scholarly journals Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 856
Author(s):  
Yifei Ye ◽  
Xiaofeng Luan ◽  
Lingqian Zhang ◽  
Wenjie Zhao ◽  
Jie Cheng ◽  
...  
Keyword(s):  
Electric Field ◽  
Single Cell ◽  
Real Time ◽  
Single Cells ◽  
Intracellular Delivery ◽  
Microfluidic System ◽  
Label Free ◽  
Precise Control ◽  
Cell Electroporation ◽  
Single Cell Electroporation

The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics subsystem. Here, we reported a microfluidic system for precise and rapid single-cell electroporation and simultaneous impedance monitoring in a constriction microchannel. When single cells (A549) were continuously passing through the constriction microchannel, a localized high electric field was applied on the cell membrane, which resulted in highly efficient (up to 96.6%) electroporation. During a single cell entering the constriction channel, an abrupt impedance drop was noticed and demonstrated to be correlated with the occurrence of electroporation. Besides, while the cell was moving in the constriction channel, the stabilized impedance showed the capability to quantify the electroporation extent. The correspondence of the impedance variation and electroporation was validated by the intracellular delivery of the fluorescence indicator (propidium iodide). Based on the obtained results, this system is capable of precise control of electroporation and real-time, label-free impedance assessment, providing a potential tool for intracellular delivery and other biomedical applications.

Localized, Macromolecular Transport for Thin, Adherent, Single Cells Via an Automated, Single Cell Electroporation Biomanipulator

2013 ◽  
Vol 60 (11) ◽  
pp. 3113-3123 ◽  
Author(s):  
Kelly Sakaki ◽  
Hadi Esmaeilsabzali ◽  
Shabnam Massah ◽  
Gratien G. Prefontaine ◽  
Nikolai Dechev ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Macromolecular Transport ◽  
Cell Electroporation ◽  
Single Cell Electroporation

Numerical analyses on single-cell electroporation and RNA extraction under focused electric field

2018 ◽  
Vol 2018 (0) ◽  
pp. J0530202
Author(s):  
Mahmoud N. Abdelmoez ◽  
Ryuji Yokokawa ◽  
Hidetoshi Kotera ◽  
Hirofumi Shintaku
Keyword(s):  
Electric Field ◽  
Single Cell ◽  
Rna Extraction ◽  
Numerical Analyses ◽  
Cell Electroporation ◽  
Single Cell Electroporation ◽  
Focused Electric Field

scholarly journals Advanced Biosensing towards Real-Time Imaging of Protein Secretion from Single Cells

2020 ◽  
Author(s):  
Lang Zhou ◽  
Pengyu Chen ◽  
Aleksandr Simonian
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Protein Secretion ◽  
Intercellular Communication ◽  
Single Cells ◽  
Bone Marrow Failure ◽  
Cellular Protein ◽  
Label Free ◽  
Immune Effector ◽  
Real Time Imaging

Protein secretion of cells plays a vital role in intercellular communication. The abnormality and dysfunction of cellular protein secretion are associated with various physiological disorders, such as malignant proliferation of cells, aberrant immune function, and bone marrow failure. The heterogeneity of protein secretion exists not only between varying populations of cells, but also in the same phenotype of cells. Therefore, characterization of protein secretion from single cell contributes not only to the understanding of intercellular communication in immune effector, carcinogenesis and metastasis, but also to the development and improvement of diagnosis and therapy of relative diseases. In spite of abundant highly sensitive methods that have been developed for the detection of secreted proteins, majority of them fall short in providing sufficient spatial and temporal resolution for comprehensive profiling of protein secretion from single cells. The real-time imaging techniques allow rapid acquisition and manipulation of analyte information on a 2D plane, providing high spatiotemporal resolution. Here, we summarize recent advances in real-time imaging of secretory proteins from single cell, including label-free and labelling techniques, shedding light on the development of simple yet powerful methodology for real-time imaging of single-cell protein secretion.

