scholarly journals Advances in Single-Cell Printing

Micromachines ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 80
Author(s):  
Xiaohu Zhou ◽  
Han Wu ◽  
Haotian Wen ◽  
Bo Zheng
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Three Dimensional ◽  
Cell Isolation ◽  
Special Focus ◽  
Cell Analysis ◽  
Cell Array ◽  
Cell Printing ◽  
Recent Developments ◽  
Single Cell Isolation

Single-cell analysis is becoming an indispensable tool in modern biological and medical research. Single-cell isolation is the key step for single-cell analysis. Single-cell printing shows several distinct advantages among the single-cell isolation techniques, such as precise deposition, high encapsulation efficiency, and easy recovery. Therefore, recent developments in single-cell printing have attracted extensive attention. We review herein the recently developed bioprinting strategies with single-cell resolution, with a special focus on inkjet-like single-cell printing. First, we discuss the common cell printing strategies and introduce several typical and advanced printing strategies. Then, we introduce several typical applications based on single-cell printing, from single-cell array screening and mass spectrometry-based single-cell analysis to three-dimensional tissue formation. In the last part, we discuss the pros and cons of the single-cell strategies and provide a brief outlook for single-cell printing.

Recent developments in single-cell analysis

2004 ◽  
Vol 510 (2) ◽  
pp. 127-138 ◽  
Author(s):  
Xin Lu ◽  
Wei-Hua Huang ◽  
Zong-Li Wang ◽  
Jie-Ke Cheng
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Recent Developments

Densely-Packed Microbowl Array with Balanced Dielectrophoretic Forces for Single-Cell Microarray

2009 ◽  
Vol 1222 ◽  
Author(s):  
Maesoon Im ◽  
Dong-Haan Kim ◽  
Joo-Hyung Lee ◽  
Jun-Bo Yoon ◽  
Yang-Kyu Choi
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Three Dimensional ◽  
Average Diameter ◽  
Experimental Result ◽  
Cell Analysis ◽  
Large Area ◽  
Latex Beads ◽  
Nickel Electroplating ◽  
Cell Microarray

AbstractIn this paper, we demonstrate a perfectly-ordered microbowl array with balanced dielectrophoresis (DEP) for a high-throughput single-cell analysis. In order to fabricate well-ordered microbowl array in a large area, we utilized three-dimensional diffuser lithography for photoresist mold and nickel electroplating technique for final microbowl structures on a silicon substrate. Single microbowl has six sharp apexes surrounding the microbowl perimeter. Each microbowl has a diameter of 10 μm, and a height of 9 μm, which can be controllable by patterns on mask and lithography conditions. To investigate feasibility for application to the microbowl array as a single-cell microarray, we used latex beads of 6.4 μm in an average diameter to be captured by dielectrophoretic force. The nickel microbowl array densely packed with a hexagonal geometry played as a bottom electrode, and an ITO-coated glass covered the nickel microbowl array as a top electrode while keeping a uniform gap between two electrodes. After injecting solution containing latex beads through the gap, we applied an AC signal (2 VPP, 1 MHz) between two electrodes to induce high electric field near the sharp apexes of the single microbowl. A negative DEP trap is formed at the center of the single microbowl with balanced DEP force from the six apexes. The experimental result shows that injected latex beads had been successfully and uniformly aligned and trapped at the microbowl array sustained by negative DEP.

scholarly journals Preparation of Tissues and Heterogeneous Cellular Samples for Single-Cell Analysis

2021 ◽  
Author(s):  
E. Celeste Welch ◽  
Anubhav Tripathi
Keyword(s):  
Sample Preparation ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Cell Analysis ◽  
Processing Times ◽  
Tissue Dissociation ◽  
Emerging Trends ◽  
Recent Developments ◽  
Preparation Techniques

While sample preparation techniques for the chemical and biochemical analysis of tissues are fairly well advanced, the preparation of complex, heterogenous samples for single-cell analysis can be difficult and challenging. Nevertheless, there is growing interest in preparing complex cellular samples, particularly tissues, for analysis via single-cell resolution techniques such as single-cell sequencing or flow cytometry. Recent microfluidic tissue dissociation approaches have helped to expedite the preparation of single cells from tissues through the use of optimized, controlled mechanical forces. Cell sorting and selective cellular recovery from heterogenous samples have also gained traction in biosensors, microfluidic systems, and other diagnostic devices. Together, these recent developments in tissue disaggregation and targeted cellular retrieval have contributed to the development of increasingly streamlined sample preparation workflows for single-cell analysis technologies, which minimize equipment requirements, enable lower processing times and costs, and pave the way for high-throughput, automated technologies. In this chapter, we survey recent developments and emerging trends in this field.

