Molecular Dissection of Cucumis metuliferus Resistance against Papaya Ringspot Virus by Grafting

Vegetable crops of the genus Cucumis are very popular worldwide and have great market value. However, their fruit quality and yield are hindered by viral diseases. C. metuliferus is considered a wild species with resistance to viral diseases that is lacking in cultivated crops of the Cucumis genus, such as melon. The C. metuliferus line L37 shows extreme resistance against Papaya ringspot virus (PRSV-HA), whereas line L35 is a susceptible line. In this study, reciprocal grafting experiments between L35 and L37 were performed, and the PRSV-HA strain was pre-inoculated in the rootstock leaves. The results revealed that the resistance signal in the L37 rootstock could transmit and provide resistance to the L35 scion. Subsequently, double sandwich grafting was performed using the pre-inoculated L35 as the rootstock, which was then grafted onto the L37 intermediate and the L35 scion. The results showed that PRSV-HA RNA accumulated in the L35 rootstock leaf, petiole, and stem tissues, whereas PRSV-HA RNA accumulated in some intermediate and scion petiole and stem tissues. No HCPro RNA was detected in the L35 scion leaves. The results showed that the suppression of the virus occurred in the leaves, and the resistance effect spread from the rootstock in the scion direction. Hence, this study has demonstrated that RNA silencing of systemic signals is responsible for L37 resistance against PRSV. C. metuliferus L37 could provide a valuable resistance source for crops of the Cucumis species against viral diseases through grafting.

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Cross protection against the watermelon strain of Papaya ringspot virus through modification of viral RNA silencing suppressor

Virus Research ◽

10.1016/j.virusres.2019.03.016 ◽

2019 ◽

Vol 265 ◽

pp. 166-171 ◽

Cited By ~ 5

Author(s):

Xian-De Huang ◽

Le Fang ◽

Qin-Sheng Gu ◽

Yan-Ping Tian ◽

Chao Geng ◽

...

Keyword(s):

Rna Silencing ◽

Cross Protection ◽

Ringspot Virus ◽

Viral Rna ◽

Papaya Ringspot Virus ◽

Silencing Suppressor ◽

Rna Silencing Suppressor

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Complete Genome Sequence of Papaya Ringspot Virus Isolated from Genetically Modified Papaya in Hainan Island, China

Genome Announcements ◽

10.1128/genomea.01056-15 ◽

2015 ◽

Vol 3 (5) ◽

Cited By ~ 2

Author(s):

Guangyuan Zhao ◽

Pu Yan ◽

Wentao Shen ◽

Decai Tuo ◽

Xiaoying Li ◽

...

Keyword(s):

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Virus Isolate ◽

Genetically Modified ◽

Hainan Island ◽

Ringspot Virus ◽

Papaya Ringspot Virus ◽

Rt Pcr ◽

Sequence Identity

The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya.

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Screening the Watermelon Germplasm Collection for Resistance to Papaya Ringspot Virus Type‐W

Crop Science ◽

10.2135/cropsci2002.1324 ◽

2002 ◽

Vol 42 (4) ◽

pp. 1324-1330 ◽

Cited By ~ 18

Author(s):

E. Bruton Strange ◽

Nihat Guner ◽

Zvezdana Pesic‐VanEsbroeck ◽

Todd C. Wehner

Keyword(s):

Virus Type ◽

Germplasm Collection ◽

Ringspot Virus ◽

Papaya Ringspot Virus

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Erratum to: Molecular characterization of a severe isolate of papaya ringspot virus in Mexico and its relationship with other isolates

Virus Genes ◽

10.1007/s11262-007-0099-7 ◽

2007 ◽

Vol 35 (2) ◽

pp. 431-431

Author(s):

Juan Carlos Noa-Carrazana ◽

Diego González-de-León ◽

Laura Silva-Rosales

Keyword(s):

Molecular Characterization ◽

Ringspot Virus ◽

Papaya Ringspot Virus ◽

Severe Isolate

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Transgene-specific and event-specific molecular markers for characterization of transgenic papaya lines resistant to Papaya ringspot virus

Transgenic Research ◽

10.1007/s11248-009-9287-7 ◽

2009 ◽

Vol 18 (6) ◽

pp. 971-986 ◽

Cited By ~ 18

Author(s):

Ming-Jen Fan ◽

Shu Chen ◽

Yi-Jung Kung ◽

Ying-Huey Cheng ◽

Huey-Jiunn Bau ◽

...

