scholarly journals A Novel Terrestrial Rabies Virus Lineage Occurring in South America: Origin, Diversification, and Evidence of Contact between Wild and Domestic Cycles

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2484
Author(s):  
Diego A. Caraballo ◽  
Cristina Lema ◽  
Laura Novaro ◽  
Federico Gury-Dohmen ◽  
Susana Russo ◽  
...  
Keyword(s):  
South America ◽  
Rabies Virus ◽  
Purifying Selection ◽  
Host Shift ◽  
Domestic Species ◽  
Nucleoprotein Gene ◽  
Consensus Sequences ◽  
Host Shifts ◽  
Single Host ◽  
Related Variant

The rabies virus (RABV) is characterized by a history dominated by host shifts within and among bats and carnivores. One of the main outcomes of long-term RABV maintenance in dogs was the establishment of variants in a wide variety of mesocarnivores. In this study, we present the most comprehensive phylogenetic and phylogeographic analysis, contributing to a better understanding of the origins, diversification, and the role of different host species in the evolution and diffusion of a dog-related variant endemic of South America. A total of 237 complete Nucleoprotein gene sequences were studied, corresponding to wild and domestic species, performing selection analyses, ancestral states reconstructions, and recombination analyses. This variant originated in Brazil and disseminated through Argentina and Paraguay, where a previously unknown lineage was found. A single host shift was identified in the phylogeny, from dog to the crab-eating fox (Cerdocyon thous) in the Northeast of Brazil. Although this process occurred in a background of purifying selection, there is evidence of adaptive evolution -or selection of sub-consensus sequences- in internal branches after the host shift. The interaction of domestic and wild cycles persisted after host switching, as revealed by spillover and putative recombination events.

scholarly journals Dynamics of viral index infections in novel hosts

2020 ◽  
Author(s):  
Nardus Mollentze ◽  
Daniel G. Streicker ◽  
Pablo R. Murcia ◽  
Katie Hampson ◽  
Roman Biek
Keyword(s):  
Rabies Virus ◽  
Host Species ◽  
Host Shift ◽  
Single Infection ◽  
Limiting Factor ◽  
New Host ◽  
Host Shifts ◽  
Virus Excretion ◽  
Novel Host ◽  
Meta Analyses

AbstractWhether a pathogen entering a new host species results in a single infection or in onward transmission, and potentially an outbreak, depends upon the progression of infection in the index case. Although index infections are rarely observable in nature, experimental inoculations of pathogens into novel host species have a long history in biomedical research. This provides a rich and largely unexploited data source for meta-analyses to identify the host and pathogen determinants of variability in infection outcomes. Here, we analysed the progressions of 514 experimental cross-species inoculations of rabies virus, a widespread zoonotic pathogen which in nature exhibits both dead end infections and varying levels of sustained transmission in novel hosts. Inoculations originating from bats rather than carnivores, and from warmer to cooler-bodied species caused infections with shorter incubation periods that were associated with diminished virus excretion. Inoculations between distantly related hosts tended to result in shorter clinical disease periods, which will also impede transmission. All effects were modulated by infection dose and together suggest that increased virulence as host species become more dissimilar is the limiting factor preventing onward transmission. These results explain observed constraints on rabies virus host shifts, allow us to evaluate the risk of novel reservoirs establishing, and give mechanistic insights into why host shifts are less likely between genetically distant species. More generally, our study highlights meta-analyses of experimental infections as a tractable approach to quantify the complex interactions between virus, reservoir, and novel host that shape the outcome of cross-species transmission.Significance statementEmerging disease epidemics often result from a pathogen establishing transmission in a novel host species. However, most cross-species transmissions fail to establish in the newly infected species for reasons that remain poorly understood. Examining cross-species inoculations involving rabies, a widespread viral zoonosis, we show that mismatches in virulence, which are predictable from host and viral factors, make sustained transmission in the novel host less likely. In particular, disease progression was accelerated and virus excretion decreased when the reservoir and novel host were physiologically or genetically more dissimilar. These mechanistic insights help to explain and predict host shift events and highlight meta-analyses of existing experimental inoculation data as a powerful and generalisable approach for understanding the dynamics of index infections in novel species.

scholarly journals Dynamics of rabies virus quasispecies during serial passages in heterologous hosts

