Bio-Mediated Synthesis of Reduced Graphene Oxide Nanoparticles from Chenopodium album: Their Antimicrobial and Anticancer Activities

A novel method of preparing reduced graphene oxide (RGOX) from graphene oxide (GOX) was developed employing vegetable extract, Chenopodium album, as a reducing and stabilizing agent. Chenopodium album is a green leafy vegetable with a low shelf life, fresh leaves of this vegetable are encouraged to be used due to high water content. The previously modified ‘Hummers method’ has been in practice for the preparation of GOX by using precursor graphite powder. In this study, green synthesis of RGOX was functionally verified by employing FTIR and UV-visible spectroscopy, along with SEM and TEM. Our results demonstrated typical morphology of RGOX stacked in layers that appeared as silky, transparent, and rippled. The antibacterial activity was shown by analyzing minimal inhibitory concentration values, agar diffusion assay, fluorescence techniques. It showed enhanced antibacterial activity against Gram-positive and Gram-negative bacteria in comparison to GOX. It has also been shown that the synthesized compound exhibited enhanced antibiofilm activity as compared to its parent compound. The efficacy of RGOX and GOX has been demonstrated on a human breast cancer cell line, which suggested RGOX as a potential anticancer agent.

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Green Synthesis of Reduced Graphene Oxide-Supported Palladium Nanoparticles by Coleus amboinicus and Its Enhanced Catalytic Efficiency and Antibacterial Activity

Crystals ◽

10.3390/cryst11020134 ◽

2021 ◽

Vol 11 (2) ◽

pp. 134

Author(s):

Koduru Mallikarjuna ◽

Lebaka Veeranjaneya Reddy ◽

Sarah Al-Rasheed ◽

Arifullah Mohammed ◽

Sreedevi Gedi ◽

...

Keyword(s):

Antibacterial Activity ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Palladium Nanoparticles ◽

Catalytic Efficiency ◽

Tem Analysis ◽

Reduced Graphene ◽

Supported Palladium ◽

Medical Disciplines ◽

Coleus Amboinicus

Novel reduced graphene oxide-supported palladium nanoparticles (RGO-PN) were synthesized under ultrasonication, a method that utilizes Coleus amboinicus as a bio-reduction agent. Green synthesized RGO-PN nanoparticles with a crystallite size in the range of 40–50 nm were confirmed in X-ray diffraction (XRD) spectra. RGO-PN show an absorption peak at 220 nm while reduced graphene oxide (RGO) shows its maximal absorbance at 210 nm. The scanning electron microscope image revealed that 40-nm-sized spherical-shaped palladium nanoparticles stick well to reduced graphene oxide sheets, which is consistent and correlated well with the XRD pattern. Moreover, a high-resolution morphological image of RGO-PN100 was obtained by TEM analysis, which shows the anchoring of palladium nanoparticles (PN) on RGO nanosheets. Green synthesized RGO-PN100 nanoparticles from Coleus amboinicus show better reduction kinetics for 4-nitrophenol at 40 min, suggesting that RGO-PN prepared from Coleus amboinicus serve as an excellent catalytic reducing agent. Furthermore, they show remarkable antibacterial activity against Escherichia coli (ATCC 25922). Thus, green synthesized RGO-supported palladium nanoparticles demonstrated that enhanced catalytic activity and antibacterial activity both play an important role in the environmental and medical disciplines.

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Facile one pot microbe-mediated in situ synthesis and antibacterial activity of reduced graphene oxide-silver nanocomposite

Nanotechnology ◽

10.1088/1361-6528/ac4521 ◽

2021 ◽

Author(s):

Ashwini Patil

Keyword(s):

Antibacterial Activity ◽

Graphene Oxide ◽

Zeta Potential ◽

Reduced Graphene Oxide ◽

In Vitro Dissolution ◽

Size Analysis ◽

X Ray ◽

Reduced Graphene

Abstract The present research deals with the development of a novel bioinspired in situ fabrication of reduced graphene oxide (rGO)-silver nanoparticle (AgNPs) nanocomposite (rGO@AgNCs) using microbes namely Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA). The fabricated rGO@AgNCs were characterized using Ultraviolet-visible (UV) spectroscopy, Fourier-transform infrared spectroscopy (FTIR), particle size analysis, polydispersity index (PDI), zeta potential analysis, energy dispersive X-ray analysis (EDAX), Raman spectroscopy, powder X-ray diffraction (PXRD), high-resolution transmission electron microscopy (HR-TEM) analysis, etc. Furthermore, the rGO@AgNCs-PA and rGO@AgNCs-SA interaction with serum protein, pH stability study, and in vitro dissolution of AgNPs were also performed. The research findings of the proposed study demonstrated the simultaneous reduction of graphene oxide (GO) and AgNPs and the formation of rGO@AgNCs in the presence of microbes. The in vitro dissolution studies of rGO@AgNCs composites showed better AgNPs dissolution with controlled release and offered remarkable matrix integrity throughout the dissolution period. The size and stability of rGO@AgNCs-PA and rGO@AgNCs-SA had no significant changes at physiological pH 7.4. A minimal decrease in the zeta potential of rGO@AgNCs was observed, which may be due to the weak interaction of nanocomposites and albumin. The antibacterial application of the synthesized nanocomposite was evaluated against a pathogenic mastitis-forming bacterium. The obtained results suggested an admirable antibacterial activity of synthesized nanocomposites against the tested microbes. This knowledge will assist the scientific fraternity in designing novel antibacterial agents with enhanced antibacterial activity against various veterinary pathogens in near future.