Keyword(s):

Molecular Markers ◽

Ringspot Virus ◽

Papaya Ringspot Virus ◽

Transgenic Papaya

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Generation of mild recombinants of papaya ringspot virus to minimize the problem of strain-specific cross protection

Phytopathology ◽

10.1094/phyto-06-21-0272-r ◽

2021 ◽

Author(s):

Thi-Thu-Yen Tran ◽

Tzu-Tung Lin ◽

Chung-Ping Chang ◽

Chun-Hung Chen ◽

Van-Hoa Nguyen ◽

...

Keyword(s):

Nitrous Acid ◽

Carica Papaya ◽

Genomic Sequence ◽

Cross Protection ◽

Ringspot Virus ◽

Limiting Factor ◽

Papaya Ringspot Virus ◽

Severe Damage ◽

Mild Strain ◽

Geographic Regions

Papaya ringspot virus (PRSV) causes severe damage to papaya (Carica papaya L.) and is the primary limiting factor for papaya production worldwide. A nitrous acid-induced mild strain PRSV HA 5-1, derived from Hawaii strain HA, has been applied to control PRSV by cross protection for decades. However, the problem of strain-specific protection hampers its application in Taiwan and other geographic regions outside Hawaii. Here, sequence comparison of the genomic sequence of HA 5-1 with that of HA revealed 69 nucleotide changes, resulting in 31 aa changes in which 16 aa are structurally different. The multiple mutations of HA 5-1 are considered resulting from nitrous-acid induction since 86% of nucleotide changes are transition mutations. The stable HA 5-1 was used as a backbone to generate recombinants carrying individual 3’ fragments of Vietnam severe strain TG5, including NIa, NIb, and CP3’ regions, individually or in combination. Our results indicated that the best heterologous fragment for the recombinant is the region of CP3’, with which symptom attenuation of the recombinant is like that of HA 5-1. This mild recombinant HA51/TG5-CP3’ retained high levels of protection against the homologous HA in papaya plants and significantly increased the protection against the heterologous TG-5. Similarly, HA 5-1 recombinants carrying individual CP3’ fragments from Thailand SMK, Taiwan YK, and Vietnam ST2 severe strains also significantly increase the protection against the corresponding heterologous strains in papaya plants. Thus, our recombinant approach for mild strain generation is a fast and effective way to minimize the problem of strain-specific protection.

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Danos causados pelo Zucchini lethal chlorosis virus (ZLCV) sobre a produção de frutos comerciais de abobrinha de moita 'Caserta'

Summa Phytopathologica ◽

10.1590/s0100-54052009000300011 ◽

2009 ◽

Vol 35 (3) ◽

pp. 223-225 ◽

Cited By ~ 7

Author(s):

José Segundo Giampan ◽

Jorge Alberto Marques Rezende ◽

Sônia Maria De Stefano Piedade

Keyword(s):

Mosaic Virus ◽

Virus Type ◽

Zucchini Yellow Mosaic Virus ◽

Ringspot Virus ◽

Yellow Mosaic Virus ◽

Papaya Ringspot Virus

O ZLCV é um tospovírus encontrado com freqüência causando severos danos em cucurbitáceas. Nesse trabalho avaliaram-se os danos causados pelo ZLCV em abobrinha de moita 'Caserta', em campo na ESALQ/USP, Piracicaba-SP, onde esse vírus é freqüente. Plantas obtidas pela semeadura direta foram monitoradas periodicamente quanto à infecção pelo ZLCV por meio dos sintomas e por PTA-ELISA. Monitorou-se ainda a contaminação com Papaya ringspot virus - type W e Zucchini yellow mosaic virus, desconsiderando a produção dessas plantas. As plantas foram agrupadas em função da época de aparecimento dos sintomas do ZLCV, avaliando a produção de frutos comerciais (FC) e não comerciais (FNC) de cada grupo e comparando com a de plantas que permaneceram sem sintomas até o final do experimento. As plantas que apresentaram sintomas até os 23 dias após a emergência (DAE) não produziram qualquer tipo de frutos. FC foram colhidos de plantas que apresentaram sintomas a partir dos 42 DAE. Mesmo assim, houve redução de 78,5 % na produção de FC. Plantas que mostraram sintomas por ocasião da última colheita (55 DAE) apresentaram redução na produção de FC de 9,6 %. A infecção com o ZLCV até o início da frutificação inviabiliza a produção de FC de abobrinha de moita 'Caserta'.

