Facile one pot microbe-mediated in situ synthesis and antibacterial activity of reduced graphene oxide-silver nanocomposite

2021 ◽  
Author(s):  
Ashwini Patil
Keyword(s):  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Zeta Potential ◽  
Reduced Graphene Oxide ◽  
In Vitro Dissolution ◽  
Size Analysis ◽  
X Ray ◽  
Reduced Graphene

Abstract The present research deals with the development of a novel bioinspired in situ fabrication of reduced graphene oxide (rGO)-silver nanoparticle (AgNPs) nanocomposite (rGO@AgNCs) using microbes namely Pseudomonas aeruginosa (PA) and Staphylococcus aureus (SA). The fabricated rGO@AgNCs were characterized using Ultraviolet-visible (UV) spectroscopy, Fourier-transform infrared spectroscopy (FTIR), particle size analysis, polydispersity index (PDI), zeta potential analysis, energy dispersive X-ray analysis (EDAX), Raman spectroscopy, powder X-ray diffraction (PXRD), high-resolution transmission electron microscopy (HR-TEM) analysis, etc. Furthermore, the rGO@AgNCs-PA and rGO@AgNCs-SA interaction with serum protein, pH stability study, and in vitro dissolution of AgNPs were also performed. The research findings of the proposed study demonstrated the simultaneous reduction of graphene oxide (GO) and AgNPs and the formation of rGO@AgNCs in the presence of microbes. The in vitro dissolution studies of rGO@AgNCs composites showed better AgNPs dissolution with controlled release and offered remarkable matrix integrity throughout the dissolution period. The size and stability of rGO@AgNCs-PA and rGO@AgNCs-SA had no significant changes at physiological pH 7.4. A minimal decrease in the zeta potential of rGO@AgNCs was observed, which may be due to the weak interaction of nanocomposites and albumin. The antibacterial application of the synthesized nanocomposite was evaluated against a pathogenic mastitis-forming bacterium. The obtained results suggested an admirable antibacterial activity of synthesized nanocomposites against the tested microbes. This knowledge will assist the scientific fraternity in designing novel antibacterial agents with enhanced antibacterial activity against various veterinary pathogens in near future.

scholarly journals Microwave Irradiation Synthesis and Characterization of Reduced-(Graphene Oxide-(Polystyrene-Polymethyl Methacrylate))/Silver Nanoparticle Nanocomposites and Their Anti-Microbial Activity

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1155 ◽  
Author(s):  
Mohammad A. Aldosari ◽  
Khaled Bin Bandar Alsaud ◽  
Ali Othman ◽  
Mohammed Al-Hindawi ◽  
Nadimul Haque Faisal ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Microwave Irradiation ◽  
Reduced Graphene Oxide ◽  
Inhibition Zone ◽  
Bulk Polymerization ◽  
Reduced Graphene ◽  
Methacrylate Copolymer ◽  
K 12

Herein, we report a facile process for the preparation of styrene and methyl-methacrylate copolymer nanocomposites containing reduced graphene oxide and silver nanoparticles ((R-(GO-(PS-PMMA))/AgNPs)) by using (i) microwave irradiation (MWI) to obtain R-(GO-(PS-PMMA))/AgNPs and (ii) the in situ bulk polymerization technique to produce RGO/AgNPs-(PS-PMMA). Various characterization techniques, including FT-IR, XPS, Raman spectroscopy, XRD, SEM, HR-TEM, DSC, and TGA analysis, were used to characterize the prepared nanocomposites. The Berkovich nanoindentation method was employed to determine the hardness and elastic modulus of the nanocomposites. The results showed that the MWI-produced nanocomposites were found to have enhanced morphological, structural, and thermal properties compared with those of the nanocomposites prepared by the in situ method. In addition, the antibacterial activity of the prepared nanocomposites against the E. coli HB 101 K-12 was investigated, whereby an inhibition zone of 3 mm (RGO/AgNPs-(PS-PMMA) and 27 mm (R-(GO-(PS-PMMA))/AgNPs) was achieved. This indicates that the MWI-prepared nanocomposite has stronger antibacterial activity than the in situ-prepared nanocomposite.

scholarly journals Antibacterial Performance of a Mussel-Inspired Polydopamine-Treated Ag/Graphene Nanocomposite Material

