scholarly journals Grape Extract Promoted α-MSH-Induced Melanogenesis in B16F10 Melanoma Cells, Which Was Inverse to Resveratrol

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5959
Author(s):  
Siqi Zhou ◽  
Drira Riadh ◽  
Kazuichi Sakamoto
Keyword(s):  
Biological Activities ◽  
Melanoma Cells ◽  
Positive Control ◽  
Point Of View ◽  
Melanin Synthesis ◽  
Related Protein ◽  
B16f10 Melanoma ◽  
Grape Extract ◽  
B16f10 Melanoma Cells ◽  
Tyrosinase Related Protein

Melanin is a natural pigment produced by cells to prevent damage caused by ultraviolet radiation. Previously, resveratrol was shown to reduce melanin synthesis. As a natural polyphenol with various biological activities, resveratrol occurs in a variety of beverages and plant foods, such as grapes. Therefore, we investigated whether grape extracts containing resveratrol also had the ability to regulate melanin synthesis. In this study, we used mouse B16F10 melanoma cells as a model for melanin synthesis with the melanogenesis-inducing α-melanocyte-stimulating hormone (α-MSH) as a positive control. Our results confirmed previous reports that resveratrol reduces melanin synthesis by reducing the activity of the rate-limiting enzyme tyrosinase. In contrast, the grape extract could not reduce melanin synthesis, and in fact promoted melanogenesis in the presence of α-MSH. The expression of genes related to melanin synthesis, such as tyrosinase, tyrosinase-related protein-1, tyrosinase-related protein-2, and microphthalmia-associated transcription factor, also supports these phenomena, which means that even in the presence of resveratrol, grape extract will strengthen the function of α-MSH in promoting melanin synthesis. Therefore, these results also provide a point of view for research on cosmetics.

scholarly journals Anti-melanogenesis Constituents from the Seaweed Dictyota Coriacea

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Ryeo Kyeong Ko ◽  
Min-Chul Kang ◽  
Sang Suk Kim ◽  
Tae Heon Oh ◽  
Gi-Ok Kim ◽  
...  
Keyword(s):  
Substrate Concentration ◽  
Ethanol Extract ◽  
Melanoma Cells ◽  
Positive Control ◽  
Therapeutic Agents ◽  
Melanin Synthesis ◽  
B16f10 Melanoma ◽  
Synthesis Inhibition ◽  
Effective Components ◽  
B16f10 Melanoma Cells

This study was conducted to identify the anti-melanogenesis constituents from a seaweed Dictyota coriacea (Holmes). Three known compounds, viz. 1,9-dihydroxycrenulide (1), epiloliolide (2) and D-mannitol (3), were isolated from the ethanol extract. The melanin synthesis inhibition activities were evaluated using B16F10 melanoma cells for the isolates. Compared with the positive control, arbutin, compounds 1 and 2 exhibited more potency, showing 27.8 and 22.6 % inhibition activities at a substrate concentration of 30 μg/mL. Our studies also indicate that these compounds are not cytotoxic. Hence, they might prove to be useful therapeutic agents for treating hyperpigmentation and effective components of whitening cosmetics.

Effect of apigenin-7-glucoside, genkwanin and naringenin on tyrosinase activity and melanin synthesis in B16F10 melanoma cells

Life Sciences ◽  
2016 ◽  
Vol 144 ◽  
pp. 80-85 ◽  
Author(s):  
Nouha Nasr Bouzaiene ◽  
Fadwa Chaabane ◽  
Aicha Sassi ◽  
Leila Chekir-Ghedira ◽  
Kamel Ghedira
Keyword(s):  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Melanin Synthesis ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

scholarly journals Berberine regulates melanin synthesis by activating PI3K/AKT, ERK and GSK3β in B16F10 melanoma cells

2015 ◽  
Vol 35 (4) ◽  
pp. 1011-1016 ◽  
Author(s):  
YOUNG CHAN SONG ◽  
YONGHEE LEE ◽  
HYEONG MI KIM ◽  
MOO YEOL HYUN ◽  
YUN YOUNG LIM ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Melanin Synthesis ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

scholarly journals Flavonoid Glycosides from Ziziphus jujuba var. inermis (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis

