Stable Luminescent Poly(Allylaminehydrochloride)-Templated Copper Nanoclusters for Selectively Turn-Off Sensing of Deferasirox in β-Thalassemia Plasma

Highly stable and facile one-pot copper nanoclusters (Cu NCs) coated with poly(allylamine hydrochloride) (PAH) have been synthesized for selectively sensing deferasirox (DFX) in β-thalassemia plasma. DFX is an important drug used for treating iron overloading in β-thalassemia, but needs to be monitored due to certain toxicity. In this study, the PAH-Cu NCs showed highly stable fluorescence with emission wavelengths at 450 nm. The DFX specifically interacted with the copper nanocluster to turn off the fluorescence of the PAH-Cu NCs, and could be selectively quantified through the fluorescence quenching effect. The linear range of DFX in plasma analyzed by PAH-Cu NCs was 1.0–100.0 µg/mL (r = 0.985). The relative standard deviation (RSD) and relative error (RE) were lower than 6.51% and 7.57%, respectively, showing excellent reproducibility of PAH-Cu NCs for sensing DFX in plasma. This method was also successfully applied for an analysis of three clinical plasma samples from β-thalassemia patients taking DFX. The data presented high similarity with that obtained through a capillary electrophoresis method. According to the results, the PAH-Cu NCs could be used as a tool for clinically sensing DFX in human plasma for clinical surveys.

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Capillary Electrophoresis Method for Fexofenadine Hydrochloride in Capsules

Journal of AOAC International ◽

10.1093/jaoac/88.4.1059 ◽

2005 ◽

Vol 88 (4) ◽

pp. 1059-1063 ◽

Cited By ~ 14

Author(s):

Ana R Breier ◽

Sílvia S Garcia ◽

André Jablonski ◽

Martin Steppe ◽

Elfrides E S Schapoval

Keyword(s):

Capillary Electrophoresis ◽

Potential Temperature ◽

Fused Silica ◽

System Control ◽

Chromatography Method ◽

Limits Of Detection ◽

Capillary Electrophoresis Method ◽

Relative Standard ◽

Significant Difference ◽

Electrophoresis Method

Abstract A simple, accurate, and effective capillary electrophoresis method with ultraviolet absorbance detection was developed and validated for the quantitation of the antihistamine fexofenadine in capsules. The separation was performed with an uncoated fused-silica capillary (47 cm × 75 μm id) and was operated at 20 kV potential. Temperature was maintained at 25°C. The run buffer was prepared with 20mM Na2B4O7 × 10 H2O. Software was used for system control, data acquisition, and analysis. Method validation was performed by evaluation of the analytical parameters linearity, precision, accuracy, limits of detection and quantitation, and specificity. The method was linear (r = 0.9999) at concentrations ranging from 20 to 100 μg/mL, precise (relative standard deviation intra-assay = 1.2, 1.6, and 1.8% and interassay = 1.5%); accurate (recovery = 98.1%); and specific. The limits of detection and quantitation were 0.69 and 2.09 μg/mL, respectively. The method was compared to the liquid chromatography method developed previously by the authors for the same drug, and no significant difference was found between the 2 methods in fexofenadine hydrochloride quantitation.

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Development of a capillary electrophoresis method for the simultaneous determination of cephalosporins

Journal of the Serbian Chemical Society ◽

10.2298/jsc121117028h ◽

2013 ◽

Vol 78 (9) ◽

pp. 1413-1423 ◽

Cited By ~ 10

Author(s):

Gabriel Hancu ◽

Hajnal Kelemen ◽

Aura Rusu ◽

Árpád Gyéresi

Keyword(s):

Capillary Electrophoresis ◽

Simultaneous Determination ◽

Sodium Dodecyl ◽

Buffer Solution ◽

Capillary Electrophoresis Method ◽

Relative Standard ◽

Peak Areas ◽

Electrophoresis Method ◽

Cephalosporin Antibiotics

A rapid and simple capillary electrophoresis method has been developed for the simultaneous determination of six extensively used cephalosporin antibiotics (cefaclor, cefadroxil, cefalexin, cefuroxim, ceftazidim, ceftriaxon). The determination of cephalosporins was performed at a pH 6.8, using a 25 mM phospate - 25 mM borate mixed buffer, + 25 kV voltage at a temperature of 25 ?C. We achieved a baseline separation in approximately 10 minutes. The separation resolution was increased by addition of an anionic surfactant, 50 mM sodium dodecyl sulfate, to the buffer solution. The proposed separation was evaluated on the basis of detection and quantification limits, effective electrophoretic mobility and relative standard deviation for migration times and peak areas.

