Bioassay-Guided Fractionation of Siparuna glycycarpa n-Butanol Extract with Inhibitory Activity against Influenza A(H1N1)pdm09 Virus by Centrifugal Partition Chromatography (CPC)

Siparuna glycycarpa occurs in the Amazon region, and some species of this genus are used in Brazilian folk medicine. A recent study showed the inhibitory effect of this species against influenza A(H1N1)pdm09 virus, and in order to acquire active fractions, a polar solvent system n-butanol-methanol-water (9:1:10, v/v) was selected and used for bioassay-guided fractionation of n-butanol extract by centrifugal partition chromatography (CPC). The upper phase was used as stationary phase and the lower phase as mobile (descending mode). Among the collected fractions, the ones coded SGA, SGC, SGD, and SGO showed the highest antiviral inhibition levels (above 74%) at 100 µg·mL−1 after 24 h of infection. The bioactive fractions chemical profiles were investigated by LC-HRMS/MS data in positive and negative ionization modes exploring the Global Natural Products Social Molecular Networking (GNPS) platform to build a molecular network. Benzylisoquinoline alkaloids were annotated in the fractions coded SGA, SGC, and SGD collected during elution step. Aporphine alkaloids, O-glycosylated flavonoids, and dihydrochalcones in SGO were acquired with the change of mobile phase from lower aqueous to upper organic. Benzylisoquinolinic and aporphine alkaloids as well as glycosylated flavonoids were annotated in the most bioactive fractions suggesting this group of compounds as responsible for antiviral activity.

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Bioassay-Guided Separation of Centipeda minima Using Comprehensive Linear Gradient Centrifugal Partition Chromatography

Molecules ◽

10.3390/molecules25133077 ◽

2020 ◽

Vol 25 (13) ◽

pp. 3077

Author(s):

Ji Hoon Kim ◽

Eun Ju Jung ◽

Yun Jung Lee ◽

En Mei Gao ◽

Ahmed Shah Syed ◽

...

Keyword(s):

Ethyl Acetate ◽

Gradient Elution ◽

Solvent System ◽

Luciferase Assay ◽

Partition Chromatography ◽

Centrifugal Partition Chromatography ◽

Lower Phase ◽

Linear Gradient ◽

Centipeda Minima ◽

Water Saturated

A comprehensive linear gradient solvent system for centrifugal partition chromatography (CPC) was developed for the bioassay-guided isolation of natural compounds. The gradient solvent system consisted of three different ternary biphasic solvents types: n-hexane–acetonitrile–water (10:2:8, v/v), ethyl acetate–acetonitrile–water (10:2:8, v/v), and water-saturated n-butanol–acetonitrile–water (10:2:8, v/v). The lower phase of the n-hexane–acetonitrile–water (10:2:8, v/v) was used as the stationary phase, while its upper phase, as well as ethyl acetate–acetonitrile–water (10:2:8), and water-saturated n-butanol–acetonitrile–water (10:2:8, v/v) were pumped to generate a linear gradient elution, increasing the mobile phase polarity. We used the gradient CPC to identify antioxidant response elements (AREs), inducing compounds from Centipeda minima, using an ARE-luciferase assay in HepG2 cells, which led to the purification of the active molecules 3-methoxyquercetin and brevilin A. The developed CPC solvent systems allow the separation and isolation of compounds with a wide polarity range, allowing active molecule identification in the complex crude extract of natural products.

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Separation of Aeruginosin-865 from Cultivated Soil Cyanobacterium (Nostoc sp.) by Centrifugal Partition Chromatography combined with Gel Permeation Chromatography

Natural Product Communications ◽

10.1177/1934578x1501001022 ◽

2015 ◽

Vol 10 (10) ◽

pp. 1934578X1501001

Author(s):

José Cheel ◽

Mirjana Minceva ◽

Petra Urajová ◽

Rabya Aslam ◽

Pavel Hrouzek ◽

...

Keyword(s):

Gel Permeation Chromatography ◽

Solvent System ◽

Partition Chromatography ◽

Centrifugal Partition Chromatography ◽

Lower Phase ◽

Revolution Speed ◽

Cultivated Soil ◽

Gel Permeation ◽

Isolated Compound ◽

Soil Cyanobacteria

Aeruginosin-865 was isolated from cultivated soil cyanobacteria using a combination of centrifugal partition chromatography (CPC) and gel permeation chromatography. The solubility of Aer-865 in different solvents was evaluated using the conductor-like screening model for real solvents (COSMO-RS). The CPC separation was performed in descending mode with a biphasic solvent system composed of water-n-BuOH-acetic acid (5:4:1, v/v/v). The upper phase was used as a stationary phase, whereas the lower phase was employed as a mobile phase at a flow rate of 10 mL/min. The revolution speed and temperature of the separation column were 1700 rpm and 25°C, respectively. Preparative CPC separation followed by gel permeation chromatography was performed on 50 mg of crude extract yielding Aer-865 (3.5 mg), with a purity over 95% as determined by HPLC. The chemical identity of the isolated compound was confirmed by comparing its spectroscopic data (UV, HRESI-MS, HRESI-MS/MS) with those of an authentic standard and data available in the literature.

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Seroprevalence study of infection with influenza A(H1N1)pdm09 virus in San Felipe Town, Chile

World Journal of Cardiovascular Diseases ◽

10.4236/wjcd.2013.37075 ◽

2013 ◽

Vol 03 (07) ◽

pp. 476-482

Author(s):

Olea Andrea ◽

Fasce Rodrigo ◽

Aguilera Ximena ◽

Oliva Otavio ◽

Muñoz Sergio ◽

...

Keyword(s):

Influenza A ◽

Seroprevalence Study ◽

Pdm09 Virus ◽

Influenza A H1n1

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Fluctuations in influenza-like illness epidemics and suicide mortality: A time-series regression of 13-year mortality data in South Korea

PLoS ONE ◽

10.1371/journal.pone.0244596 ◽

2021 ◽

Vol 16 (2) ◽

pp. e0244596

Author(s):

Sun Jae Jung ◽

Sung-Shil Lim ◽

Jin-Ha Yoon

Keyword(s):

Time Series ◽

South Korea ◽

Influenza A ◽

Suicide Mortality ◽

Mortality Data ◽

Time Series Regression ◽

Influenza Like Illness ◽

Pdm09 Virus ◽

Influenza A H1n1 ◽

Novel Influenza A

Aims We explored the association between influenza epidemic and suicide mortality rates in a large population using a time-series regression of 13-year mortality data in South Korea. Methods Weekly suicide mortalities and influenza-like illness (ILI) were analyzed using time series regression. Regression coefficient for suicide mortality based on percentage change of ILI was calculated using a quasi-Poisson regression. Non-linear distributed lag models with quadratic function up to 24 weeks were constructed. Results The association between ILI and suicide mortality increased significantly up to 8 weeks post-influenza diagnosis. A significant positive association between ILI and suicide mortality was observed from 2009, when a novel influenza A(H1N1)pdm09 virus provoked a worldwide pandemic. No meaningful association between these factors was observed before 2009. Conclusion There was a significant positive relationship between ILI and suicide mortality after 2009, when a novel influenza A(H1N1)pdm09 virus provoked a worldwide pandemic.

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