Materials ◽  
2019 ◽  
Vol 12 (20) ◽  
pp. 3360
Author(s):  
Jianming Liao ◽  
Shuaiming He ◽  
Shasha Guo ◽  
Pengcheng Luan ◽  
Lihuan Mo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Antibacterial Properties ◽  
Great Promise ◽  
X Ray ◽  
Antibacterial Performance ◽  
Reduced Graphene ◽  
Wide Range ◽  
Graphene Nanocomposite

Graphene-based nanocomposites have attracted tremendous attention in recent years. In this study, a facile yet effective approach was developed to synthesize reduced graphene oxide and an Ag–graphene nanocomposite. The basic strategy involved in the preparation of reduced graphene oxide includes reducing graphene oxide with dopamine, followed by in situ syntheses of the Ag-PDA-reducing graphene oxide (RGO) nanocomposite through adding AgNO3 solution and a small amount of dopamine. The nanocomposite was characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray diffraction (XRD), FTIR spectra, Raman spectra, ultraviolet-visible (UV-vis), and X-ray photoelectron spectroscopy (XPS), results indicated that a uniform PDA film is formed on the surface of the GO and GO is successfully reduced. Besides, the in situ synthesized Ag nanoparticles (AgNPs) were evenly distributed on the RGO surface. To investigate antibacterial properties Ag-PDA-RGO, different dosages were selected for evaluating the antibacterial activity against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli. The Ag-PDA-RGO nanocomposites displayed excellent antibacterial property. The antibacterial ratio reached 99.9% against S. aureus and 90.9% against E. coli when the dosage of 100 mg/L Ag-PDA-RGO nanocomposites was 100 μL. The novel Ag-PDA-RGO nanocomposite prepared by a facile yet effective, environmentally friendly, and low-cost method holds great promise in a wide range of modern biomedical applications.

Electrochemical and in situ X-ray spectroscopic studies of MnO2/reduced graphene oxide nanocomposites as a supercapacitor

2016 ◽  
Vol 18 (28) ◽  
pp. 18705-18718 ◽  
Author(s):  
Han-Wei Chang ◽  
Ying-Rui Lu ◽  
Jeng-Lung Chen ◽  
Chi-Liang Chen ◽  
Jyh-Fu Lee ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Spectroscopic Studies ◽  
X Ray ◽  
Reduced Graphene ◽  
Charge Discharge ◽  
Carbon Based

An in situ XAS investigation reveals charge–discharge mechanisms of MnO2/carbon-based materials.

scholarly journals Co2TiO4/Reduced Graphene Oxide Nanohybrids for Electrochemical Sensing Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1611
Author(s):  
Constanza J. Venegas ◽  
Fabiana A. Gutierrez ◽  
Marcos Eguílaz ◽  
José F. Marco ◽  
Nik Reeves-McLaren ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Hydrogen Peroxide ◽  
Scanning Electron Microscopy ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Hybrid Materials ◽  
X Ray ◽  
Reduced Graphene ◽  
Scanning Electron

For the first time, the synthesis, characterization, and analytical application for hydrogen peroxide quantification of the hybrid materials of Co2TiO4 (CTO) and reduced graphene oxide (RGO) is reported, using in situ (CTO/RGO) and ex situ (CTO+RGO) preparations. This synthesis for obtaining nanostructured CTO is based on a one-step hydrothermal synthesis, with new precursors and low temperatures. The morphology, structure, and composition of the synthesized materials were examined using scanning electron microscopy, X-ray diffraction (XRD), neutron powder diffraction (NPD), and X-ray photoelectron spectroscopy (XPS). Rietveld refinements using neutron diffraction data were conducted to determine the cation distributions in CTO. Hybrid materials were also characterized by Brunauer–Emmett–Teller adsorption isotherms, Scanning Electron microscopy, and scanning electrochemical microscopy. From an analytical point of view, we evaluated the electrochemical reduction of hydrogen peroxide on glassy carbon electrodes modified with hybrid materials. The analytical detection of hydrogen peroxide using CTO/RGO showed 11 and 5 times greater sensitivity in the detection of hydrogen peroxide compared with that of pristine CTO and RGO, respectively, and a two-fold increase compared with that of the RGO+CTO modified electrode. These results demonstrate that there is a synergistic effect between CTO and RGO that is more significant when the hybrid is synthetized through in situ methodology.