Dielectric passivation layer as a substratum on localized single-cell electroporation

RSC Advances ◽  
2016 ◽  
Vol 6 (13) ◽  
pp. 10979-10986 ◽  
Author(s):  
Tuhin Subhra Santra ◽  
Chih-Wei Chen ◽  
Hwan-You Chang ◽  
Fan-Gang Tseng
Keyword(s):  
Electric Field ◽  
Single Cell ◽  
Passivation Layer ◽  
Cell Electroporation ◽  
Single Cell Electroporation

Without dielectric passivation layer provide bulk electroporation, whereas with passivation layer generate an intense electric field to deliver molecules precisely into single cell, as name as localized single cell electroporation (LSCEP).

scholarly journals Simulation of single cell electroporation

2021 ◽  
Vol 271 ◽  
pp. 01033
Author(s):  
Qingmeng Liu ◽  
Lei Yang
Keyword(s):  
Electric Field ◽  
Single Cell ◽  
Transmembrane Potential ◽  
Cell Model ◽  
Membrane Conductance ◽  
Round Cell ◽  
Cell Electroporation ◽  
Single Cell Model ◽  
Single Cell Electroporation

This paper attempts to introduce the dynamics of electroporation into the single cell model. The main characteristics of the model were described. Results show the generation and rise of pores in a round cell with a radius of 50 μm exposed to 40 kV/m electric field for 1 ms were analysed, so that the transmembrane potential, the number of pores and the membrane conductance could be calculated. Finally, how the model can help explain the experiment is discussed.

Single-Cell Electroporation and Real-Time Electrical Monitoring on a Microfluidic Chip

2020 ◽  
Author(s):  
Yifei Ye ◽  
Wenjie Zhao ◽  
Jian Chen ◽  
Jie Cheng ◽  
Xinyu Wei ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Microfluidic Chip ◽  
Cell Electroporation ◽  
Single Cell Electroporation

scholarly journals Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology

Micromachines ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 107 ◽  
Author(s):  
Chen Zhu ◽  
Xi Luo ◽  
Wilfred Villariza Espulgar ◽  
Shohei Koyama ◽  
Atsushi Kumanogoh ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cells ◽  
Clinical Diagnostics ◽  
Cytokine Secretion ◽  
Localized Surface Plasmon ◽  
Single Cell Level ◽  
Label Free ◽  
Real Time Monitoring ◽  
Cell Level

Cytokine secretion researches have been a main focus of studies among the scientists in the recent decades for its outstanding contribution to clinical diagnostics. Localized surface plasmon resonance (LSPR) technology is one of the conventional methods utilized to analyze these issues, as it could provide fast, label-free and real-time monitoring of biomolecule binding events. However, numerous LSPR-based biosensors in the past are usually utilized to monitor the average performance of cell groups rather than single cells. Meanwhile, the complicated sensor structures will lead to the fabrication and economic budget problems. Thus, in this paper, we report a simple synergistic integration of the cell trapping of microwell chip and gold-capped nanopillar-structured cyclo-olefin-polymer (COP) film for single cell level Interleukin 6 (IL-6) detection. Here, in-situ cytokine secreted from the trapped cell can be directly observed and analyzed through the peak red-shift in the transmittance spectrum. The fabricated device also shows the potential to conduct the real-time monitoring which would greatly help us identify the viability and biological variation of the tested single cell.

scholarly journals Unveil early-stage nanocytotoxicity by a label-free single cell pH nanoprobe

The Analyst ◽  
2020 ◽  
Vol 145 (22) ◽  
pp. 7210-7224
Author(s):  
Qingbo Yang ◽  
Alexandre Cristea ◽  
Charles Roberts ◽  
Kun Liu ◽  
Yang Song ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Early Stage ◽  
Single Cells ◽  
Label Free ◽  
Cell Ph

The developed pH nanoprobe unveiled nanomaterial properties that previously unknown (e.g., devastating cytotoxicity) via real-time label-free monitoring on single cells.

Electrophoresis-assisted single-cell electroporation for efficient intracellular delivery

2007 ◽  
Vol 10 (1) ◽  
pp. 113-116 ◽  
Author(s):  
Cristian Ionescu-Zanetti ◽  
Andrew Blatz ◽  
Michelle Khine
Keyword(s):  
Single Cell ◽  
Intracellular Delivery ◽  
Cell Electroporation ◽  
Single Cell Electroporation
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