scholarly journals Genome Sequence and Adaptation Analysis of the Human and Rice Pathogenic Strain Burkholderia glumae AU6208

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 87
Author(s):  
Zhouqi Cui ◽  
Sai Wang ◽  
Kaleem Ullah Kakar ◽  
Guanglin Xie ◽  
Bin Li ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Type Strain ◽  
Antibiotic Biosynthesis ◽  
Chronic Infections ◽  
Burkholderia Glumae ◽  
Niche Adaptation ◽  
History Of ◽  
Clinical Conditions ◽  
Important Disease ◽  
Panicle Blight

Burkholderia glumae causes rice (Oryza sativa) bacterial panicle blight, which is an increasingly economically important disease worldwide. As the first B. glumae strain isolated from patients with chronic infections, AU6208 has been reported as an opportunistic clinic pathogen. However, our understanding of the molecular mechanism underlying human pathogenesis by B. glumae remains rudimentary. In this study, we report the complete genome sequence of the human-isolated B. glumae strain AU6208 and compare this to the genome of the rice-pathogenic B. glumae type strain LMG 2196T. Analysis of the average nucleotide identity demonstrated 99.4% similarity between the human- and plant-pathogenic strains. However, the phenotypic results from this study suggest a history of niche adaptation and divergence. In particular, we found 44 genes were predicted to be horizontally transferred into AU6208, and most of these genes were upregulated in conditions that mimic clinical conditions. In these, the gene pair sbnAB encodes key enzymes in antibiotic biosynthesis. These results suggest that horizontal gene transfer in AU6208 may be responsible for selective advantages in its pathogenicity in humans. Our analysis of the AU6208 genome and comparison with that of LMG 2196T reveal the evolutionary signatures of B. glumae in the process of switching niches from plants to humans.

Genome sequence and adaptation of strain of the opportunistic pathogen Burkholderia glumae

2020 ◽  
Author(s):  
Zhouqi Cui ◽  
Sai Wang ◽  
K. U. Kakar ◽  
Guanlin Xie ◽  
Bin Li ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Opportunistic Pathogen ◽  
Antibiotic Biosynthesis ◽  
Burkholderia Glumae ◽  
Niche Adaptation ◽  
Niche Diversity ◽  
History Of ◽  
Clinical Conditions ◽  
Shed Light ◽  
Important Disease

Abstract Background: Burkholderia glumae causes rice (Oryza sativa) bacterial panicle blight, which is an increasingly economically important disease worldwide. B. glumae can also be isolated from humans who suffer from chronic granulomatous disease. However, our understanding of the molecular mechanism underlying human pathogenesis by B. glumae remains rudimentary. Results: In this study, we report the complete genome sequence of the human-isolated B. glumae strain AU6208 and compare this to the genome of the plant-pathogenic B. glumae type strain LMG 2196T, which was isolated from rice. Analysis of the gene content and average nucleotide identity demonstrated 99.4% similarity between the human- and plant-pathogenic strains. However, the phenotypic results from this study suggest a history of niche adaptation and divergence. For example, AU6208 produces more exopolysaccharides and more readily forms biofilms compared with LMG 2196T. Consistent with this, the AU6208 genome encodes a UDP-galactopyranose mutase (BCAL3235), but LMG 2196T lacks this gene. In total, 44 genes were predicted to be horizontally transferred into AU6208, and most of these genes were upregulated in conditions that mimic clinical conditions. Among the horizontally transferred genes, the gene pair SbnABas encodes key enzymes in antibiotic biosynthesis. These results suggest that horizontal gene transfer in AU6208 may be responsible for selective advantages in its pathogenicity in humans. Conclusions: In summary, our analysis of the AU6208 genome and comparison with that of LMG 2196T reveal the evolutionary signatures of B. glumae in the process of switching niches from plants to humans. Our findings may shed light on strain and niche diversity in bacteria.

scholarly journals Genome Sequence Resource of Burkholderia glumae UAPB13

2021 ◽  
Author(s):  
Juanita Gil ◽  
Laura Ortega ◽  
J Alejandro Rojas ◽  
Clemencia M Rojas
Keyword(s):  
Genome Sequence ◽  
Genomic Sequence ◽  
Burkholderia Glumae ◽  
Genomic Complexity ◽  
Panicle Blight

Burkholderia glumae causes Bacterial Panicle Blight of rice. Here, we report the genomic sequence of B. glumae strain UAPB13 isolated from fields in Arkansas. The assembled genome consists of 123 scaffolds totaling 6,504,483 bp representing two chromosomes and two plasmids. The genomic complexity of B. glumae warrants the sequencing of additional strains. This additional genomic sequence will enable us to further understand this pathogen and the disease it causes.

