scholarly journals Synthetic Peptides Containing Three Neutralizing Epitopes of Genotype 4 Swine Hepatitis E Virus ORF2 induced Protection against Swine HEV Infection in Rabbit

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 178
Author(s):  
Yiyang Chen ◽  
Tianxiang Chen ◽  
Yuhang Luo ◽  
Jie Fan ◽  
Meimei Zhang ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Keyhole Limpet Hemocyanin ◽  
Hepatitis E ◽  
Genotype 4 ◽  
Virus Shedding ◽  
Fecal Shedding ◽  
Orf2 Protein ◽  
Swine Hepatitis ◽  
Phosphate Buffered Saline ◽  
Neutralizing Epitopes

Genotype 4 hepatitis E virus (HEV) is a zoonotic pathogen transmitted to humans through food and water. Previously, three genotype 4 swine HEV ORF2 peptides (407EPTV410, 410VKLYTS415, and 458PSRPF462) were identified as epitopes of virus-neutralizing monoclonal antibodies that partially blocked rabbit infection with swine HEV. Here, individual and tandem fused peptides were synthesized, conjugated to keyhole limpet hemocyanin (KLH), then evaluated for immunoprotection of rabbits against swine HEV infection. Forty New Zealand White rabbits were randomly assigned to eight groups; groups 1 thru 5 received three immunizations with EPTV-KLH, VKLYTS-KLH, PSRPF-KLH, EPTVKLYTS-KLH, or EPTVKLYTSPSRPF-KLH, respectively; group 6 received truncated swine HEV ORF2 protein (sp239), and group 7 received phosphate-buffered saline. After an intravenous swine HEV challenge, all group 7 rabbits exhibited viremia and fecal virus shedding by 2–4 weeks post challenge (wpc), seroconversion by 4–9 wpc, elevated alanine aminotransferase (ALT) at 2 wpc, and severe liver lymphocytic venous periphlebitis. Only 1–2 rabbits/group in groups 1–4 exhibited delayed viremia, fecal shedding, seroconversion, increased ALT levels, and slight liver lymphocytic venous periphlebitis; groups 5–6 showed no pathogenic effects. Collectively, these results demonstrate that immunization with a polypeptide containing three genotype 4 HEV ORF2 neutralizing epitopes completely protected rabbits against swine HEV infection.

Experimental infection of mongolian gerbils by a genotype 4 strain of swine hepatitis E virus

2009 ◽  
Vol 81 (9) ◽  
pp. 1591-1596 ◽  
Author(s):  
Wengui Li ◽  
Quan Sun ◽  
Ruiping She ◽  
Decheng Wang ◽  
Xinhui Duan ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Mongolian Gerbils ◽  
Genotype 4 ◽  
Swine Hepatitis

Characterization of antigenic epitopes of the ORF2 protein from hepatitis E virus genotype 4

2009 ◽  
Vol 142 (1-2) ◽  
pp. 140-143 ◽  
Author(s):  
Hongmei Zhang ◽  
Xing Dai ◽  
Xiangnian Shan ◽  
Jihong Meng
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Virus Genotype ◽  
Genotype 4 ◽  
Orf2 Protein ◽  
Antigenic Epitopes

scholarly journals Capped RNA Transcripts of Full-Length cDNA Clones of Swine Hepatitis E Virus Are Replication Competent When Transfected into Huh7 Cells and Infectious When Intrahepatically Inoculated into Pigs

2005 ◽  
Vol 79 (3) ◽  
pp. 1552-1558 ◽  
Author(s):  
Y. W. Huang ◽  
G. Haqshenas ◽  
C. Kasorndorkbua ◽  
P. G. Halbur ◽  
S. U. Emerson ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Cdna Clones ◽  
Virus Shedding ◽  
Full Length Cdna ◽  
Rna Transcripts ◽  
Huh7 Cells ◽  
Swine Hepatitis ◽  
Fecal Virus