2021 ◽  
Vol 22 (14) ◽  
pp. 7701
Author(s):  
Ilandarage Menu Neelaka Molagoda ◽  
Kyoung-Tae Lee ◽  
Athapaththu Mudiyanselage Gihan Kavinda Athapaththu ◽  
Yung-Hyun Choi ◽  
Jaeyoung Hwang ◽  
...  
Keyword(s):  
Biological Activities ◽  
Melanoma Cells ◽  
Flavonoid Glycosides ◽  
B16f10 Melanoma ◽  
Anticancer Activities ◽  
Melanocyte Stimulating Hormone ◽  
Zebrafish Larvae ◽  
B16f10 Melanoma Cells ◽  
Ziziphus Jujuba ◽  
Stimulating Hormone

Ziziphus jujuba extracts possess a broad spectrum of biological activities, such as antioxidant and anticancer activities in melanoma cancers. Nevertheless, the compounds contain high antioxidant capacities and anticancer activities in melanoma cells, shown to be effective in hyperpigmentation disorders, but whether flavonoid glycosides from Z. jujuba regulate anti-melanogenesis remains unclear. In this study, we evaluated the anti-melanogenic activity of five flavonoid glycosides from Z. jujuba var. inermis (Bunge) Rehder seeds, including jujuboside A (JUA), jujuboside B (JUB), epiceanothic acid (EPA), betulin (BTL), and 6’’’-feruloylspinosin (FRS), in B16F10 melanoma cells and zebrafish larvae. According to our results, JUB, EPA, and FRS potently inhibited α-melanocyte-stimulating hormone (α-MSH)-induced melanogenesis and prevented hyperpigmentation in zebrafish larvae. In particular, under α-MSH-stimulated conditions, FRS most significantly inhibited α-MSH-induced intracellular and extracellular melanin content in B16F10 melanoma cells. Additionally, JUB, EPS, and FRS remarkably downregulated melanogenesis in α-MSH-treated zebrafish larvae, with no significant change in heart rate. Neither JUA nor BTA were effective in downregulating melanogenesis in B16F10 melanoma cells and zebrafish larvae. Furthermore, JUB, EPA, and FRS directly inhibited in vitro mushroom tyrosinase enzyme activity. JUB, EPA, and FRS also downregulated cyclic adenosine monophosphate (cAMP) levels and the phosphorylation of cAMP-response element-binding protein (CREB), and subsequent microphthalmia transcription factor (MITF) and tyrosinase expression. In conclusion, this study demonstrated that JUB, EPA, and FRS isolated from Z. jujuba var. inermis (Bunge) Rehder seeds exhibit potent anti-melanogenic properties by inhibition of the cAMP-CERB-MITF axis and consequent tyrosinase activity.

scholarly journals Antimelanogenic activities of piperlongumine derived from Piper longum on murine B16F10 melanoma cells in vitro and zebrafish embryos in vivo: its molecular mode of depigmenting action

2019 ◽  
Vol 62 (1) ◽  
Author(s):  
Hwang-Ju Jeon ◽  
Kyeongnam Kim ◽  
Yong-Deuk Kim ◽  
Sung-Eun Lee
Keyword(s):  
Melanoma Cells ◽  
Positive Control ◽  
Zebrafish Embryos ◽  
B16f10 Melanoma ◽  
Melanin Production ◽  
Melanocyte Stimulating Hormone ◽  
B16f10 Melanoma Cells ◽  
Pharmaceutical Industries

Abstract In this study, the antimelanogenic activity of piperlongumine in murine B16F10 melanoma cells and zebrafish was investigated, and its mode of antimelanogenic action was elucidated using quantitative reverse transcription-polymerase chain reaction. A melanocyte-stimulating hormone (α-MSH, 200 nM) was used to induce melanin production in B16F10 melanoma cells, and kojic acid (200 μM) was used as a positive control. Piperlongumine had no inhibitory effects on cell growth at the treated concentrations (3 and 6 μM), and it significantly reduced total melanin production. Piperlongumine decreased the expression of Mitf, Tyr, Trp-1, and Trp-2 and tyrosinase activity was also dramatically reduced by the piper amide addition under α-MSH treatment. With these findings, zebrafish embryos were used to confirm antimelanogenic activity of piperlongumine, and it showed the potent antimelanogenic activity at the concentration of 1 μM. Altogether, piperlongumine has potent antimelanogenic activity, and these results support it as a candidate for natural depigmentation agent in a cosmetic and pharmaceutical industries.

P047 Role of IL-18 in the melanin synthesis of B16F10 melanoma cells

Cytokine ◽  
2012 ◽  
Vol 59 (3) ◽  
pp. 533-534 ◽  
Author(s):  
I.-J. Yoon ◽  
M. Park ◽  
H.-R. Choi ◽  
H.-S. Kang
Keyword(s):  
Melanoma Cells ◽  
Melanin Synthesis ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells
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