1999 ◽  
Vol 80 (8) ◽  
pp. 2041-2050 ◽  
Author(s):  
B. Kissi ◽  
H. Badrane ◽  
L. Audry ◽  
A. Lavenu ◽  
N. Tordo ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Environmental Changes ◽  
Virus Genome ◽  
Genomic Region ◽  
Virus Infections ◽  
Nucleoprotein Gene ◽  
Consensus Sequences ◽  
Glycoprotein Gene ◽  
New Hosts ◽  
The Stability

To understand the mutations and genetic rearrangements that allow rabies virus infections of new hosts and adaptation in nature, the quasispecies structure of the nucleoprotein and glycoprotein genes as well as two noncoding sequences of a rabies virus genome were determined. Gene sequences were obtained from the brain and from the salivary glands of the original host, a naturally infected European fox, and after serial passages in mice, dogs, cats and cell culture. A relative genetic stasis of the consensus sequences confirmed previous results about the stability of rabies virus. At the quasispecies level, the mutation frequency varies, in the following order: glycoprotein region (21·9×10−4 mutations per bp), noncoding sequence nucleoprotein–phosphoprotein region (7·2–7·9×10−4 mutations per bp) and nucleoprotein gene region (2·9–3·7×10−4 mutations per bp). These frequencies varied according to the number, type of heterologous passages and the genomic region considered. The shape of the quasispecies structure was dramatically modified by passages in mice, in which the mutation frequencies increased by 12–31×10−4 mutations per bp, depending on the region considered. Nonsynonymous mutations were preponderant particularly in the glycoprotein gene, stressing the importance of positive selection in the maintenance and fixation of substitutions. Two mechanisms of genomic evolution of the rabies virus quasispecies, while adapting to environmental changes, have been identified: a limited accumulation of mutations with no replacement of the original master sequence and a less frequent but rapid selective overgrowth of favoured variants.

scholarly journals Genetic analysis of a rabies virus host shift event reveals within-host viral dynamics in a new host

2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Denise A Marston ◽  
Daniel L Horton ◽  
Javier Nunez ◽  
Richard J Ellis ◽  
Richard J Orton ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Rabies Virus ◽  
Host Shift ◽  
Viral Dynamics ◽  
New Host

scholarly journals Rapid divergence in independent aspects of the compatibility phenotype in the Spiroplasma/Drosophila interaction

2021 ◽  
Author(s):  
Joanne S. Griffin ◽  
Michael Gerth ◽  
Gregory D. D. Hurst
Keyword(s):  
Vertical Transmission ◽  
Host Species ◽  
High Efficiency ◽  
Host Shift ◽  
Direct Selection ◽  
New Host ◽  
Host Shifts ◽  
Species Pairs ◽  
Novel Host ◽  
Rapid Divergence

AbstractHeritable symbionts represent important components of host biology, both as antagonistic reproductive parasites and as beneficial protective partners. An important component of heritable microbes’ biology is their ability to establish in new host species, a process equivalent to a host shift for an infectiously transmitted parasite or pathogen. For a host shift to occur, the symbiont must be compatible with the host: it must not cause excess pathology, must have good vertical transmission, and possess a drive phenotype that enables spread. Classically, compatibility has been considered a declining function of genetic distance between novel and ancestral host species. Here we investigate the evolutionary lability of compatibility to heritable microbes by comparing the capacity for a symbiont to establish in two novel host species equally related to the ancestral host. Compatibility of the protective Spiroplasma from D. hydei with D. simulans and D. melanogaster was tested. The Spiroplasma had contrasting compatibility in these two host species. The transinfection showed pathology and low vertical transmission in D. melanogaster but was asymptomatic and transmitted with high efficiency in D. simulans. These results were not affected by the presence/absence of Wolbachia in either of the two species. The pattern of protection was not congruent with that for pathology/transmission, with protection being weaker in the D. simulans, the host in which Spiroplasma was asymptomatic and transmitted well. Further work indicated pathological interactions occurred in D. sechellia and D. yakuba, indicating that D. simulans was unusual in being able to carry the symbiont without damage. The differing compatibility of the symbiont with these closely related host species emphasises first the rapidity with which host-symbiont compatibility evolves despite compatibility itself not being subject to direct selection, and second the independence of the different components of compatibility (pathology, transmission, protection). This requirement to fit three different independently evolving aspects of compatibility, if commonly observed, is likely to be a major feature limiting the rate of host shifts. Moving forward, the variation between sibling species pairs observed above provides an opportunity to identify the mechanisms behind variable compatibility between closely related host species, which will drive hypotheses as to the evolutionary drivers of compatibility variation.

