scholarly journals Microfluidics for Peptidomics, Proteomics, and Cell Analysis

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1118
Author(s):  
Rui Vitorino ◽  
Sofia Guedes ◽  
João Pinto da Costa ◽  
Václav Kašička
Keyword(s):  
Single Cell Analysis ◽  
Disease Diagnosis ◽  
Detection Methods ◽  
Cell Analysis ◽  
Microfluidic Platforms ◽  
Recent Developments ◽  
Peptidomic Analysis ◽  
New Applications ◽  
Fluid Manipulation

Microfluidics is the advanced microtechnology of fluid manipulation in channels with at least one dimension in the range of 1–100 microns. Microfluidic technology offers a growing number of tools for manipulating small volumes of fluid to control chemical, biological, and physical processes relevant to separation, analysis, and detection. Currently, microfluidic devices play an important role in many biological, chemical, physical, biotechnological and engineering applications. There are numerous ways to fabricate the necessary microchannels and integrate them into microfluidic platforms. In peptidomics and proteomics, microfluidics is often used in combination with mass spectrometric (MS) analysis. This review provides an overview of using microfluidic systems for peptidomics, proteomics and cell analysis. The application of microfluidics in combination with MS detection and other novel techniques to answer clinical questions is also discussed in the context of disease diagnosis and therapy. Recent developments and applications of capillary and microchip (electro)separation methods in proteomic and peptidomic analysis are summarized. The state of the art of microchip platforms for cell sorting and single-cell analysis is also discussed. Advances in detection methods are reported, and new applications in proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices and determination of their physicochemical parameters are highlighted.

scholarly journals Advances in Single-Cell Printing

Micromachines ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 80
Author(s):  
Xiaohu Zhou ◽  
Han Wu ◽  
Haotian Wen ◽  
Bo Zheng
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Three Dimensional ◽  
Cell Isolation ◽  
Special Focus ◽  
Cell Analysis ◽  
Cell Array ◽  
Cell Printing ◽  
Recent Developments ◽  
Single Cell Isolation

Single-cell analysis is becoming an indispensable tool in modern biological and medical research. Single-cell isolation is the key step for single-cell analysis. Single-cell printing shows several distinct advantages among the single-cell isolation techniques, such as precise deposition, high encapsulation efficiency, and easy recovery. Therefore, recent developments in single-cell printing have attracted extensive attention. We review herein the recently developed bioprinting strategies with single-cell resolution, with a special focus on inkjet-like single-cell printing. First, we discuss the common cell printing strategies and introduce several typical and advanced printing strategies. Then, we introduce several typical applications based on single-cell printing, from single-cell array screening and mass spectrometry-based single-cell analysis to three-dimensional tissue formation. In the last part, we discuss the pros and cons of the single-cell strategies and provide a brief outlook for single-cell printing.

scholarly journals Recent advances in the use of microfluidic technologies for single cell analysis

The Analyst ◽  
2018 ◽  
Vol 143 (1) ◽  
pp. 60-80 ◽  
Author(s):  
Travis W. Murphy ◽  
Qiang Zhang ◽  
Lynette B. Naler ◽  
Sai Ma ◽  
Chang Lu
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Microfluidic Platforms ◽  
Recent Advances

We present a review on recent advances in single cell analysis based on microfluidic platforms.

Recent developments in single-cell analysis

2004 ◽  
Vol 510 (2) ◽  
pp. 127-138 ◽  
Author(s):  
Xin Lu ◽  
Wei-Hua Huang ◽  
Zong-Li Wang ◽  
Jie-Ke Cheng
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Recent Developments

scholarly journals Preparation of Tissues and Heterogeneous Cellular Samples for Single-Cell Analysis

2021 ◽  
Author(s):  
E. Celeste Welch ◽  
Anubhav Tripathi
Keyword(s):  
Sample Preparation ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Cell Analysis ◽  
Processing Times ◽  
Tissue Dissociation ◽  
Emerging Trends ◽  
Recent Developments ◽  
Preparation Techniques

