scholarly journals Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology

Micromachines ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 107 ◽  
Author(s):  
Chen Zhu ◽  
Xi Luo ◽  
Wilfred Villariza Espulgar ◽  
Shohei Koyama ◽  
Atsushi Kumanogoh ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cells ◽  
Clinical Diagnostics ◽  
Cytokine Secretion ◽  
Localized Surface Plasmon ◽  
Single Cell Level ◽  
Label Free ◽  
Real Time Monitoring ◽  
Cell Level

Cytokine secretion researches have been a main focus of studies among the scientists in the recent decades for its outstanding contribution to clinical diagnostics. Localized surface plasmon resonance (LSPR) technology is one of the conventional methods utilized to analyze these issues, as it could provide fast, label-free and real-time monitoring of biomolecule binding events. However, numerous LSPR-based biosensors in the past are usually utilized to monitor the average performance of cell groups rather than single cells. Meanwhile, the complicated sensor structures will lead to the fabrication and economic budget problems. Thus, in this paper, we report a simple synergistic integration of the cell trapping of microwell chip and gold-capped nanopillar-structured cyclo-olefin-polymer (COP) film for single cell level Interleukin 6 (IL-6) detection. Here, in-situ cytokine secreted from the trapped cell can be directly observed and analyzed through the peak red-shift in the transmittance spectrum. The fabricated device also shows the potential to conduct the real-time monitoring which would greatly help us identify the viability and biological variation of the tested single cell.

scholarly journals Toward an understanding of immune cell sociology: real-time monitoring of cytokine secretion at the single-cell level

IUBMB Life ◽  
2012 ◽  
Vol 65 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Yoshitaka Shirasaki ◽  
Mai Yamagishi ◽  
Nanako Shimura ◽  
Atsushi Hijikata ◽  
Osamu Ohara
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Immune Cell ◽  
Cytokine Secretion ◽  
Single Cell Level ◽  
Real Time Monitoring ◽  
Cell Level

scholarly journals Method for real-time monitoring of protein degradation at the single cell level

BioTechniques ◽  
2007 ◽  
Vol 42 (4) ◽  
pp. 446-450 ◽  
Author(s):  
Lijuan Zhang ◽  
Nadya G. Gurskaya ◽  
Ekaterina M. Merzlyak ◽  
Dmitry B. Staroverov ◽  
Nikolay N. Mudrik ◽  
...  
Keyword(s):  
Protein Degradation ◽  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Real Time Monitoring ◽  
Cell Level

Controlling gas/liquid exchange using microfluidics for real-time monitoring of flagellar length in living Chlamydomonas at the single-cell level

Lab on a Chip ◽  
2012 ◽  
Vol 12 (21) ◽  
pp. 4516 ◽  
Author(s):  
Xiaoni Ai ◽  
Qionglin Liang ◽  
Minna Luo ◽  
Kai Zhang ◽  
Junmin Pan ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cell Level ◽  
Real Time Monitoring ◽  
Cell Level

Label-free single-cell isolation enabled by microfluidic impact printing and real-time cellular recognition

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yiming Wang ◽  
Xiaojie Wang ◽  
Tingrui Pan ◽  
Baoqing Li ◽  
Jia-ru Chu
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Cellular Heterogeneity ◽  
Single Cell Level ◽  
Label Free ◽  
Cell Level ◽  
Cellular Recognition ◽  
Single Cell Isolation ◽  
Low Performance ◽  
Cellular Components

Analysis of cellular components at single-cell level is important to reveal cellular heterogeneity. However, current technologies to isolate individual cells are either label-based or with low performance. Here, we present...

scholarly journals Microfluidic Platforms Designed for Morphological and Photosynthetic Investigations of Chlamydomonas reinhardtii on a Single-Cell Level

Cells ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 285
Author(s):  
Eszter Széles ◽  
Krisztina Nagy ◽  
Ágnes Ábrahám ◽  
Sándor Kovács ◽  
Anna Podmaniczki ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Single Cell ◽  
Single Cells ◽  
Fluorescence Induction ◽  
Photochemical Quenching ◽  
Support Surface ◽  
Single Cell Level ◽  
Microfluidic Platforms ◽  
Cell Level ◽  
Non Photochemical Quenching

Chlamydomonas reinhardtii is a model organism of increasing biotechnological importance, yet, the evaluation of its life cycle processes and photosynthesis on a single-cell level is largely unresolved. To facilitate the study of the relationship between morphology and photochemistry, we established microfluidics in combination with chlorophyll a fluorescence induction measurements. We developed two types of microfluidic platforms for single-cell investigations: (i) The traps of the “Tulip” device are suitable for capturing and immobilizing single cells, enabling the assessment of their photosynthesis for several hours without binding to a solid support surface. Using this “Tulip” platform, we performed high-quality non-photochemical quenching measurements and confirmed our earlier results on bulk cultures that non-photochemical quenching is higher in ascorbate-deficient mutants (Crvtc2-1) than in the wild-type. (ii) The traps of the “Pot” device were designed for capturing single cells and allowing the growth of the daughter cells within the traps. Using our most performant “Pot” device, we could demonstrate that the FV/FM parameter, an indicator of photosynthetic efficiency, varies considerably during the cell cycle. Our microfluidic devices, therefore, represent versatile platforms for the simultaneous morphological and photosynthetic investigations of C. reinhardtii on a single-cell level.

Label-Free Cancer Stem-like Cell Assay Conducted at a Single Cell Level Using Microfluidic Mechanotyping Devices

2021 ◽  
Author(s):  
Miyu Terada ◽  
Sachiko Ide ◽  
Toyohiro Naito ◽  
Niko Kimura ◽  
Michiya Matsusaki ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Level ◽  
Label Free ◽  
Cell Level ◽  
Cell Assay

scholarly journals High-resolution label-free 3D mapping of extracellular pH of single living cells

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Yanjun Zhang ◽  
Yasufumi Takahashi ◽  
Sung Pil Hong ◽  
Fengjie Liu ◽  
Joanna Bednarska ◽  
...  
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Extracellular Ph ◽  
Single Cell Level ◽  
Ph Sensing ◽  
Label Free ◽  
Cell Level ◽  
Spatiotemporal Resolution ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

AbstractDynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H+ in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.

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