scholarly journals Antioxidant Assessment of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Cultivars of Peanut (Arachis hypogaea)

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6778
Author(s):  
Gaurav Gajurel ◽  
Rokib Hasan ◽  
Fabricio Medina-Bolivar

Peanut produces prenylated stilbenoids upon biotic stress. However, the role of these compounds against oxidative stress have not been thoroughly elucidated. To this end, the antioxidant capacity of extracts enriched in prenylated stilbenoids and derivatives was studied. To produce these extracts, hairy root cultures of peanut cultivars Hull, Tifrunner, and Georgia Green were co-treated with methyl jasmonate, cyclodextrin, hydrogen peroxide, and magnesium chloride and then the stilbenoids were extracted from the culture medium. Among the three cultivars, higher levels of the stilbenoid derivatives arachidin-1 and arachidin-6 were detected in cultivar Tifrunner. Upon reaction with 2,2-diphenyl-1picrylhydrazyl, extracts from cultivar Tifrunner showed the highest antioxidant capacity with an IC50 of 6.004 µg/mL. Furthermore, these extracts had significantly higher antioxidant capacity at 6.25 µg/mL and 3.125 µg/mL when compared to extracts from cultivars Hull and Georgia Green. The stilbenoid-rich extracts from peanut hairy roots show high antioxidant capacity and merit further study as potential nutraceuticals to promote human health.

2010 ◽  
Vol 48 (5) ◽  
pp. 310-318 ◽  
Author(s):  
Jose Condori ◽  
Ganapathy Sivakumar ◽  
John Hubstenberger ◽  
Maureen C. Dolan ◽  
Victor S. Sobolev ◽  
...  

2009 ◽  
Vol 64 (3-4) ◽  
pp. 201-209 ◽  
Author(s):  
Amani M. Marzouk

Six triterpene acids identified as betulinic, oleanolic, ursolic, 3-epimaslinic, alphitolic and euscaphic acids have been isolated from a dichloromethane extract of hairy root cultures of Ocimum basilicum L. (Lamiaceae). These cultures were obtained by genetic transformation using Agrobacterium rhizogenes. The extract as well as the isolated compounds were evaluated for their hepatoprotective activity by measuring their effect on the oxidative stress status of liver, induced by carbon tetrachloride, in albino rats and in liver homogenate in vitro. All tested compounds displayed hepatoprotective activity comparable to oleanolic and ursolic acids.


2010 ◽  
Vol 26 (5) ◽  
pp. 1344-1351 ◽  
Author(s):  
Julie A. Abbott ◽  
Fabricio Medina-Bolivar ◽  
Elizabeth M. Martin ◽  
Abigail S. Engelberth ◽  
Hector Villagarcia ◽  
...  

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 509 ◽  
Author(s):  
Lingling Fang ◽  
Tianhong Yang ◽  
Fabricio Medina-Bolivar

Prenylated stilbenoids are phenolic compounds produced in a small number of plants such as peanut (Arachis hypogaea) to counteract biotic and abiotic stresses. In addition to their role in plant defense, they exhibit biological activities with potential application in human health. Whereas non-prenylated stilbenoids such as resveratrol are commercially available, the availability of prenylated stilbenoids is limited. To this end, hairy root cultures of peanut were developed as an elicitor-controlled bioproduction platform for prenylated stilbenoids. An orthogonal array design approach led to the elucidation of an optimized elicitation procedure consisting of co-treatment of the hairy root cultures with 18 g/L methyl-β-cyclodextrin, 125 µM methyl jasmonate, 3 mM hydrogen peroxide (H2O2) and medium supplementation with additional 1 mM magnesium chloride. After 168-h of elicitor treatment, the combined yield of the prenylated stilbenoids arachidin-1, arachidin-2, arachidin-3 and arachidin-5 reached approximately 750 mg/L (equivalent to 107 mg/g DW). Moreover, hairy root cultures from the wild Arachis species A. duranensis and A. ipaensis were developed and shown to produce prenylated stilbenoids upon elicitor treatment. These wild Arachis hairy root lines may provide a platform to elucidate the biosynthetic origin of prenylated stilbenoids in peanut.


2002 ◽  
Vol 35 (1) ◽  
pp. 1 ◽  
Author(s):  
Elizabeth Agostini ◽  
Josefa Hernández-Ruiz ◽  
Marino B. Arnao ◽  
Silvia R. Milrad ◽  
Horacio A. Tigier ◽  
...  

2017 ◽  
Vol 105 ◽  
pp. 124-132 ◽  
Author(s):  
María Perassolo ◽  
Alejandra B. Cardillo ◽  
M. Laura Mugas ◽  
Susana C. Núñez Montoya ◽  
Ana M. Giulietti ◽  
...  

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