scholarly journals Correction to: Sequence analysis of nucleoprotein gene reveals the co-circulation of lineages and sublineages of rabies virus in herbivorous in Rio Grande do Sul state, Brazil

2020 ◽  
Vol 51 (2) ◽  
pp. 849-850
Author(s):  
Gisane L. de Almeida ◽  
Juliana F. Cargnelutti ◽  
Ananda S. Ries ◽  
José C. Ferreira ◽  
Júlio C. A. Rosa ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Rabies Virus ◽  
Rio Grande ◽  
Rio Grande Do Sul ◽  
Nucleoprotein Gene

Typing of the rabies virus in Chile, 2002–2008

2012 ◽  
Vol 140 (12) ◽  
pp. 2157-2162 ◽  
Author(s):  
V. YUNG ◽  
M. FAVI ◽  
J. FERNANDEZ
Keyword(s):  
Phylogenetic Analysis ◽  
Monoclonal Antibodies ◽  
Rabies Virus ◽  
Tadarida Brasiliensis ◽  
Insectivorous Bats ◽  
Nucleoprotein Gene ◽  
Nucleoprotein N

SUMMARYIn Chile, dog rabies has been controlled and insectivorous bats have been identified as the main rabies reservoir. This study aimed to determine the rabies virus (RABV) variants circulating in the country between 2002 and 2008. A total of 612 RABV isolates were tested using a panel with eight monoclonal antibodies against the viral nucleoprotein (N-mAbs) for antigenic typing, and a product of 320-bp of the nucleoprotein gene was sequenced from 99 isolates. Typing of the isolates revealed six different antigenic variants but phylogenetic analysis identified four clusters associated with four different bat species. Tadarida brasiliensis bats were confirmed as the main reservoir. This methodology identified several independent rabies enzootics maintained by different species of insectivorous bats in Chile.

scholarly journals A permanent host shift of rabies virus from Chiroptera to Carnivora associated with recombination

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Nai-Zheng Ding ◽  
Dong-Shuai Xu ◽  
Yuan-Yuan Sun ◽  
Hong-Bin He ◽  
Cheng-Qiang He
Keyword(s):  
Rabies Virus ◽  
Host Shift

scholarly journals Divergence before the host shift? Prezygotic reproductive isolation among three varieties of a specialist fly on a single host plant

2016 ◽  
Vol 41 (4) ◽  
pp. 389-399 ◽  
Author(s):  
ALAINE C. HIPPEE ◽  
MAREN E. ELNES ◽  
JAROD S. ARMENTA ◽  
MARTY A. CONDON ◽  
ANDREW A. FORBES
Keyword(s):  
Reproductive Isolation ◽  
Host Plant ◽  
Host Shift ◽  
Single Host

scholarly journals Ancient DNA reveals the lost domestication history of South American camelids in Northern Chile and across the Andes

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Paloma Diaz-Maroto ◽  
Alba Rey-Iglesia ◽  
Isabel Cartajena ◽  
Lautaro Núñez ◽  
Michael V Westbury ◽  
...  
Keyword(s):  
South America ◽  
Formative Period ◽  
Archaeological Sites ◽  
South American ◽  
Northern Chile ◽  
Spanish Conquest ◽  
Domestic Species ◽  
South American Camelids ◽  
History Of ◽  
Early Formative

The study of South American camelids and their domestication is a highly debated topic in zooarchaeology. Identifying the domestic species (alpaca and llama) in archaeological sites based solely on morphological data is challenging due to their similarity with respect to their wild ancestors. Using genetic methods also presents challenges due to the hybridization history of the domestic species, which are thought to have extensively hybridized following the Spanish conquest of South America that resulted in camelids slaughtered en masse. In this study, we generated mitochondrial genomes for 61 ancient South American camelids dated between 3,500 and 2,400 years before the present (Early Formative period) from two archaeological sites in Northern Chile (Tulán-54 and Tulán-85), as well as 66 modern camelid mitogenomes and 815 modern mitochondrial control region sequences from across South America. In addition, we performed osteometric analyses to differentiate big and small body size camelids. A comparative analysis of these data suggests that a substantial proportion of the ancient vicuña genetic variation has been lost since the Early Formative period, as it is not present in modern specimens. Moreover, we propose a domestication hypothesis that includes an ancient guanaco population that no longer exists. Finally, we find evidence that interbreeding practices were widespread during the domestication process by the early camelid herders in the Atacama during the Early Formative period and predating the Spanish conquest.

Sign in / Sign up

Export Citation Format

Share Document