Microscale Oil-Covered Cell Array (MOCCA): A Droplet Array for High-Content Single-Cell Analysis and Imaging

2009 ◽  
Author(s):  
Liang-I Lin ◽  
Shih-Hui Chao ◽  
Deirdre R. Meldrum
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Low Cost ◽  
Cell Number ◽  
Cell Analysis ◽  
Cell Array ◽  
Flat Substrate ◽  
Fabrication Procedure ◽  
Droplet Array ◽  
The Cost

A simple, low-cost technique for high throughput single-cell analysis, Microscale Oil-Covered Cell Array (MOCCA), is presented in this paper. Corresponding to recent research on single cell analysis, simple devices for isolated cell chambers are urgently needed and long sought-after. Instead of using microfabricated solid structures to capture cells, MOCCA isolates cells in discrete aqueous droplets that are separated by oil on the patterned hydrophilic areas on a relatively more hydrophobic flat substrate. In our pioneer study, we created an array of 700-picoliter droplets. The randomly seeded E. coli cell number in each discrete droplet approaches single-cell levels. The total time needed for MOCCA fabrication was no more than 10 minutes. Compared to traditional micro-fabrication techniques, MOCCA dramatically lowers the cost and enhances the efficiency for the fabrication procedure, while producing a microscale array as in those made using traditional methods.

scholarly journals An Automated Microwell Platform for Large-Scale Single Cell RNA-Seq

2016 ◽  
Author(s):  
Jinzhou Yuan ◽  
Peter A. Sims
Keyword(s):  
Single Cell ◽  
Expression Profiling ◽  
Large Scale ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Cell Capture ◽  
Rna Seq ◽  
Fluorescent Cell ◽  
Recent Developments ◽  
Improved Performance

Recent developments have enabled rapid, inexpensive RNA sequencing of thousands of individual cells from a single specimen, raising the possibility of unbiased and comprehensive expression profiling from complex tissues. Microwell arrays are a particularly attractive microfluidic platform for single cell analysis due to their scalability, cell capture efficiency, and compatibility with imaging. We report an automated microwell array platform for single cell RNA-Seq with significantly improved performance over previous implementations. We demonstrate cell capture efficiencies of >50%, compatibility with commercially available barcoded mRNA capture beads, and parallel expression profiling from thousands of individual cells. We evaluate the level of cross-contamination in our platform by both tracking fluorescent cell lysate in sealed microwells and with a human-mouse mixed species RNA-Seq experiment. Finally, we apply our system to comprehensively assess heterogeneity in gene expression of patient-derived glioma neurospheres and uncover subpopulations similar to those observed in human glioma tissue.

Universally applicable three-dimensional hydrodynamic focusing in a single-layer channel for single cell analysis

2018 ◽  
Vol 10 (28) ◽  
pp. 3489-3497 ◽  
Author(s):  
Yingying Zhao ◽  
Qin Li ◽  
Xiaoming Hu
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Three Dimensional ◽  
Single Layer ◽  
Optical Systems ◽  
Cell Analysis ◽  
Hydrodynamic Focusing ◽  
Integrated Optical ◽  
Microfluidic Structure

A microfluidic cytometer which integrated 3D hydrodynamic focusing and integrated optical systems on a single-layer microfluidic structure was demonstrated.

Three Dimensional Manipulation of Cells Using Holographic Optical Tweezers

2012 ◽  
Vol 241-244 ◽  
pp. 513-516
Author(s):  
Tao Tao ◽  
Jing Li ◽  
Yang Lin
Keyword(s):  
Single Cell ◽  
Optical Tweezers ◽  
Single Cell Analysis ◽  
Three Dimensional ◽  
Optic Axis ◽  
Three Dimensions ◽  
Yeast Cells ◽  
Optical Traps ◽  
Cell Analysis ◽  
Holographic Optical Tweezers

A holographic optical tweezers platform was built and a multi-plane adaptive-additive algorithm was used to generating holograms for the reconstruction of optical traps in three-dimensional (3D) spaces. Experiments of manipulating cells were conducted on such platform and complex 3D structures were built with yeast cells. The results demonstrate that holographic optical tweezers can manipulate groups of cells in three dimensions and effectively trap and separate cells in the direction of the optic axis without harming the cells. Based on those versatile functions, it is proved that holographic optical tweezers is a powerful tool for single cell analysis.

scholarly journals Single-cell analysis of intestinal immune cells during helminth infection

2019 ◽  
Author(s):  
Laura Ferrer-Font ◽  
Palak Mehta ◽  
Phoebe Harmos ◽  
Alfonso Schmidt ◽  
Kylie M Price ◽  
...  
Keyword(s):  
Immune Responses ◽  
Single Cell ◽  
Immune Cells ◽  
Helminth Infection ◽  
Immune Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Mucus Production ◽  
Single Cell Isolation

AbstractSingle cell isolation from helminth infected intestines has been notoriously difficult, due to the strong anti-parasite type 2 immune responses that drive mucus production, tissue remodeling and immune cell infiltration. Through the systematic optimization of a standard intestinal digestion protocol, we were able to isolate millions of immune cells from heavily infected tissues. Using this protocol, we validated many hallmarks of anti-parasite immunity and analyzed immune cells from the lamina propria and granulomas during helminth development, as well as acute and chronic worm infection.

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