ABSTRACT Swine hepatitis E virus (swine HEV), the first animal strain of HEV to be isolated, is a zoonotic agent. We report here the construction and in vitro and in vivo characterizations of infectious cDNA clones of swine HEV. Eight overlapping fragments spanning the entire genome were amplified by reverse transcription-PCR and assembled into a full-length cDNA clone, clone C, which contained 14 mutations compared to the consensus sequence of swine HEV. RNA transcripts from clone C were not infectious, as determined by intrahepatic inoculation into pigs and by in vitro transfection of Huh7 cells. Multiple site-based site-directed mutagenesis was performed to generate three new cDNA clones (pSHEV-1, pSHEV-2, and pSHEV-3) which differed from each other. The transfection of capped RNA transcripts into human liver Huh7 cells resulted in the synthesis of both ORF2 capsid and ORF3 proteins, indicating that the cDNA clones were replication competent. Each of the three clones resulted in active swine HEV infections after the intrahepatic inoculation of pigs with capped RNA transcripts. The patterns of seroconversion, viremia, and fecal virus shedding for pigs inoculated with RNA transcripts from clones pSHEV-2 and pSHEV-3 were similar to each other and to those for pigs inoculated with wild-type swine HEV, suggesting that the nucleotide differences between these two cDNA clones were not critical for replication. Pigs inoculated with RNA transcripts from clone pSHEV-1, which contained three nonsilent mutations in the ORF2 capsid gene, had a delayed appearance of seroconversion and fecal virus shedding and had undetectable viremia. The availability of these infectious cDNA clones affords us an opportunity to understand the mechanisms of cross-species infection by constructing chimeric human and swine HEVs.

scholarly journals Detection of Genotype 4 Swine Hepatitis E Virus in Systemic Tissues in Cross-Species Infected Rabbits

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0171277 ◽  
Author(s):  
Qiaoxing Wu ◽  
Junqing An ◽  
Ruiping She ◽  
Ruihan Shi ◽  
Wenzhuo Hao ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Genotype 4 ◽  
Swine Hepatitis

Immunogenicity and protective efficacy in rhesus monkeys of a recombinant ORF2 protein from hepatitis E virus genotype 4

2009 ◽  
Vol 154 (3) ◽  
pp. 481-488 ◽  
Author(s):  
W. J. Huang ◽  
H. Y. Zhang ◽  
T. J. Harrison ◽  
H. Y. Lan ◽  
G. Y. Huang ◽  
...  
Keyword(s):  
Rhesus Monkeys ◽  
Hepatitis E Virus ◽  
Protective Efficacy ◽  
Hepatitis E ◽  
Virus Genotype ◽  
Genotype 4 ◽  
Recombinant Orf2 Protein ◽  
Orf2 Protein

scholarly journals Determination of Fecal Shedding Rates and Genotypes of Swine Hepatitis E Virus (HEV) in Korea

2008 ◽  
Vol 70 (12) ◽  
pp. 1367-1371 ◽  
Author(s):  
Sung-Eun KIM ◽  
Mi-Young KIM ◽  
Do-Geun KIM ◽  
Young-Jo SONG ◽  
Hyun-Jeong JEONG ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Fecal Shedding ◽  
Swine Hepatitis

scholarly journals Seasonal pattern of hepatitis E virus prevalence in swine in two different geographical areas of China

2013 ◽  
Vol 141 (11) ◽  
pp. 2403-2409 ◽  
Author(s):  
Y. H. LU ◽  
H. Z. QIAN ◽  
A. Q. HU ◽  
X. QIN ◽  
Q. W. JIANG ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Seasonal Pattern ◽  
Eastern China ◽  
Geographical Area ◽  
Hepatitis E ◽  
Southwestern China ◽  
Seasonal Patterns ◽  
Virus Prevalence ◽  
Swine Hepatitis ◽  
A Minor

SUMMARYWe studied seasonal patterns of swine hepatitis E virus (HEV) infection in China. From 2008 to 2011, 4200 swine bile specimens were collected for the detection of HEV RNA. A total of 92/2400 (3·83%) specimens in eastern China and 47/1800 (2·61%) specimens in southwestern China were positive for HEV. Seasonal patterns differing by geographical area were suggested. In eastern China, the major peak of HEV RNA prevalence was during March–April, with a minor peak during September–October, and a dip during July–August. In southwestern China, the peak was during September–October and the dip during March–April. The majority of subtype 4a cases (63/82, 76·83%) were detected in the first half of the year, while the majority of subtype 4b cases (26/29, 89·66%) were concentrated in the second half of the year, suggesting that different subtypes contribute to different peaks. Our results indicate that the distribution of HEV subtypes is associated with seasonal patterns.

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