scholarly journals Fiber Optic Particle Plasmon Resonance-Based Immunoassay Using a Novel Multi-Microchannel Biochip

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3086
Author(s):  
Chang-Yue Chiang ◽  
Chien-Hsing Chen ◽  
Chien-Tsung Wang

A novel multi-microchannel biochip fiber-optic particle plasmon resonance (FOPPR) sensor system for the simultaneous detection of multiple samples. The system integrates a novel photoelectric system, a lock-in module, and an all-in-one platform incorporating optical design and mechanical design together to improve system stability and the sensitivity of the FOPPR sensor. The multi-microchannel FOPPR biochip has been developed by constructing a multi-microchannel flow-cell composed of plastic material to monitor and analyze five samples simultaneously. The sensor system requires only 30 μL of sample for detection in each microchannel. Moreover, the total size of the multi-microchannel FOPPR sensor chip is merely 40 mm × 30 mm × 4 mm; thus, it is very compact and cost-effective. The analysis was based on calibration curves obtained from real-time sensor response data after injection of sucrose solution, streptavidin and anti-dinitrophenyl (anti-DNP) antibody of known concentrations over the chips. The results show that the multi-microchannel FOPPR sensor system not only has good reproducibility (coefficient of variation (CV) < 10%), but also excellent refractive index resolution (6.23 ± 0.10 × 10−6 refractive index unit (RIU)). The detection limits are 2.92 ± 0.28 × 10−8 g/mL (0.53 ± 0.01 nM) and 7.48 ± 0.40 × 10−8 g/mL (0.34 ± 0.002 nM) for streptavidin and anti-DNP antibody, respectively.

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hyeong-Min Kim ◽  
Jae-Hyoung Park ◽  
Seung-Ki Lee

Abstract Fiber-optic-based localized surface plasmon resonance (FO-LSPR) sensors with three-dimensional (3D) nanostructures have been developed. These sensors were fabricated using zinc oxide (ZnO) nanowires and gold nanoparticles (AuNPs) for highly sensitive plasmonic biosensing. The main achievements in the development of the biosensors include: (1) an extended sensing area, (2) light trapping effect by nanowires, and (3) a simple optical system based on an optical fiber. The 3D nanostructure was fabricated by growing the ZnO nanowires on the cross-section of optical fibers using hydrothermal synthesis and via immobilization of AuNPs on the nanowires. The proposed sensor outputted a linear response according to refractive index changes. The 3D FO-LSPR sensor exhibited an enhanced localized surface plasmon resonance response of 171% for bulk refractive index changes when compared to the two-dimensional (2D) FO-LSPR sensors where the AuNPs are fixed on optical fiber as a monolayer. In addition, the prostate-specific antigen known as a useful biomarker to diagnose prostate cancer was measured with various concentrations in 2D and 3D FO-LSPR sensors, and the limits of detection (LODs) were 2.06 and 0.51 pg/ml, respectively. When compared to the 2D nanostructure, the LOD of the sensor with 3D nanostructure was increased by 404%.


2020 ◽  
Vol 157 ◽  
pp. 105026 ◽  
Author(s):  
Ting-Chou Chang ◽  
Chang-Yue Chiang ◽  
Min-Hsiu Lin ◽  
I-Kuang Chen ◽  
Lai-Kwan Chau ◽  
...  

2015 ◽  
Vol 44 (2) ◽  
pp. 228001
Author(s):  
付丽辉 FU Li-hui ◽  
尹文庆 YIN Wen-qing ◽  
王马华 WANG Ma-hua ◽  
季仁东 JI Ren-dong ◽  
居勇峰 JU Yong-feng

Sign in / Sign up

Export Citation Format

Share Document