While sample preparation techniques for the chemical and biochemical analysis of tissues are fairly well advanced, the preparation of complex, heterogenous samples for single-cell analysis can be difficult and challenging. Nevertheless, there is growing interest in preparing complex cellular samples, particularly tissues, for analysis via single-cell resolution techniques such as single-cell sequencing or flow cytometry. Recent microfluidic tissue dissociation approaches have helped to expedite the preparation of single cells from tissues through the use of optimized, controlled mechanical forces. Cell sorting and selective cellular recovery from heterogenous samples have also gained traction in biosensors, microfluidic systems, and other diagnostic devices. Together, these recent developments in tissue disaggregation and targeted cellular retrieval have contributed to the development of increasingly streamlined sample preparation workflows for single-cell analysis technologies, which minimize equipment requirements, enable lower processing times and costs, and pave the way for high-throughput, automated technologies. In this chapter, we survey recent developments and emerging trends in this field.

scholarly journals An Automated Microwell Platform for Large-Scale Single Cell RNA-Seq

2016 ◽  
Author(s):  
Jinzhou Yuan ◽  
Peter A. Sims
Keyword(s):  
Single Cell ◽  
Expression Profiling ◽  
Large Scale ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Cell Capture ◽  
Rna Seq ◽  
Fluorescent Cell ◽  
Recent Developments ◽  
Improved Performance

Recent developments have enabled rapid, inexpensive RNA sequencing of thousands of individual cells from a single specimen, raising the possibility of unbiased and comprehensive expression profiling from complex tissues. Microwell arrays are a particularly attractive microfluidic platform for single cell analysis due to their scalability, cell capture efficiency, and compatibility with imaging. We report an automated microwell array platform for single cell RNA-Seq with significantly improved performance over previous implementations. We demonstrate cell capture efficiencies of >50%, compatibility with commercially available barcoded mRNA capture beads, and parallel expression profiling from thousands of individual cells. We evaluate the level of cross-contamination in our platform by both tracking fluorescent cell lysate in sealed microwells and with a human-mouse mixed species RNA-Seq experiment. Finally, we apply our system to comprehensively assess heterogeneity in gene expression of patient-derived glioma neurospheres and uncover subpopulations similar to those observed in human glioma tissue.

Isolation of trophoblast cells from transcervical specimens for prenatal determination of fetal sex by single-cell analysis

2012 ◽  
Vol 98 (3) ◽  
pp. S133
Author(s):  
J.M. Bolnick ◽  
B.A. Kilburn ◽  
S. Bajpayee ◽  
N. Reddy ◽  
M.P. Diamond ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Trophoblast Cells ◽  
Fetal Sex

Frontier microfluidic techniques for short and long-term single cell analysis

Lab on a Chip ◽  
2014 ◽  
Vol 14 (13) ◽  
pp. 2161-2167 ◽  
Author(s):  
Jonathan Avesar ◽  
Tom Ben Arye ◽  
Shulamit Levenberg
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Microfluidic Platforms ◽  
Short And Long Term ◽  
Technological Improvements

This review details the frontier microfluidic platforms for single cell analysis, highlighting technological improvements and cell analysis capabilities.

Microchemical analytical techniques of potential clinical interest.

1977 ◽  
Vol 23 (8) ◽  
pp. 1465-1471 ◽  
Author(s):  
D Glick
Keyword(s):  
Single Cell Analysis ◽  
Clinical Laboratory ◽  
Analytical Techniques ◽  
General System ◽  
Chemical Information ◽  
Cell Analysis ◽  
Laboratory Practice ◽  
Clinical Interest ◽  
Reaction In Solution

Abstract Microchemical techniques with the capability of single-cell analysis will become increasingly important in clinical laboratory practice. One of the current moves underway in this direction is determination of enzyme activities of cells by microscale spectrophotometry and spectrophotofluorometry for prenatal diagnosis of inherited enzyme deficiencies. Three less-common microanalytical techniques are considered here that are of potential interest in laboratory medicine. The first is dilatometry, which provides a means for enzyme or substrate assay involving measurement of the change in volume or density accompanying chemical reaction in solution. In this connection, the measurement of specific gravity itself to obtain certain chemical information is also of interest. The second of these techniques is the use of spectrophotometry to measure oxygen uptake for functional assays of cells. The third is the use of luminometry in a general system of analysis for determination of many important biochemical substances and activities. Each of these techniques can be used for microscale analysis without sacrifice of precision or accuracy; each is relatively simple, instrumentally ant technically, and could be automated.

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