scholarly journals Spatio-Temporal Expression Pattern of Ki-67, pRB, MMP-9 and Bax in Human Secondary Palate Development

Life ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 164
Author(s):  
Tanja Šimić Bilandžija ◽  
Katarina Vukojević ◽  
Anka Ćorić ◽  
Ivna Vuković Kekez ◽  
Ivana Medvedec Mikić ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Temporal Expression ◽  
Immunohistochemical Expression ◽  
Ki 67 ◽  
Palate Development ◽  
Significant Difference ◽  
Secondary Palate ◽  
Spatio Temporal ◽  
Apoptotic Activity ◽  
Temporal Expression Pattern

We analyzed the immunohistochemical expression of Ki-67, pRb, Bax, and MMP-9 during the human secondary palate formation (7th to 12th developmental weeks (DWs). The most significant proliferation was observed in the seventh DW with 32% of Ki-67-positive cells in the epithelium, while loose ectomesenchyme condensations (lec) and loose non-condensing ectomesenchyme (lnc) had only 18 and 11%, respectively (Kruskal–Wallis, p < 0.001), and diminished afterwards. Contrarily, pRb-positive cells were mostly located in the lnc (67%), with significant difference in comparison to epithelium and lec in all investigated periods (Kruskal–Wallis, p < 0.001). Ki-67- and pRb-positive cells co-expressed occasionally in all investigated periods. MMP-9 displayed a strong expression pattern with the highest number of positive cells during the seventh DW in the epithelium, with significant difference in comparison to lec and lnc (Kruskal–Wallis, p < 0.0001). The ninth DW is particularly important for the Bax expression, especially in the epithelium (84%), in comparison to lec (58%) and lnc (47%) (Kruskal–Wallis, p < 0.001). The co-expression of Bax and MMP-9 was seen only in the epithelium during seventh and ninth DWs. Our study indicates the parallel persistence of proliferation (Ki-67, pRb) and remodeling (MMP-9) that enables growth and apoptotic activity (Bax) that enable the removal of the epithelial cells at the fusion point during secondary palate formation.

scholarly journals BEST: a web server for brain expression Spatio-temporal pattern analysis

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Liyuan Guo ◽  
Wei Lin ◽  
Yidan Zhang ◽  
Wenhan Li ◽  
Jing Wang
Keyword(s):  
Gene Expression ◽  
Expression Pattern ◽  
Temporal Pattern ◽  
Pattern Analysis ◽  
Expression Patterns ◽  
Temporal Expression ◽  
Expression Pattern Analysis ◽  
Brain Expression ◽  
Spatio Temporal ◽  
Temporal Expression Pattern

Abstract Background Dysregulated gene expression patterns have been reported in several mental disorders. Limited by the difficulty of obtaining samples, psychiatric molecular mechanism research still relies heavily on clues from genetics studies. By using reference data from brain expression studies, multiple types of comprehensive gene expression pattern analysis have been performed on psychiatric genetic results. These systems-level spatial-temporal expression pattern analyses provided evidence on specific brain regions, developmental stages and molecular pathways that are possibly involved in psychiatric pathophysiology. At present, there is no online tool for such systematic analysis, which hinders the applications of analysis by non-informatics researchers such as experimental biologists and clinical molecular biologists. Results We developed the BEST web server to support Brain Expression Spatio-Temporal pattern analysis. There are three highlighted features of BEST: 1) visualization: it generates user-friendly visual results that are easy to interpret, including heatmaps, Venn diagrams, gene co-expression networks and cluster-based Manhattan gene plots; these results illustrate the complex spatio-temporal expression patterns, including expression quantification and correlation between genes; 2) integration: it provides comprehensive human brain spatio-temporal expression patterns by integrating data from currently available databases; 3) multi-dimensionality: it analyses input genes as both a whole set and several subsets (clusters) which are enriched according to co-expression patterns, and it also presents the correlation between genetic and expression data. Conclusions To the best of our knowledge, BEST is the first data tool to support comprehensive human brain spatial-temporal expression pattern analysis. It helps to bridge disease-related genetic studies and mechanism studies, provides clues for key gene and molecular system identification, and supports the analysis of disease sensitive brain region and age stages. BEST is freely available at http://best.psych.ac.cn.

scholarly journals Spatio-Temporal Expression Pattern of Frizzled Receptors after Contusive Spinal Cord Injury in Adult Rats

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e50793 ◽  
Author(s):  
Pau Gonzalez ◽  
Carmen Maria Fernandez-Martos ◽  
Carlos Gonzalez-Fernandez ◽  
Ernest Arenas ◽  
Francisco Javier Rodriguez
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Expression Pattern ◽  
Temporal Expression ◽  
Adult Rats ◽  
Cord Injury ◽  
Frizzled Receptors ◽  
Contusive Spinal Cord Injury ◽  
Spatio Temporal ◽  
Temporal Expression Pattern

Spatio-Temporal Expression and Functional Analysis of miR-206 in Developing Orofacial Tissue

MicroRNA ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 43-60 ◽  
Author(s):  
Partha Mukhopadhyay ◽  
Irina Smolenkova ◽  
Dennis Warner ◽  
Michele M. Pisano ◽  
Robert M. Greene
Keyword(s):  
Temporal Expression ◽  
Cellular Functions ◽  
Developmentally Regulated ◽  
Altered Expression ◽  
Palate Development ◽  
Functional Assays ◽  
Spatiotemporal Expression ◽  
Secondary Palate ◽  
Spatio Temporal ◽  
Functional Analyses

Background:Development of the mammalian palate is dependent on precise, spatiotemporal expression of a panoply of genes. MicroRNAs (miRNAs), the largest family of noncoding RNAs, function as crucial modulators of cell and tissue differentiation, regulating expression of key downstream genes. </P><P> Observations: Our laboratory has previously identified several developmentally regulated miRNAs, including miR-206, during critical stages of palatal morphogenesis. The current study reports spatiotemporal distribution of miR-206 during development of the murine secondary palate (gestational days 12.5-14.5). </P><P> Result and Conclusion: Potential cellular functions and downstream gene targets of miR-206 were investigated using functional assays and expression profiling, respectively. Functional analyses highlighted potential roles of miR-206 in governing TGF&#223;- and Wnt signaling in mesenchymal cells of the developing secondary palate. In addition, altered expression of miR-206 within developing palatal tissue of TGF&#223;3-/- fetuses reinforced the premise that crosstalk between this miRNA and TGF&#223;3 is crucial for secondary palate development.

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