Single-Walled Carbon Nanotubes Inhibit TRPC4-Mediated Muscarinic Cation Current in Mouse Ileal Myocytes

Single-walled carbon nanotubes (SWCNTs) are characterized by a combination of rather unique physical and chemical properties, which makes them interesting biocompatible nanostructured materials for various applications, including in the biomedical field. SWCNTs are not inert carriers of drug molecules, as they may interact with various biological macromolecules, including ion channels. To investigate the mechanisms of the inhibitory effects of SWCNTs on the muscarinic receptor cation current (mICAT), induced by intracellular GTPγs (200 μM), in isolated mouse ileal myocytes, we have used the patch-clamp method in the whole-cell configuration. Here, we use molecular docking/molecular dynamics simulations and direct patch-clamp recordings of whole-cell currents to show that SWCNTs, purified and functionalized by carboxylation in water suspension containing single SWCNTs with a diameter of 0.5–1.5 nm, can inhibit mICAT, which is mainly carried by TRPC4 cation channels in ileal smooth muscle cells, and is the main regulator of cholinergic excitation–contraction coupling in the small intestinal tract. This inhibition was voltage-independent and associated with a shortening of the mean open time of the channel. These results suggest that SWCNTs cause a direct blockage of the TRPC4 channel and may represent a novel class of TRPC4 modulators.

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Low-Energy Irradiation Damage in Single-Walled Carbon Nanotubes

MRS Proceedings ◽

10.1557/proc-0994-f04-02 ◽

2007 ◽

Vol 994 ◽

Cited By ~ 4

Author(s):

Satoru Suzuki ◽

Yoshihiro Kobayashi

Keyword(s):

Carbon Nanotubes ◽

Chemical Properties ◽

Single Walled Carbon Nanotubes ◽

Low Energy ◽

Irradiation Damage ◽

Single Walled Carbon ◽

Photon Irradiation ◽

Energy Irradiation ◽

Physical And Chemical ◽

Walled Carbon Nanotubes

AbstractWe show that low-energy (20 eV–20 keV) electron or photon irradiation extinguishes the characteristic physical and chemical properties of single-walled carbon nanotubes, indicating that the irradiation damages the nanotubes. The irradiation-induced defects convert the electric properties of metallic SWNTs to semiconducting, and the nominal bandgap can be tuned simply by the irradiation dose. The defects also have the following interesting properties. The damage and recovery are reversible, indicating that the number of carbon atoms is preserved. The damage and recovery strongly depend on the diameter, suggesting that the damage is prominent in a rolled up graphene sheet, but not in a planar one. The activation energy of the defect healing is so small, depending on the diameter, that the defects can be healed even at room temperature or below.

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Single-walled carbon nanotubes: differential genotoxic potential associated with physico-chemical properties

Nanotoxicology ◽

10.3109/17435390.2011.647928 ◽

2012 ◽

Vol 7 (2) ◽

pp. 144-156 ◽

Cited By ~ 28

Author(s):

Bella B. Manshian ◽

Gareth JS. Jenkins ◽

Paul M. Williams ◽

Chris Wright ◽

Andrew R. Barron ◽

...

Keyword(s):

Carbon Nanotubes ◽

Chemical Properties ◽

Single Walled Carbon Nanotubes ◽

Single Walled Carbon ◽

Genotoxic Potential ◽

Physico Chemical ◽

Walled Carbon Nanotubes

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Structural Analysis of Encapsulated Single-Walled Carbon Nanotubes

SAMRIDDHI A Journal of Physical Sciences Engineering and Technology ◽

10.18090/samriddhi.v2i2.1605 ◽

2015 ◽

Vol 2 (2) ◽

Author(s):

D. B. Singh ◽

V. N. Shukla ◽

Vikas Kumar ◽

Pragya Gupta ◽

L. Ramma

Keyword(s):

