scholarly journals Common Features and Intra-Species Variation of Cutibacterium modestum Strains, and Emended Description of the Species

2021 ◽  
Vol 9 (11) ◽  
pp. 2343
Author(s):  
Itaru Dekio ◽  
Ken-ichi Okuda ◽  
Masako Nishida ◽  
Susumu Hamada-Tsutsumi ◽  
Tomo Suzuki ◽  
...  

Cutibacterium modestum is a new species coined in 2020 as the fifth species of genus Cutibacterium, which includes Cutibacterium acnes. The species is predicted as a minor but common member of skin microbiome and includes a group tentatively named as “Propionibacterium humerusii”. The description of the species has been provided only with a single strain. To establish the characteristics of C. modestum and search for possible disease-related subtypes, we investigated the biochemical characteristics of eight live strains and performed in silico comparison of nine genomes. The common features, which included the morphology of Gram-stain positive short rods, the negativity of phenylalanine arylamidase, and several unique MALDI-TOF MS spectral peaks, were considered useful in laboratory identification. Pairwise comparisons of the genomes by in silico DNA–DNA hybridization showed similarity values of 98.1% or larger, which were far higher than the subspecies cutoff of 79–80%. The 16S rRNA gene sequences of thirteen isolates and genomes were identical. Their recA gene sequences were identical except for two strains, HM-510 (HL037PA2) and Marseille-P5998, which showed unique one-nucleotide polymorphisms. The biochemical features using API kits were slightly different among the isolates but far closer than those of the nearest other species, C. acnes and Cutibacterium namnetense. Spectra of MALDI-TOF mass spectrometry showed slight differences in the presence of m/z 10,512 (10 kD chaperonin GroS) and three other peaks, further clustering the eight isolates into three subtypes. These results indicated that these isolates did not separate to form subspecies-level clusters, but subtyping is possible by using recA gene sequences or MALDI-TOF mass spectrometry spectra. Moreover, this work has confirmed that a group “P. humerusii” is included in C. modestum.

2019 ◽  
Vol 69 (4) ◽  
pp. 1087-1092 ◽  
Author(s):  
Itaru Dekio ◽  
Andrew McDowell ◽  
Mitsuo Sakamoto ◽  
Shuta Tomida ◽  
Moriya Ohkuma

In 2016, division of the genus Propionibacterium into four distinct genera was proposed. As a consequence, the species Propionibacterium acnes was transferred to Cutibacterium gen. nov. as Cutibacterium acnes comb. nov. The three recently proposed subspecies of P. acnes were not, however, accommodated in this proposal. Following a very recent validation of a new combination for C. acnes subsp. defendens and an automatically created C. acnes subsp. acnes , we now propose the new combination, C. acnes subsp. elongatum comb. nov. The type strain of Cutibacterium acnes subsp. elongatum is JCM 18919T (=NCTC 13655T). On the basis of further genomic and phenotypic (haemolysis and MALDI-TOF mass spectrometry) analyses of these subspecies, we also provide emended descriptions of the genus Cutibacterium Scholz and Kilian 2016, C. acnes subsp. acnes (Gilchrist 1900) Nouioui et al. 2018, and C. acnes subsp. defendens (McDowell et al. 2016) Nouioui et al. 2018.


2021 ◽  
pp. 103835
Author(s):  
Sébastien Bridel ◽  
Stephen C. Watts ◽  
Louise M. Judd ◽  
Taylor Harshegyi ◽  
Virginie Passet ◽  
...  

2001 ◽  
Vol 34 (7) ◽  
pp. 2389-2393 ◽  
Author(s):  
Weihua Ming ◽  
Xianwen Lou ◽  
Robert D. van de Grampel ◽  
Joost L. J. van Dongen ◽  
Rob van der Linde

2014 ◽  
Vol 226 (02) ◽  
pp. 68-71 ◽  
Author(s):  
M. Artacho-Reinoso ◽  
P. Olbrich ◽  
P. Solano-Paéz ◽  
P. Ybot-Gonzalez ◽  
J. Lepe ◽  
...  

2016 ◽  
Vol 120 ◽  
pp. 68-71 ◽  
Author(s):  
Gerald Larrouy-Maumus ◽  
Abigail Clements ◽  
Alain Filloux ◽  
Ronan R. McCarthy ◽  
Serge Mostowy

2010 ◽  
Vol 6 (11) ◽  
pp. 2214 ◽  
Author(s):  
Eleonora Cerasoli ◽  
Paulina D. Rakowska ◽  
Adrian Horgan ◽  
Jascindra Ravi ◽  
Melanie Bradley ◽  
...  

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