scholarly journals Environmental Conditions Affecting GABA Production in Lactococcus lactis NCDO 2118

2021 ◽  
Vol 9 (1) ◽  
pp. 122
Author(s):  
Valérie Laroute ◽  
Roberto Mazzoli ◽  
Pascal Loubière ◽  
Enrica Pessione ◽  
Muriel Cocaign-Bousquet

GABA (γ-aminobutyric acid) production has been widely described as an adaptive response to abiotic stress, allowing bacteria to survive in harsh environments. This work aimed to clarify and understand the relationship between GABA production and bacterial growth conditions, with particular reference to osmolarity. For this purpose, Lactococcus lactis NCDO 2118, a GABA-producing strain, was grown in glucose-supplemented chemically defined medium containing 34 mM L-glutamic acid, and different concentrations of salts (chloride, sulfate or phosphate ions) or polyols (sorbitol, glycerol). Unexpectedly, our data demonstrated that GABA production was not directly related to osmolarity. Chloride ions were the most significant factor influencing GABA yield in response to acidic stress while sulfate ions did not enhance GABA production. We demonstrated that the addition of chloride ions increased the glutamic acid decarboxylase (GAD) synthesis and the expression of the gadBC genes. Finally, under fed-batch conditions in a complex medium supplemented with 0.3 M NaCl and after a pH shift to 4.6, L. lactis NCDO 2118 was able to produce up to 413 mM GABA from 441 mM L-glutamic acid after only 56 h of culture, revealing the potential of L. lactis strains for intensive production of this bioactive molecule.

Diabetes ◽  
2014 ◽  
Vol 63 (8) ◽  
pp. 2876-2887 ◽  
Author(s):  
S. Robert ◽  
C. Gysemans ◽  
T. Takiishi ◽  
H. Korf ◽  
I. Spagnuolo ◽  
...  

2007 ◽  
Vol 14 (4) ◽  
pp. 280-286 ◽  
Author(s):  
Gustavo Fenalti ◽  
Ruby H P Law ◽  
Ashley M Buckle ◽  
Christopher Langendorf ◽  
Kellie Tuck ◽  
...  

Diabetes ◽  
1993 ◽  
Vol 42 (4) ◽  
pp. 631-636 ◽  
Author(s):  
W. A. Hagopian ◽  
B. Michelsen ◽  
A. E. Karlsen ◽  
F. Larsen ◽  
A. Moody ◽  
...  

2019 ◽  
Author(s):  
Chem Int

The assessment of groundwater is essential for the estimation of suitability of water for safe use. An attempt has been made to study the groundwater of selected areas of Punjab (Sheikhupura & Sahiwal) and Sindh (Sindh, Jawar Dharki and Dharki), Pakistan. The results indicate that pH, color and odor were all within limits of WHO that is pH ranges 6.5–8.5, colorless and odorless, respectively. The high values of suspended solids were observed in the Sindh-1 and Dharki samples. Microbiologically only Sahiwal and Jawar Dharki were found fit for drinking purpose. Trace metals analysis of Sheikhupura-1 and Sindh-1 showed that values do not fall within limits of WHO for Iron. The ionic concentration analysis showed that high bicarbonate (HCO3-), ions are present in the samples of Sahiwal and Dharki; Sindh-1 and Jawar Dharki samples showed very high concentration for chloride ions, all samples were satisfactory level for sulphate (SO42-), sodium, magnesium and phosphate ions except samples of Sindh-1 and Jawar Dharki. High concentration of calcium and potassium ions was observed in samples of Sindh-1, while all other samples were found fit for drinking purposes in respect of nitrate, nitrite and ammonium ions. The high concentration of Fluoride was found only in Sheikhupura-2 samples.


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