PCR-RFLP Detection od PAI-2 Gene Variants: Prevelence in Ethnic Groups and Disease Relationship in patients Undergoing corony Angiography

1997 ◽  
Vol 77 (05) ◽  
pp. 0955-0958 ◽  
Author(s):  
Carole A Foy ◽  
Peter J Grant
Keyword(s):  
Gene Variants ◽  
Genotype Distribution ◽  
Pima Indians ◽  
Significant Difference ◽  
Pcr Rflp ◽  
History Of ◽  
Caucasian Patients ◽  
Clinical Conditions ◽  
Rflp Assay ◽  
Coronary Atheroma

SummaryPAI-2 is a fibrinolytic inhibitor produced predominantly by monocytes. Most PAI-2 is intracellular making study in clinical conditions difficult. Abnormalities in production may be associated with inflammation and fibrinolysis at sites of tissue damage such as the atherosclerotic plaque.PAI-2 gene variants have been described: variant A consists of Asn120, Asn404 and Ser413 and variant B consists of Asp120, Lys404 and Cys413. We designed a PCR-RFLP assay using primers spanning the region containing Asn/Lys404 and Ser/Cys413. Variant B contains an Mwol restriction site. We analysed 302 Pima Indians and 286 healthy Caucasian volunteers. To investigate relationships between genotype and vascular disease we analysed 333 Caucasian patients undergoing coronary angiography.Gene variant B was more common in the Pimas than in Caucasians (p <0.0001). There was no significant difference in genotype distribution between the volunteers and patients. In the patients there was no association between genotype and either a history of MI or extent of coronary atheroma.

scholarly journals Cancer immunotherapy in special challenging populations: recommendations of the Advisory Committee of Spanish Melanoma Group (GEM)

2021 ◽  
Vol 9 (3) ◽  
pp. e001664
Author(s):  
Maria Gonzalez-Cao ◽  
Teresa Puertolas ◽  
Mar Riveiro ◽  
Eva Muñoz-Couselo ◽  
Carolina Ortiz ◽  
...  
Keyword(s):  
Cancer Immunotherapy ◽  
Multidisciplinary Approach ◽  
Checkpoint Inhibitors ◽  
Solid Organ Transplantation ◽  
Latent Tuberculosis ◽  
Solid Organ ◽  
Chronic Infections ◽  
Immunosuppressed Patients ◽  
History Of ◽  
Evidence Based Guidelines

Cancer immunotherapy based on the use of antibodies targeting the so-called checkpoint inhibitors, such as programmed cell death-1 receptor, its ligand, or CTLA-4, has shown durable clinical benefit and survival improvement in melanoma and other tumors. However, there are some special situations that could be a challenge for clinical management. Persons with chronic infections, such as HIV-1 or viral hepatitis, latent tuberculosis, or a history of solid organ transplantation, could be candidates for cancer immunotherapy, but their management requires a multidisciplinary approach. The Spanish Melanoma Group (GEM) panel in collaboration with experts in virology and immunology from different centers in Spain reviewed the literature and developed evidence-based guidelines for cancer immunotherapy management in patients with chronic infections and immunosuppression. These are the first clinical guidelines for cancer immunotherapy treatment in special challenging populations. Cancer immunotherapy in chronically infected or immunosuppressed patients is feasible but needs a multidisciplinary approach in order to decrease the risk of complications related to the coexistent comorbidities.

scholarly journals Complete genome sequence of Leptotrichia buccalis type strain (C-1013-bT)

2009 ◽  
Vol 1 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Natalia Ivanova ◽  
Sabine Gronow ◽  
Alla Lapidus ◽  
Alex Copeland ◽  
Tijana Glavina Del Rio ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain

scholarly journals Complete genome sequence of the gliding, heparinolytic Pedobacter saltans type strain (113T)

2011 ◽  
Vol 5 (1) ◽  
pp. 30-40 ◽  
Author(s):  
Konstantinos Liolios ◽  
Johannes Sikorski ◽  
Meagan Lu ◽  
Matt Nolan ◽  
Alla Lapidus ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain

Acceptance and Commitment Therapy (ACT): A Third Wave Behaviour Therapy

2008 ◽  
Vol 36 (6) ◽  
pp. 667-673 ◽  
Author(s):  
Chris Cullen
Keyword(s):  
Acceptance And Commitment Therapy ◽  
Relational Frame Theory ◽  
Third Wave ◽  
Behaviour Therapy ◽  
Commitment Therapy ◽  
Acceptance And Commitment ◽  
Relational Frame ◽  
Important Relationship ◽  
History Of ◽  
Clinical Conditions

AbstractThis paper introduces Acceptance and commitment Therapy (ACT) as one of the newer contextualist behaviour therapies. A brief history of the development of ACT is outlined. The concepts of equivalence and laterality and the important relationship between Relational Frame Theory and ACT are then described. The “hexagram” summary of the six core linked processes in ACT is presented and, finally, the research evidence to support the effectiveness of ACT applied to a range of clinical conditions and client groups is summarized.

scholarly journals Complete genome sequence of Terriglobus saanensis type strain SP1PR4T, an Acidobacteria from tundra soil

2012 ◽  
Vol 7 (1) ◽  
pp. 59-69 ◽  
Author(s):  
Suman R. Rawat ◽  
Minna K. Männistö ◽  
Valentin Starovoytov ◽  
Lynne Goodwin ◽  
Matt Nolan ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Tundra Soil

scholarly journals Complete genome sequence of Mesorhizobium australicum type strain (WSM2073T)

2013 ◽  
Vol 9 (2) ◽  
pp. 410-419 ◽  
Author(s):  
Wayne Reeve ◽  
Kemanthi Nandasena ◽  
Ron Yates ◽  
Ravi Tiwari ◽  
Graham O’Hara ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain
Sign in / Sign up

Export Citation Format

Share Document