scholarly journals In Vitro Hyperthermia Evaluation of Electrospun Polymer Composite Fibers Loaded with Reduced Graphene Oxide

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2663
Author(s):  
Ignacio A. Zárate ◽  
Héctor Aguilar-Bolados ◽  
Mehrdad Yazdani-Pedram ◽  
Guadalupe del C. Pizarro ◽  
Andrónico Neira-Carrillo
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Near Infrared ◽  
X Ray Diffraction ◽  
Arabic Gum ◽  
X Ray ◽  
Average Temperature ◽  
Reduced Graphene ◽  
Excellent Candidate

Electrospun meshes (EM) composed of natural and synthetic polymers with randomly or aligned fibers orientations containing 0.5% or 1% of thermally reduced graphene oxide (TrGO) were prepared by electrospinning (ES), and their hyperthermia properties were evaluated. EM loaded with and without TrGO were irradiated using near infrared radiation (NIR) at 808 nm by varying the distance and electric potential recorded at 30 s. Morphological, spectroscopic, and thermal aspects of EM samples were analyzed by using SEM-EDS, Raman and X-ray photoelectron (XPS) spectroscopies, X-ray diffraction (XRD), and NIR radiation response. We found that the composite EM made of polyvinyl alcohol (PVA), natural rubber (NR), and arabic gum (AG) containing TrGO showed improved hyperthermia properties compared to EM without TrGO, reaching an average temperature range of 42–52 °C. We also found that the distribution of TrGO in the EM depends on the orientation of the fibers. These results allow infering that EM loaded with TrGO as a NIR-active thermal inducer could be an excellent candidate for hyperthermia applications in photothermal therapy.

scholarly journals Green syntheses of silver nanoparticle decorated reduced graphene oxide using l-methionine as a reducing and stabilizing agent for enhanced catalytic hydrogenation of 4-nitrophenol and antibacterial activity

RSC Advances ◽  
2019 ◽  
Vol 9 (67) ◽  
pp. 39264-39271 ◽  
Author(s):  
Neway Belachew ◽  
Desta Shumuye Meshesha ◽  
Keloth Basavaiah
Keyword(s):  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Silver Nanoparticle ◽  
Self Assembly ◽  
Stabilizing Agent ◽  
Assembly Method ◽  
Reduced Graphene ◽  
Synthesis Approach

Herein, we have reported a facile and green synthesis approach of Ag NP decorated reduced graphene oxide (RGO) through an in situ self-assembly method in the presence of l-methionine (l-Met) as reducing and stabilizing agent.

scholarly journals Biomimetic reduced graphene oxide coated collagen scaffold for in situ bone regeneration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sajad Bahrami ◽  
Nafiseh Baheiraei ◽  
Mostafa Shahrezaee
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Cranial Bone ◽  
Drying Methods ◽  
Porous Scaffolds ◽  
Alizarin Red ◽  
Reduced Graphene ◽  
Coated Collagen

AbstractA variety of bone-related diseases and injures and limitations of traditional regeneration methods require new tissue substitutes. Tissue engineering and regeneration combined with nanomedicine can provide different natural or synthetic and combined scaffolds with bone mimicking properties for implantation in the injured area. In this study, we synthesized collagen (Col) and reduced graphene oxide coated collagen (Col-rGO) scaffolds, and we evaluated their in vitro and in vivo effects on bone tissue repair. Col and Col-rGO scaffolds were synthesized by chemical crosslinking and freeze-drying methods. The surface topography, and the mechanical and chemical properties of scaffolds were characterized, showing three-dimensional (3D) porous scaffolds and successful coating of rGO on Col. The rGO coating enhanced the mechanical strength of Col-rGO scaffolds to a greater extent than Col scaffolds by 2.8 times. Furthermore, Col-rGO scaffolds confirmed that graphene addition induced no cytotoxic effects and enhanced the viability and proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) with 3D adherence and expansion. Finally, scaffold implantation into rabbit cranial bone defects for 12 weeks showed increased bone formation, confirmed by Hematoxylin–Eosin (H&E) and alizarin red staining. Overall, the study showed that rGO coating improves Col scaffold properties and could be a promising implant for bone injuries.

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