Carbon Nanotubes ◽

Chemical Properties ◽

Single Walled Carbon Nanotubes ◽

Radial Breathing Mode ◽

Peak Position ◽

Light Illumination ◽

Single Walled Carbon ◽

Raman Modes ◽

Tip Enhanced Raman Spectroscopy ◽

Walled Carbon Nanotubes

Tip-enhanced Raman spectroscopy revealed the nanoscale chemical properties of organic molecules encapsulated in single walled carbon nanotubes (SWNTs). Our approach is based on an enhanced electric field near a laser-irradiated metal tip functioning as a Raman excitation source. The enhanced field can successfully act on encapsulated molecules through the walls of the SWNTs to extract molecular vibrational information -carotene, which exhibits several active Raman modes under visible light illumination, was used as the encapsulated molecule. Tip-enhanced Raman spectra measured at seven different positions on SWNT bundles showed that carotene molecules inside the tubes were not uniformly distributed. It is also found that the filling rate and peak position of the radial breathing mode of the SWNTs are linearly correlated.

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Applications of Filled Single-Walled Carbon Nanotubes: Progress, Challenges, and Perspectives

Nanomaterials ◽

10.3390/nano11112863 ◽

2021 ◽

Vol 11 (11) ◽

pp. 2863

Author(s):

Marianna V. Kharlamova ◽

Christian Kramberger

Keyword(s):

Carbon Nanotubes ◽

Electronic Properties ◽

Light Emission ◽

Chemical Properties ◽

Single Walled Carbon Nanotubes ◽

Fine Tuning ◽

Single Walled Carbon ◽

Physical And Chemical ◽

Walled Carbon Nanotubes ◽

And Chemical Properties

Single-walled carbon nanotubes (SWCNTs), which possess electrical and thermal conductivity, mechanical strength, and flexibility, and are ultra-light weight, are an outstanding material for applications in nanoelectronics, photovoltaics, thermoelectric power generation, light emission, electrochemical energy storage, catalysis, sensors, spintronics, magnetic recording, and biomedicine. Applications of SWCNTs require nanotube samples with precisely controlled and customized electronic properties. The filling of SWCNTs is a promising approach in the fine-tuning of their electronic properties because a large variety of substances with appropriate physical and chemical properties can be introduced inside SWCNTs. The encapsulation of electron donor or acceptor substances inside SWCNTs opens the way for the Fermi-level engineering of SWCNTs for specific applications. This paper reviews the recent progress in applications of filled SWCNTs and highlights challenges that exist in the field.

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Physico-Chemical Properties of Iodine-Adsorbed Single-Walled Carbon Nanotubes

Langmuir ◽

10.1021/la803395a ◽

2009 ◽

Vol 25 (3) ◽

pp. 1795-1799 ◽

Cited By ~ 11

Author(s):

Chiharu Hayakawa ◽

Koki Urita ◽

Tomonori Ohba ◽

Hirofumi Kanoh ◽

Katsumi Kaneko

Keyword(s):

Carbon Nanotubes ◽

Chemical Properties ◽

Single Walled Carbon Nanotubes ◽

Single Walled Carbon ◽

Physico Chemical ◽

Walled Carbon Nanotubes

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A comparison of catabolic pathways induced in primary macrophages by pristine single walled carbon nanotubes and pristine graphene

RSC Advances ◽

10.1039/c6ra02476a ◽

2016 ◽

Vol 6 (70) ◽

pp. 65299-65310 ◽

Cited By ~ 8

Author(s):

Jennifer McIntyre ◽

Navin K. Verma ◽

Ronan J. Smith ◽

Caroline Moore ◽

Hannah Nerl ◽

...

Keyword(s):

Risk Assessment ◽

Carbon Nanotubes ◽

Chemical Properties ◽

Single Walled Carbon Nanotubes ◽

Pristine Graphene ◽

Catabolic Pathways ◽

Single Walled Carbon ◽

Physico Chemical ◽

Walled Carbon Nanotubes

Understanding the correlation between the physico-chemical properties of carbonaceous nanomaterials and how these properties impact on cells and subcelluar mechanisms is critical to their risk assessment and safe translation into engineered devices.

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