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Cd-Gd-doped nickel spinel ferrite nanoparticles and their nanocomposites with reduced graphene oxide for catalysis and antibacterial activity studies

Ceramics International ◽

10.1016/j.ceramint.2020.12.085 ◽

2020 ◽

Author(s):

Abdur Rahman ◽

Sonia Zulfiqar ◽

Ain Ul Haq ◽

Ibrahim A. Alsafari ◽

Umair Yaqub Qazi ◽

...

Keyword(s):

Antibacterial Activity ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Spinel Ferrite ◽

Ferrite Nanoparticles ◽

Reduced Graphene

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Antibacterial activity of Ag nanoparticles/phosphomolybdate/reduced graphene oxide nanocomposite: Kinetics and mechanism insights

Materials Science and Engineering B ◽

10.1016/j.mseb.2020.114709 ◽

2020 ◽

Vol 262 ◽

pp. 114709 ◽

Cited By ~ 1

Author(s):

Marjan Moghayedi ◽

Elaheh K. Goharshadi ◽

Kiarash Ghazvini ◽

Hossein Ahmadzadeh ◽

Majid Namayandeh Jorabchi

Keyword(s):

Antibacterial Activity ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Ag Nanoparticles ◽

Kinetics And Mechanism ◽

Reduced Graphene

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Reduced Graphene Oxide/Zinc Oxide Nanocomposite: From Synthesis to its Application for Wastewater Purification and Antibacterial Activity

Journal of Inorganic and Organometallic Polymers and Materials ◽

10.1007/s10904-020-01529-2 ◽

2020 ◽

Vol 30 (10) ◽

pp. 3907-3919 ◽

Cited By ~ 5

Author(s):

Taiba Naseem ◽

Zain-ul-Abdin ◽

Muhammad Waseem ◽

Muhammad Hafeez ◽

Salah Ud Din ◽

...

Keyword(s):

Zinc Oxide ◽

Antibacterial Activity ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Wastewater Purification ◽

Reduced Graphene

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Controlled release and long-term antibacterial activity of reduced graphene oxide/quaternary ammonium salt nanocomposites prepared by non-covalent modification

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2016.10.016 ◽

2017 ◽

Vol 149 ◽

pp. 322-329 ◽

Cited By ~ 30

Author(s):

Xiaoli Ye ◽

Jin Feng ◽

Jingxian Zhang ◽

Xiujiang Yang ◽

Xiaoyan Liao ◽

...

Keyword(s):

Antibacterial Activity ◽

Graphene Oxide ◽

Controlled Release ◽

Reduced Graphene Oxide ◽

Ammonium Salt ◽

Quaternary Ammonium ◽

Quaternary Ammonium Salt ◽

Covalent Modification ◽

Reduced Graphene

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Degradation photocatalysis of tetrodotoxin as a poison by gold doped PdO nanoparticles supported on reduced graphene oxide nanocomposites and evaluation of its antibacterial activity

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2016.12.027 ◽

2017 ◽

Vol 167 ◽

pp. 58-63 ◽

Cited By ~ 20

Author(s):

Ali Fakhri ◽

Mahsa Naji

Keyword(s):

Antibacterial Activity ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Reduced Graphene

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The effects of concentration, contact time, and pH value on antibacterial activity of silver nanoparticles decorated reduced graphene oxide

Materials Technology ◽

10.1080/10667857.2019.1630898 ◽

2019 ◽

Vol 34 (13) ◽

pp. 792-799 ◽

Cited By ~ 1

Author(s):

Nguyen Minh Dat ◽

Vu Ngoc Phuong Linh ◽

Nguyen Thi Lien Phuong ◽

Le Ngoc Quan ◽

Nguyen Thanh Huong ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Contact Time ◽

Ph Value ◽

Reduced Graphene

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Microwave Irradiation Synthesis and Characterization of Reduced-(Graphene Oxide-(Polystyrene-Polymethyl Methacrylate))/Silver Nanoparticle Nanocomposites and Their Anti-Microbial Activity

Polymers ◽

10.3390/polym12051155 ◽

2020 ◽

Vol 12 (5) ◽

pp. 1155 ◽

Cited By ~ 1

Author(s):

Mohammad A. Aldosari ◽

Khaled Bin Bandar Alsaud ◽

Ali Othman ◽

Mohammed Al-Hindawi ◽

Nadimul Haque Faisal ◽

...

Keyword(s):

Antibacterial Activity ◽

Graphene Oxide ◽

Microwave Irradiation ◽

Reduced Graphene Oxide ◽

Inhibition Zone ◽

Bulk Polymerization ◽

Reduced Graphene ◽

Methacrylate Copolymer ◽

K 12

Herein, we report a facile process for the preparation of styrene and methyl-methacrylate copolymer nanocomposites containing reduced graphene oxide and silver nanoparticles ((R-(GO-(PS-PMMA))/AgNPs)) by using (i) microwave irradiation (MWI) to obtain R-(GO-(PS-PMMA))/AgNPs and (ii) the in situ bulk polymerization technique to produce RGO/AgNPs-(PS-PMMA). Various characterization techniques, including FT-IR, XPS, Raman spectroscopy, XRD, SEM, HR-TEM, DSC, and TGA analysis, were used to characterize the prepared nanocomposites. The Berkovich nanoindentation method was employed to determine the hardness and elastic modulus of the nanocomposites. The results showed that the MWI-produced nanocomposites were found to have enhanced morphological, structural, and thermal properties compared with those of the nanocomposites prepared by the in situ method. In addition, the antibacterial activity of the prepared nanocomposites against the E. coli HB 101 K-12 was investigated, whereby an inhibition zone of 3 mm (RGO/AgNPs-(PS-PMMA) and 27 mm (R-(GO-(PS-PMMA))/AgNPs) was achieved. This indicates that the MWI-prepared nanocomposite has stronger antibacterial activity than the in situ-prepared nanocomposite.

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