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Cymbidium Ringspot Virus Harnesses RNA Silencing To Control the Accumulation of Virus Parasite Satellite RNA

Journal of Virology ◽

10.1128/jvi.01343-08 ◽

2008 ◽

Vol 82 (23) ◽

pp. 11851-11858 ◽

Cited By ~ 19

Author(s):

Vitantonio Pantaleo ◽

József Burgyán

Keyword(s):

Rna Silencing ◽

Sequence Similarity ◽

Helper Virus ◽

Ringspot Virus ◽

Silencing Suppressor ◽

Short Interfering Rna ◽

Satellite Rna ◽

The Cost ◽

Interfering Rna ◽

Rna Replicon

ABSTRACT Cymbidium ringspot virus (CymRSV) satellite RNA (satRNA) is a parasitic subviral RNA replicon that replicates and accumulates at the cost of its helper virus. This 621-nucleotide (nt) satRNA species has no sequence similarity to the helper virus, except for a 51-nt-long region termed the helper-satellite homology (HSH) region, which is essential for satRNA replication. We show that the accumulation of satRNA strongly depends on temperature and on the presence of the helper virus p19 silencing suppressor protein, suggesting that RNA silencing plays a crucial role in satRNA accumulation. We also demonstrate that another member of the Tombusvirus genus, Carnation Italian ringspot virus (CIRV), supports satRNA accumulation at a higher level than CymRSV. Our results suggest that short interfering RNA (siRNA) derived from CymRSV targets satRNA more efficiently than siRNA from CIRV, possibly because of the higher sequence similarity between the HSH regions of the helper and CIRV satRNAs. RNA silencing sensor RNA carrying the putative satRNA target site in the HSH region was efficiently cleaved when transiently expressed in CymRSV-infected plants but not in CIRV-infected plants. Strikingly, replacing the CymRSV HSH box2 sequence with that of CIRV restores satRNA accumulation both at 24°C and in the absence of the p19 suppressor protein. These findings demonstrate the extraordinary adaptation of this virus to its host in terms of harnessing the antiviral silencing response of the plant to control the virus parasite satRNA.

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Colored shading nets impede insect invasion and decrease the incidences of insect-transmitted viral diseases in vegetable crops

Entomologia Experimentalis et Applicata ◽

10.1111/j.1570-7458.2012.01293.x ◽

2012 ◽

Vol 144 (3) ◽

pp. 249-257 ◽

Cited By ~ 30

Author(s):

David Ben-Yakir ◽

Yehezkel Antignus ◽

Yossi Offir ◽

Yosepha Shahak

Keyword(s):

Viral Diseases ◽

Vegetable Crops

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Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus

Scientia Agricola ◽

10.1590/s0103-90162003000400013 ◽

2003 ◽

Vol 60 (4) ◽

pp. 691-698 ◽

Cited By ~ 8

Author(s):

Davi Andrade Pacheco ◽

Jorge Alberto Marques Rezende ◽

Sônia Maria de Stefano Piedade

Keyword(s):

Virus Type ◽

Aerial Part ◽

Mosaic Disease ◽

Ringspot Virus ◽

Virus Concentration ◽

Papaya Ringspot Virus ◽

Severe Strain ◽

Dry Biomass ◽

Marketable Fruit ◽

Cotyledonary Stage

Pre-immunization with mild strains of Papaya ringspot virus - type W (PRWV-W) has allowed the mosaic disease to be controlled in different cucurbit species, with increases in marketable fruit yield. The objective of this study was to compare virus concentration, biomass and symptomatology of 'Caserta' zucchini squash, 'Menina Brasileira' long-neck squash and 'Crimson Sweet' watermelon plants infected by three mild strains and one severe strain of PRSV-W. Plants were inoculated at the cotyledonary stage, under greenhouse conditions, sampled at 7, 14, 21, 28 and 35 days after inoculation (DAI), and analyzed by PTA-ELISA. The severity of the symptoms was scored according to a scale from 1 to 5, and the fresh and dry biomass of the aerial part of the plants were evaluated at 40 DAI. Concentrations of the mild strains, based on absorbance values of the PTA-ELISA, were lower than the concentration of the severe strain for all species. The mild strains did not cause mosaic in infected plants of all species. Plants of zucchini squash and watermelon infected by the severe strain exhibited severe mosaic symptoms, but the same was not noticed for infected long-neck squash plants. Biomass values from zucchini squash and watermelon plants infected by the mild strains were 1.7 % to 12.4 % lower as compared to healthy plants. Biomass values of zucchini squash and watermelon plants infected by the severe strain presented greater reduction, varying from 29 % to 74 %. However, biomass values of long-neck squash plants infected by the mild and severe strains were similar for all treatments.

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