scholarly journals Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray

Micromachines ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 98
Author(s):  
Ryusuke Miyata ◽  
Daisuke Tadaki ◽  
Daichi Yamaura ◽  
Shun Araki ◽  
Madoka Sato ◽  
...  
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Solvent Free ◽  
Bilayer Lipid Membranes ◽  
Related Gene ◽  
Recording Site ◽  
Channel Proteins ◽  
Free Lipid ◽  
Ion Channel Proteins ◽  
Channel Currents

The reconstitution of ion-channel proteins in artificially formed bilayer lipid membranes (BLMs) forms a well-defined system for the functional analysis of ion channels and screening of the effects of drugs that act on these proteins. To improve the efficiency of the BLM reconstitution system, we report on a microarray of stable solvent-free BLMs formed in microfabricated silicon (Si) chips, where micro-apertures with well-defined nano- and micro-tapered edges were fabricated. Sixteen micro-wells were manufactured in a chamber made of Teflon®, and the Si chips were individually embedded in the respective wells as a recording site. Typically, 11 to 16 BLMs were simultaneously formed with an average BLM number of 13.1, which corresponded to a formation probability of 82%. Parallel recordings of ion-channel activities from multiple BLMs were successfully demonstrated using the human ether-a-go-go-related gene (hERG) potassium channel, of which the relation to arrhythmic side effects following drug treatment is well recognized.

Ion channels and disease

2020 ◽  
pp. 246-255
Author(s):  
Frances Ashcroft ◽  
Paolo Tammaro
Keyword(s):  
Ion Channels ◽  
Ion Channel ◽  
Single Channel ◽  
Cell Types ◽  
Channel Structure ◽  
Channel Proteins ◽  
Channel Function ◽  
Ion Channel Proteins ◽  
Single Channel Currents ◽  
Channel Currents

Ion channels are membrane proteins that act as gated pathways for the movement of ions across cell membranes. They are found in both surface and intracellular membranes and play essential roles in the physiology of all cell types. An ever-increasing number of human diseases are now known to be caused by defects in ion channel function. To understand how ion channel defects give rise to disease, it is helpful to understand how the ion channel proteins work. This chapter therefore considers what is known of ion channel structure, explains the properties of the single ion channel, and shows how single-channel currents give rise to action potentials and synaptic potentials.

ChemInform Abstract: Synthetic Peptides as Models for Ion Channel Proteins

ChemInform ◽  
2010 ◽  
Vol 24 (32) ◽  
pp. no-no
Author(s):  
K. S. AKERFELDT ◽  
J. D. LEAR ◽  
Z. R. WASSERMAN ◽  
L. A. CHUNG ◽  
W. F. DEGRADO
Keyword(s):  
Ion Channel ◽  
Synthetic Peptides ◽  
Channel Proteins ◽  
Ion Channel Proteins

Membrane supported bilayer lipid membranes array: preparation, stability and ion-channel insertion

2002 ◽  
Vol 460 (1) ◽  
pp. 23-34 ◽  
Author(s):  
G Favero ◽  
A D’Annibale ◽  
L Campanella ◽  
R Santucci ◽  
T Ferri
Keyword(s):  
Ion Channel ◽  
Lipid Membranes ◽  
Bilayer Lipid Membranes ◽  
Bilayer Lipid ◽  
Supported Bilayer

Activity of single ion channel proteins detected with a planar microstructure

2002 ◽  
Vol 81 (25) ◽  
pp. 4865-4867 ◽  
Author(s):  
Niels Fertig ◽  
Michèle Klau ◽  
Michael George ◽  
Robert H. Blick ◽  
Jan C. Behrends
Keyword(s):  
Ion Channel ◽  
Channel Proteins ◽  
Ion Channel Proteins

scholarly journals Measuring the Spatial Arrangement of Nmj-Nachr ion Channel Proteins in the Cell Membrane

2011 ◽  
Vol 100 (3) ◽  
pp. 348a
Author(s):  
Hannah DeBerg ◽  
Nir Friedman ◽  
Cong T. Nguyen ◽  
Paul Simonson ◽  
Paul Selvin
Keyword(s):  
Cell Membrane ◽  
Ion Channel ◽  
Spatial Arrangement ◽  
Channel Proteins ◽  
Ion Channel Proteins

scholarly journals Involvement of TRPV3 and TRPM8 ion channel proteins in induction of mammalian cold-inducible proteins

2018 ◽  
Vol 495 (1) ◽  
pp. 935-940 ◽  
Author(s):  
Takanori Fujita ◽  
Yu Liu ◽  
Hiroaki Higashitsuji ◽  
Katsuhiko Itoh ◽  
Koji Shibasaki ◽  
...  
Keyword(s):  
Ion Channel ◽  
Channel Proteins ◽  
Ion Channel Proteins
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