using a hematoxylin and eosin stain and a Gomori methenamine silver stain (GMS). Immunohistochemistry was performed with CRP and PTX3 monoclonal antibodies and did not demonstrate CRP or PTX3 bound to fungi (0 of 13 cases), although CRP was extensively deposited on human tissue. A polyclonal antibody to SAP showed that SAP was bound to fungi in 12 of 13 cases. Although all three pentraxins have been reported to bind to fungi or bacteria, only SAP was bound to filamentous and yeast forms of Candida in human tissue, as detected by immunohistochemistry. SAP was abundantly present on fungi and may have affected the host innate immune response to the invading fungi.

scholarly journals Report of the First Human Case of Lobomycosis in the United States

2000 ◽  
Vol 38 (3) ◽  
pp. 1283-1285 ◽  
Author(s):  
Robert A. Burns ◽  
J. Stephen Roy ◽  
Cavan Woods ◽  
Arvind A. Padhye ◽  
David W. Warnock
Keyword(s):  
Periodic Acid ◽  
White Male ◽  
High Water ◽  
Giant Cells ◽  
The United States ◽  
Human Case ◽  
Silver Stain ◽  
Periodic Acid Schiff ◽  
Male Resident ◽  
Methenamine Silver Stain

We describe the first human case of lobomycosis caused byLacazia loboi in a 42-year-old white male resident of Georgia. The patient had traveled to Venezuela 7 years earlier, where he had planned to rappel down Angel Falls in Canaima. Although he never actually rappelled the falls, he did walk under the falls at least three times, exposing himself to the high water pressures of the falls. He noticed a small pustule with surrounding erythema developing on the skin of his right chest wall. The lesion gradually increased in size and had an appearance of a keloid. For cosmetic reasons, the patient sought medical treatment to remove the lesion. After an uncomplicated excision of the lesion, the patient recovered completely. The excised tissue was fixed in formalin for pathologic examination. Tissue sections stained by hematoxylin and eosin, periodic acid-Schiff stain, and Gomori methenamine silver stain procedures showed numerous histiocytes, multinucleated giant cells, and numerous globose or subglobose, lemon-shaped cells producing multiple blastoconidia connected by narrow tube-like connectors and catenate chains of various lengths characteristic of L. loboi.

Mycotic Infections of the Alimentary Tract of Nonhuman Primates: A Review

1982 ◽  
Vol 19 (7_suppl) ◽  
pp. 93-103 ◽  
Author(s):  
G. Migaki ◽  
R. E. Schmidt ◽  
J. D. Toft ◽  
A. F. Kaufmann
Keyword(s):  
Nonhuman Primates ◽  
Alimentary Tract ◽  
Periodic Acid ◽  
New World Monkeys ◽  
Predisposing Factor ◽  
Periodic Acid Schiff ◽  
Gross Lesions ◽  
Methenamine Silver ◽  
Hematoxylin And Eosin ◽  
Mycotic Infections

Lesions of candidiasis, mucormycosis (phycomycosis), entomophthoramycosis, geotrichosis, cryptococcosis, paracoccidioidomycosis and coccidioidomycosis have been reported in the alimentary tract of nonhuman primates. Candidiasis and mucormycosis were reported most often. Both Old and New World monkeys and great apes are susceptible; infection is rare in prosimians. Ulcers and necrosis of the mucosa of the alimentary tract are the principal gross lesions. A granulomatous inflammatory process occurs in which the fungi are visible histologically on hematoxylin and eosin (HE)-stained sections, but they are seen and characterized better when stained with periodic acid-Schiff (PAS) or Gomori methenamine silver (GMS) techniques. Cultural or immunofluorescence studies, or both, are necessary for specific identification of the fungi. Immunosuppression is suggested as a predisposing factor in certain mycotic diseases.

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