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Validation of a Capillary Electrophoresis Method for Analysis of Rabeprazole Sodium in a Pharmaceutical Dosage Form

Journal of AOAC International ◽

10.1093/jaoac/88.4.1081 ◽

2005 ◽

Vol 88 (4) ◽

pp. 1081-1085 ◽

Cited By ~ 11

Author(s):

Cássia V Garcia ◽

Juliana Sippel ◽

Leticia L Sfair ◽

Silvia S Garcia ◽

André Jablonski ◽

...

Keyword(s):

Capillary Electrophoresis ◽

Fused Silica ◽

Array Detector ◽

System Control ◽

Fast Analysis ◽

Pharmaceutical Dosage Form ◽

Capillary Electrophoresis Method ◽

Relative Standard ◽

Rabeprazole Sodium ◽

Electrophoresis Method

Abstract Rabeprazole sodium is an antisecretory agent that inhibits the enzyme H+/K+ ATPase present in the stomach parietal cells. There are few data about its quantitative determinations in laboratorial routines. Capillary electrophoresis is a method being used increasingly for analysis of pharmaceutical compounds, the main advantages of which are the simplicity of instrumentation, low consumption of sample and reagents, and fast analysis. The aim of this study was to develop and validate a capillary electrophoresis method for determination of rabeprazole sodium in coated tablets. The conditions used were a bare fused silica capillary with 48.0 cm length (39.5 cm effective) and 75 μm id; a 10mM, pH 9.0, sodium tetraborate run buffer; a diode array detector set at 291 nm; hydrodynamic injection (50 mbar/5 s); and a voltage of 20 kV. HP Chemstation CE rev. A.06.03 software was used for system control, data acquisition, and analysis. The method was demonstrated to be linear in the concentration range of 5.0–40.0 μg/mL (r = 0.9993), precise (interday relative standard deviation = 0.49), accurate (mean recovery = 103.1%), and specific. The limits of detection and quantitation were 1.29 and 3.91 μg/mL, respectively.

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Study on development of an analytical procedure to quantify glyphosate by capillary electrophoresis method and its application for beverage

Ministry of Science and Technology, Vietnam ◽

10.31276/vjst.63(11).58-64 ◽

2021 ◽

Vol 63 (11) ◽

pp. 58-64

Author(s):

Manh Huy Nguyen ◽

◽

Thi Ha Tran ◽

Minh Tuan Vu ◽

Thanh Dam Nguyen ◽

...

Keyword(s):

Acetic Acid ◽

Capillary Electrophoresis ◽

Analytical Procedure ◽

Ph Value ◽

Solid Phase ◽

Residue Analysis ◽

Background Electrolyte ◽

Capillary Electrophoresis Method ◽

Relative Standard ◽

Electrophoresis Method

Nowadays, the issue of fresh food, drinks, and residue analysis of toxic compounds in food and drinks is increasingly interested in society. In this study, an analytical procedure for quantitative analysis of the glyphosate residue in beverages such as tea infusion and beer was developed based on the capillary electrophoresis method with capacitively-coupled contactless conductivity detector combined with sample preparation using solid phase extraction technique. The analytical conditions optimised for capillary electrophoresis equipment included: histidine/acetic acid background electrolyte solution with histidine concentration of 1 mM and pH value was adjusted to 2.75 by acetic acid; a separation voltage was 20 kV was applied and a high voltage injection at 20 kV for 10 seconds was chosen. The optimised analytical procedure has resulted in a low detection limit of glyphosate (0.42 μg/l), the repeatability and reproducibility expressed by the relative standard deviation of the peak area and migration time were both less than 10%, a wide linear range, and the obtained recovery efficiency of glyphosate on different drinks were achieved to values ranging from 88.7 to 96.3%.

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