scholarly journals Simulation of single cell electroporation

2021 ◽  
Vol 271 ◽  
pp. 01033
Author(s):  
Qingmeng Liu ◽  
Lei Yang
Keyword(s):  
Electric Field ◽  
Single Cell ◽  
Transmembrane Potential ◽  
Cell Model ◽  
Membrane Conductance ◽  
Round Cell ◽  
Cell Electroporation ◽  
Single Cell Model ◽  
Single Cell Electroporation

This paper attempts to introduce the dynamics of electroporation into the single cell model. The main characteristics of the model were described. Results show the generation and rise of pores in a round cell with a radius of 50 μm exposed to 40 kV/m electric field for 1 ms were analysed, so that the transmembrane potential, the number of pores and the membrane conductance could be calculated. Finally, how the model can help explain the experiment is discussed.

Dielectric passivation layer as a substratum on localized single-cell electroporation

RSC Advances ◽  
2016 ◽  
Vol 6 (13) ◽  
pp. 10979-10986 ◽  
Author(s):  
Tuhin Subhra Santra ◽  
Chih-Wei Chen ◽  
Hwan-You Chang ◽  
Fan-Gang Tseng
Keyword(s):  
Electric Field ◽  
Single Cell ◽  
Passivation Layer ◽  
Cell Electroporation ◽  
Single Cell Electroporation

Without dielectric passivation layer provide bulk electroporation, whereas with passivation layer generate an intense electric field to deliver molecules precisely into single cell, as name as localized single cell electroporation (LSCEP).

scholarly journals Single-Cell Electroporation with Real-Time Impedance Assessment Using a Constriction Microchannel

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 856
Author(s):  
Yifei Ye ◽  
Xiaofeng Luan ◽  
Lingqian Zhang ◽  
Wenjie Zhao ◽  
Jie Cheng ◽  
...  
Keyword(s):  
Electric Field ◽  
Single Cell ◽  
Real Time ◽  
Single Cells ◽  
Intracellular Delivery ◽  
Microfluidic System ◽  
Label Free ◽  
Precise Control ◽  
Cell Electroporation ◽  
Single Cell Electroporation

The electroporation system can serve as a tool for the intracellular delivery of foreign cargos. However, this technique is presently limited by the inaccurate electric field applied to the single cells and lack of a real-time electroporation metrics subsystem. Here, we reported a microfluidic system for precise and rapid single-cell electroporation and simultaneous impedance monitoring in a constriction microchannel. When single cells (A549) were continuously passing through the constriction microchannel, a localized high electric field was applied on the cell membrane, which resulted in highly efficient (up to 96.6%) electroporation. During a single cell entering the constriction channel, an abrupt impedance drop was noticed and demonstrated to be correlated with the occurrence of electroporation. Besides, while the cell was moving in the constriction channel, the stabilized impedance showed the capability to quantify the electroporation extent. The correspondence of the impedance variation and electroporation was validated by the intracellular delivery of the fluorescence indicator (propidium iodide). Based on the obtained results, this system is capable of precise control of electroporation and real-time, label-free impedance assessment, providing a potential tool for intracellular delivery and other biomedical applications.

Calibration of single-cell model parameters based on membrane resistance improves the accuracy of cardiac tissue simulations

2021 ◽  
pp. 101375
Author(s):  
Elnaz Pouranbarani ◽  
Lucas Arantes Berg ◽  
Rafael Sachetto Oliveira ◽  
Rodrigo Weber dos Santos ◽  
Anders Nygren
Keyword(s):  
Single Cell ◽  
Cardiac Tissue ◽  
Cell Model ◽  
Membrane Resistance ◽  
Model Parameters ◽  
Single Cell Model

scholarly journals Direct observation of frequency modulated transcription in single cells using light activation

eLife ◽  
2013 ◽  
Vol 2 ◽  
Author(s):  
Daniel R Larson ◽  
Christoph Fritzsch ◽  
Liang Sun ◽  
Xiuhau Meng ◽  
David S Lawrence ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Molecule ◽  
Single Cell Analysis ◽  
Cell Model ◽  
Single Cells ◽  
Single Gene ◽  
Gene Activation ◽  
Light Activation ◽  
Dynamic Synthesis ◽  
Single Cell Model

Single-cell analysis has revealed that transcription is dynamic and stochastic, but tools are lacking that can determine the mechanism operating at a single gene. Here we utilize single-molecule observations of RNA in fixed and living cells to develop a single-cell model of steroid-receptor mediated gene activation. We determine that steroids drive mRNA synthesis by frequency modulation of transcription. This digital behavior in single cells gives rise to the well-known analog dose response across the population. To test this model, we developed a light-activation technology to turn on a single steroid-responsive gene and follow dynamic synthesis of RNA from the activated locus.

Localized, Macromolecular Transport for Thin, Adherent, Single Cells Via an Automated, Single Cell Electroporation Biomanipulator

2013 ◽  
Vol 60 (11) ◽  
pp. 3113-3123 ◽  
Author(s):  
Kelly Sakaki ◽  
Hadi Esmaeilsabzali ◽  
Shabnam Massah ◽  
Gratien G. Prefontaine ◽  
Nikolai Dechev ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Macromolecular Transport ◽  
Cell Electroporation ◽  
Single Cell Electroporation
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