scholarly journals Hemp Chemotype Definition by Cannabinoids Characterization Using LC-ESI(+)-LTQ-FTICR MS and Infrared Multiphoton Dissociation

Separations ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 245
Author(s):  
Filomena Lelario ◽  
Raffaella Pascale ◽  
Giuliana Bianco ◽  
Laura Scrano ◽  
Sabino Aurelio Bufo
Keyword(s):  
Cannabis Sativa ◽  
Ion Trap ◽  
Structural Information ◽  
Mass Measurement ◽  
Infrared Multiphoton Dissociation ◽  
Ion Cyclotron Resonance ◽  
Fragmentation Pattern ◽  
Fticr Ms ◽  
Product Ions ◽  
Multiphoton Dissociation

The development and application of advanced analytical methods for a comprehensive analysis of Cannabis sativa L. extracts plays a pivotal role in order to have a reliable evaluation of their chemotype definition to guarantee the efficacy and safety in pharmaceutical use. This paper deals with the qualitative and quantitative determination of cannabidiol (CBD), tetrahydrocannabinol (THC), cannabinol (CBN), tetrahydrocannabivarin (THCV), cannabidivarin (CBDV), and cannabigerol (CBG) based on a liquid chromategraphy-mass spectrometry (LC-MS) method using electrospray ionization in positive mode (ESI+), coupled with a hybrid quadrupole linear ion trap (LTQ) and Fourier transform ion cyclotron resonance mass spectrometer (FTICR-MS). For the first time, structural information of phytocannabinoids is available upon precursor ions’ isolation within the FTICR trapping cell and subsequent fragmentation induced by infrared multiphoton dissociation (IRMPD). Such fragmentation and accurate mass measurement of product ions, alongside collision-induced dissociation (CID) within LTQ, was advantageous to propose a reliable fragmentation pattern for each compound. Then, the proposed LC-ESI(+)-LTQ-FTICR MS method was successfully applied to the hemp chemotype definition of three registered Italian accessions of hemp C. sativa plants (Carmagnola C.S., Carmagnola, and Eletta Campana), thus resulting in the Eletta Campana accession being the best one for cannabis product manufacturing.

Amplification of Infrared Multiphoton Dissociation Efficiency in a Quadruple Ion Trap Using IR-Active Ligands

2006 ◽  
Vol 78 (24) ◽  
pp. 8512-8517 ◽  
Author(s):  
Michael Pikulski ◽  
Jeffrey J. Wilson ◽  
Apolonio Aguilar ◽  
Jennifer S. Brodbelt
Keyword(s):  
Ion Trap ◽  
Infrared Multiphoton Dissociation ◽  
Dissociation Efficiency ◽  
Multiphoton Dissociation

Structural Characterization of Complex Bacterial Glycolipids by Fourier Transform Mass Spectrometry

2005 ◽  
Vol 11 (5) ◽  
pp. 535-546 ◽  
Author(s):  
Anna Kondakov ◽  
Buko Lindner
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Structural Information ◽  
Adaptive Immune System ◽  
Ion Cyclotron Resonance ◽  
Bacterial Membranes ◽  
Multiphoton Dissociation ◽  
The One ◽  
And Function

Bacterial glycolipids are complex amphiphilic molecules which are, on the one hand, of utmost importance for the organization and function of bacterial membranes and which, on the other hand, play a major role in the activation of cells of the innate and adaptive immune system of the host. Already small alterations to their chemical structure may influence the biological activity tremendously. Due to their intrinsic biological heterogeneity [number and type of fatty acids, saccharide structures and substitution with for example, phosphate ( P), 2-aminoethyl-(pyro)phosphate groups ( P-Etn) or 4-amino-4-deoxyarabinose (Ara4N)], separation of the different components are a prerequisite for unequivocal chemical and nuclear magnetic resonance structural analyses. In this contribution, the structural information which can be obtained from heterogenous samples of glycolipids by Fourier transform (FT) ion cyclotron resonance mass spectrometric methods is described. By means of recently analysed complex biological samples, the possibilities of high-resolution electrospray ionization FT-MS are demonstrated. Capillary skimmer dissociation, as well as tandem mass spectrometry (MS/MS) analysis utilizing collision-induced dissociation and infrared multiphoton dissociation, are compared and their advantages in providing structural information of diagnostic importance are discussed.

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