scholarly journals Low Pathogenic Strain of Infectious Pancreatic Necrosis Virus (IPNV) Associated with Recent Outbreaks in Iranian Trout Farms

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 782
Author(s):  
Sohrab Ahmadivand ◽  
Manfred Weidmann ◽  
Mansour El-Matbouli ◽  
Hooman Rahmati-Holasoo
Keyword(s):  
Pancreatic Necrosis ◽  
Histopathological Examination ◽  
Kidney Tissue ◽  
Clinical Signs ◽  
Abdominal Distension ◽  
Infectious Pancreatic Necrosis Virus ◽  
Gene Encoding ◽  
Vp2 Gene ◽  
Infectious Pancreatic Necrosis ◽  
Trout Farms

Infectious pancreatic necrosis (IPN), first described as acute viral catarrhal enteritis, is a highly contagious disease with variable pathogenicity that has been linked to genetic variation in the viral VP2 gene encoding the capsid protein. In this study, the IPN virus (IPNV) is isolated from the moribund fish from five of fourteen Iranian trout farms from 2015 to 2017. The affected fish showed mortality rates ranging from 20% to 60%, with the main clinical signs of exophthalmia, darkened skin, and mild abdominal distension, as well as yellow mucoid fluid in the intestine. Histopathological examination of intestinal sections confirmed acute catarrhal enteritis in all samples. RT-PCR assay of the kidney tissue and cell culture (CHSE-214) samples consistently confirmed the presence of the virus. The phylogenetic analysis of the partial VP2 sequence revealed that the detected isolates belong to genogroup 5, and are closely related to the Sp serotype strains of European origin. Characterization of VP2 of all isolates revealed the P217T221 motif that previously was associated with avirulence or low virulence, while all IPNV-positive fish in this study were clinically affected with moderate mortality. The IPNV isolates from Iran are associated with two lineages that appear to have originated from Europe, possibly via imported eggs.

Infectious Pancreatic Necrosis Virus Occurrence at a Hatchery in Alberta

1974 ◽  
Vol 31 (4) ◽  
pp. 397-402 ◽  
Author(s):  
T. Yamamoto
Keyword(s):  
Brook Trout ◽  
Pancreatic Necrosis ◽  
Salvelinus Fontinalis ◽  
Kidney Tissue ◽  
Infectious Pancreatic Necrosis Virus ◽  
Salmo Gairdneri ◽  
Necrosis Virus ◽  
High Incidence ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

The presence of infectious pancreatic necrosis (IPN) virus in brook (Salvelinus fontinalis) and rainbow (Salmo gairdneri) trout was identified and further investigated. A high incidence of virus was found by virological examination of kidney tissue from yearling and older trout. By comparison, brook trout had a much higher incidence of virus than rainbow trout.The virus was isolated most frequently from the kidneys of both species of trout. These findings indicate that the examinations of peritoneal washes and feces is not adequate for the detection of IPN carrier fish.

Infectious pancreatic necrosis virus (IPNV) serotype Sp is prevalent in Turkish rainbow trout farms

2017 ◽  
Vol 41 (1) ◽  
pp. 95-104 ◽  
Author(s):  
A G Büyükekiz ◽  
S Altun ◽  
E F Hansen ◽  
İ B Satıcıoğlu ◽  
M Duman ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus ◽  
Trout Farms

scholarly journals Molecular Evolution of Infectious Pancreatic Necrosis Virus in China

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 488
Author(s):  
Kaiyue Duan ◽  
Jingzhuang Zhao ◽  
Guangming Ren ◽  
Yizhi Shao ◽  
Tongyan Lu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Molecular Evolution ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Amino Acid Sequences ◽  
Necrosis Virus ◽  
Vp2 Gene ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus ◽  
Highest Posterior Density

Passive virus surveillance was performed in twenty-nine salmon and trout farms from seven provinces and districts in China during the period 2017–2020. A total of 25 infectious pancreatic necrosis virus (IPNV) isolates were obtained, mainly from rainbow trout (Oncorhynchus mykiss). The molecular evolution of these Chinese IPNV isolates and the previously reported Chinese IPNV strains ChRtm213 and WZ2016 was analyzed, based on their VP2 gene coding region sequences (CDS). All 27 Chinese IPNV isolates clustered within genogroups I and V, with 24 of the IPNV isolates belonging to genogroup I (including ChRtm213 and WZ2016), and only three isolates clustering in genogroup V. The Chinese genogroup I IPNV isolates lacked diversity, composing six haplotypes with 41 polymorphic sites, and the identity of nucleotide and amino acid sequences among the entire VP2 gene CDS from these isolates was 97.44%–100% and 98.19%–100%, respectively. Divergence time analyses revealed that the Chinese genogroup I IPNV isolates likely diverged from Japanese IPNV isolates in 1985 (95% highest posterior density (HPD), 1965–1997), and diverged again in 2006 (95% HPD, 1996–2013) in China. Each of the three Chinese genogroup V IPNV isolates has a unique VP2 gene CDS, with a total of 21 polymorphic sites; the identity of nucleotide and amino acid sequences among all VP2 gene CDS from these isolates was 98.5%–99.5% and 98.6%–99.0%, respectively. The data demonstrate that genogroups I and V are more likely the currently prevalent Chinese IPNV genotypes.

Sign in / Sign up

Export Citation Format

Share Document