Insect Body Defence Reactions against Bee Venom: Do Adipokinetic Hormones Play a Role?

Bees originally developed their stinging apparatus and venom against members of their own species from other hives or against predatory insects. Nevertheless, the biological and biochemical response of arthropods to bee venom is not well studied. Thus, in this study, the physiological responses of a model insect species (American cockroach, Periplaneta americana) to honeybee venom were investigated. Bee venom toxins elicited severe stress (LD50 = 1.063 uL venom) resulting in a significant increase in adipokinetic hormones (AKHs) in the cockroach central nervous system and haemolymph. Venom treatment induced a large destruction of muscle cell ultrastructure, especially myofibrils and sarcomeres. Interestingly, co-application of venom with cockroach Peram-CAH-II AKH eliminated this effect. Envenomation modulated the levels of carbohydrates, lipids, and proteins in the haemolymph and the activity of digestive amylases, lipases, and proteases in the midgut. Bee venom significantly reduced vitellogenin levels in females. Dopamine and glutathione (GSH and GSSG) insignificantly increased after venom treatment. However, dopamine levels significantly increased after Peram-CAH-II application and after co-application with bee venom, while GSH and GSSG levels immediately increased after co-application. The results suggest a general reaction of the cockroach body to bee venom and at least a partial involvement of AKHs.

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Orthoptera and allies in the Maritime provinces, Canada: new records and updated provincial checklists

The Canadian Field-Naturalist ◽

10.22621/cfn.v132i4.1984 ◽

2019 ◽

Vol 132 (4) ◽

pp. 319-329 ◽

Cited By ~ 1

Author(s):

John Klymko ◽

Paul Catling ◽

Jeffrey B. Ogden ◽

Robert W. Harding ◽

Donald F. McAlpine ◽

...

Keyword(s):

Prince Edward Island ◽

Native Species ◽

Periplaneta Americana ◽

New Records ◽

American Cockroach ◽

Maritime Provinces ◽

Praying Mantis ◽

Mole Cricket ◽

Oecanthus Nigricornis ◽

Periplaneta Australasiae

We provide an updated checklist of Orthoptera and their allies for each Maritime province of Canada with details for 21 new species records. Drumming Katydid (Meconema thalassinum), recorded from Nova Scotia (NS) and Prince Edward Island (PEI), and Sprinkled Grasshopper (Chloealtis conspersa), recorded from New Brunswick (NB) are reported for the first time from the Maritimes as a whole. We report range extensions in the Maritime region for Australian Cockroach (Periplaneta australasiae; NB), Treetop Bush Katydid (Scudderia fasciata; NS), Short-legged Camel Cricket (Ceuthophilus brevipes; PEI), Spotted Camel Cricket (Ceuthophilus maculatus; PEI), Roesel’s Shield-backed Katydid (Roeseliana roesellii; NS), and Black-horned Tree Cricket (Oecanthus nigricornis; PEI). Short-winged Mole Cricket (Neoscapteriscus abbreviatus; NB) and European Mole Cricket (Gryllotalpa gryllotalpa; NS) are reported as adventives (non-native species that are believed to be not yet established), new to Canada from the Maritimes. Other new records for species not known to be established are Lined Earwig (Doru taeniatum; NS), Australian Cockroach (Periplaneta australasiae; PEI), American Cockroach (Periplaneta americana; NB), Brown Cockroach (Periplaneta brunnea; PEI), Smooth Cockroach (Nyctibora laevigata; NB), West Indian Leaf Cockroach (Blaberus discoidalis; NB), an unidentified Parcoblatta species (NB), Brown-banded Cockroach (Supella longipalpa; PEI), Praying Mantis (Mantis religiosa; NB), and American Bird Grasshopper (Schistocerca americana; NS).

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Cholesterol turnover in the American cockroach, Periplaneta americana (L.)

The Journal of Lipid Research ◽

10.1016/s0022-2275(20)40215-9 ◽

1964 ◽

Vol 5 (3) ◽

pp. 418-421

Author(s):

Hugh E. Vroman ◽

J.N. Kaplanis ◽

W.E. Robbins

Keyword(s):

Periplaneta Americana ◽

American Cockroach

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Molecular cloning of cDNA for lipopolysaccharide-binding protein from the hemolymph of the American cockroach, Periplaneta americana. Similarity of the protein with animal lectins and its acute phase expression

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)98841-1 ◽

1991 ◽

Vol 266 (20) ◽

pp. 13318-13323

Author(s):

T. Jomori ◽

S. Natori

Keyword(s):

Molecular Cloning ◽

Acute Phase ◽

Periplaneta Americana ◽

Binding Protein ◽

American Cockroach ◽

Lipopolysaccharide Binding Protein ◽

Lipopolysaccharide Binding

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Preparation of Antioxidant and Antibacterial Chitosan Film from Periplaneta americana

Insects ◽

10.3390/insects12010053 ◽

2021 ◽

Vol 12 (1) ◽

pp. 53

Author(s):

Sicong Chen ◽

Xunfan Wei ◽

Zhuoxiao Sui ◽

Mengyuan Guo ◽

Jin Geng ◽

...

Keyword(s):

Food Packaging ◽

Periplaneta Americana ◽

Uv Light ◽

Radical Scavenging ◽

Chitosan Film ◽

American Cockroach ◽

Raw Material ◽

Potential Resource ◽

Radical Scavenging Ability ◽

Dpph Radical Scavenging

Among different insects, the American cockroach (Periplaneta americana) has been bred in industrial scale successfully as a potential resource of protein, lipid, and antibacterial peptide. However, the application of its chitosan has not been studied widely, which has hindered the sufficient utilization of P. americana. In this paper, the chitosan from P. americana was separated, characterized, and processed into film (PaCSF) to examine its potential of being applied in food packaging. As the results of different characterizations showed, PaCSF was similar to shrimp chitosan film (SCSF). However, concerning the performances relating to food packaging, the two chitosan films were different. PaCSF contained more water (42.82%) than SCSF did, resulting in its larger thickness (0.08 mm). PaCSF could resist UV light more effectively than SCSF did. Concerning antioxidant activity, the DPPH radical scavenging ability of PaCSF increased linearly with time passing, reaching 72.46% after 8 h, which was better than that of SCSF. The antibacterial activity assay exhibited that PaCSF resisted the growth of Serratia marcescens and Escherichia coli more effectively than SCSF did. The results implied that P. americana chitosan could be a potential raw material for food packaging, providing a new way to develop P. americana.

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