Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets

Nicotinic acetylcholine receptors (nAChRs) are associated with various cancers, but the relation between nAChRs and cervical cancer remains unclear. Therefore, this study investigated the differential expression of nAChR subunits in human cervical cancer cell lines (SiHa, HeLa, and CaSki) and in normal ectocervical cell lines (Ect1/E6E7) at mRNA and protein levels. Two specific nAChR subtype blockers, αO-conotoxin GeXIVA and α-conotoxin TxID, were then selected to treat different human cervical cancer cell lines with specific nAChR subtype overexpression. The results showed that α3, α9, α10, and β4 nAChR subunits were overexpressed in SiHa cells compared with that in normal cells. α9 and α10 nAChR subunits were overexpressed in CaSki cells. α*-conotoxins that targeted either α9α10 or α3β4 nAChR were able to significantly inhibit cervical cancer cell proliferation. These findings may provide a basis for new targets for cervical cancer targeted therapy.

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Knockdown of long non-coding RNA CCAT1 suppresses proliferation and EMT of human cervical cancer cell lines by down-regulating Runx2

Experimental and Molecular Pathology ◽

10.1016/j.yexmp.2020.104380 ◽

2020 ◽

Vol 113 ◽

pp. 104380 ◽

Cited By ~ 5

Author(s):

Ruiping Li ◽

Jinyu Liu ◽

Jinhong Qi

Keyword(s):

Cervical Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Cervical Cancer Cell ◽

Non Coding Rna ◽

Human Cervical Cancer Cell ◽

Long Non Coding Rna ◽

Human Cervical Cancer

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Inducement of apoptosis by cucurbitacin E, a tetracyclic triterpenes, through death receptor 5 in human cervical cancer cell lines

Cell Death Discovery ◽

10.1038/cddiscovery.2017.14 ◽

2017 ◽

Vol 3 (1) ◽

Cited By ~ 7

Author(s):

Ya-Min Cheng ◽

Ching-Ju Shen ◽

Chi-Chang Chang ◽

Cheng-Yang Chou ◽

Ching-Chou Tsai ◽

...

Keyword(s):

Cervical Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Death Receptor ◽

Cancer Cell Lines ◽

Cervical Cancer Cell ◽

Death Receptor 5 ◽

Human Cervical Cancer Cell ◽

Cucurbitacin E ◽

Human Cervical Cancer

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Su.71. MHC Class-I Related Chain B and Not Chain A is Preferentially Expressed on Human Cervical Cancer Cell Lines

Clinical Immunology ◽

10.1016/j.clim.2008.03.422 ◽

2008 ◽

Vol 127 ◽

pp. S147

Author(s):

Luis Jave-Suarez ◽

Naela Arreygue-Garcia ◽

Adriana Aguilar-Lemarrroy ◽

Miriam Jimenez-Perez ◽

Angel Arregui ◽

...

Keyword(s):

Cervical Cancer ◽

Mhc Class I ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Class I ◽

Cervical Cancer Cell ◽

Human Cervical Cancer Cell ◽

Human Cervical Cancer

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Expression of Micro-RNA-492 (MiR-492) in Human Cervical Cancer Cell Lines is Upregulated by Transfection with Wild-Type P53, Irradiation, and 5-Fluorouracil Treatment In Vitro

Medical Science Monitor ◽

10.12659/msm.911585 ◽

2018 ◽

Vol 24 ◽

pp. 7750-7758 ◽

Cited By ~ 1

Author(s):

Mei Liu ◽

Zaozao Wang ◽

Qiao Liu ◽

Hongxia Zhu ◽

Ningzhi Xu

Keyword(s):

Cervical Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Micro Rna ◽

Cervical Cancer Cell ◽

Wild Type ◽

Human Cervical Cancer Cell ◽

Human Cervical Cancer

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Hexane partition from Annona crassiflora Mart. promotes cytotoxity and apoptosis on human cervical cancer cell lines

Investigational New Drugs ◽

10.1007/s10637-018-0657-y ◽

2018 ◽

Vol 37 (4) ◽

pp. 602-615 ◽

Cited By ~ 2

Author(s):

Viviane A. O. Silva ◽

Ana Laura V. Alves ◽

Marcela N. Rosa ◽

Larissa R. V. Silva ◽

Matias E. Melendez ◽

...

Keyword(s):

Cervical Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Cervical Cancer Cell ◽

Human Cervical Cancer Cell ◽

Annona Crassiflora ◽

Human Cervical Cancer

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Ectonucleotidase expression profile and activity in human cervical cancer cell lines

Biochemistry and Cell Biology ◽

10.1139/bcb-2013-0051 ◽

2014 ◽

Vol 92 (2) ◽

pp. 95-104 ◽

Cited By ~ 9

Author(s):

Aline Beckenkamp ◽

Danielle Bertodo Santana ◽

Alessandra Nejar Bruno ◽

Luciane Noal Calil ◽

Emerson André Casali ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Cells ◽

Cell Lines ◽

Cancer Cell ◽

Adenine Nucleotide ◽

Expression Profiles ◽

Cancer Cell Lines ◽

Cervical Cancer Cell ◽

Human Cervical Cancer Cell ◽

Human Cervical Cancer

Cervical cancer is the third most frequent cancer in women worldwide. Adenine nucleotide signaling is modulated by the ectonucleotidases that act in sequence, forming an enzymatic cascade. Considering the relationship between the purinergic signaling and cancer, we studied the E-NTPDases, ecto-5′-nucleotidase, and E-NPPs in human cervical cancer cell lines and keratinocytes. We evaluated the expression profiles of these enzymes using RT-PCR and quantitative real-time PCR analysis. The activities of these enzymes were examined using ATP, ADP, AMP, and p-nitrophenyl-5′-thymidine monophosphate (p-Nph-5′-TMP) as substrate, in a colorimetric assay. The extracellular adenine nucleotide hydrolysis was estimated by HPLC analysis. The hydrolysis of all substrates exhibited a linear pattern and these activities were cation-dependent. An interesting difference in the degradation rate was observed between cervical cancer cell lines SiHa, HeLa, and C33A and normal imortalized keratinocytes, HaCaT cells. The mRNA of ecto-5′-nucleotidase, E-NTPDases 5 and 6 were detectable in all cell lines, and the dominant gene expressed was the Entpd 5 enzyme, in SiHa cell line (HPV16 positive). In accordance with this result, a higher hydrolysis activity for UDP and GDP nucleotides was observed in the supernatant of the SiHa cells. Both normal and cancer cells presented activity and mRNAs of members of the NPP family. Considering that these enzymes exert an important catalytic activity, controlling purinergic nucleotide concentrations in tumors, the presence of ectonucleotidases in cervical cancer cells can be important to regulate the levels of extracellular adenine nucleotides, limiting their effects.

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