scholarly journals Microbial Biofilm Diversity and Prevalence of Antibiotic Resistance Genes in Drinking Water Distribution System of Peshawar, Pakistan

Water ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 1788
Author(s):  
Habib Ullah ◽  
Muhammad Shahzad ◽  
Faizan Saleem ◽  
Taj Ali ◽  
Muhammad Kamran Azim ◽  
...  

The occurrence of microbial communities harboring antibiotic resistance bacteria and antibiotic resistance genes in the drinking water distribution system pose a significant threat to the aquatic ecosystem and to public health, especially in developing countries. In this study, we have used next-generation sequencing technology to explore bacterial community diversity and the abundance of antibiotic resistance genes in biofilms collected from the drinking water distribution system of Peshawar, the capital city of the Khyber Pakhtunkhwa province of Pakistan. The results showed that Proteobacteria were the most abundant phyla (89.79%) in all biofilm samples, followed by Bacteroidetes (3.48%) and Actinobacteria (2.79%). At genus level, Pseudomonas was the most common (22.45%) in all biofilm samples. Overall, bacterial diversity and richness was higher in biofilm samples collected from the consumer end than the source site. Bacterial diversity was also dependent on the piping material (GI vs. PVC) and water supply (direct vs. indirect). Functional annotation reveals a differential abundance of common metabolic pathways at source and consumer end. Resistome analysis revealed a prevalence of resistance genes against 12 classes of antibiotics in all samples with macrolides resistance being the commonest at the consumer end (42.1%) and fluoroquinolone resistance at the source end (24%). To our knowledge, this is the first study that provides new insight and evidence into the microbial community diversity and antibiotic resistance in the drinking water supply system of Peshawar. These findings may ultimately help the authorities to design and implement effective strategies for controlling biofilms and ensuring a continuous supply of safe drinking water to the community.

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Vicente Gomez-Alvarez ◽  
Stacy Pfaller ◽  
Randy P. Revetta

We report the draft genomes of two Sphingopyxis sp. strains isolated from a chloraminated drinking water distribution system simulator. Both strains are ubiquitous residents and early colonizers of water distribution systems. Genomic annotation identified a class 1 integron ( intI1 ) gene associated with sulfonamide ( sul1 ) and puromycin ( pac ) antibiotic resistance genes.


2010 ◽  
Vol 8 (3) ◽  
pp. 593-600 ◽  
Author(s):  
Xiaohui Bai ◽  
Fan Wu ◽  
Binhui Zhou ◽  
Xinghua Zhi

Community diversity and abundance of biofilms from a full-scale drinking water distribution system in Shanghai were characterized by denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA sequences and heterotrophic plate count (HPC), respectively. Bacteria affiliated to the Beta- and Gamma-Proteobacteria were dominating in both in-situ and HPC-culturable bacterial communities. Other bacteria present included members of Alphaproteobacteria, Bacteroides, Actinobacteria, Nitrospirae and Firmicutes. Acidovorax, Ralstonia and Acinetobacter were common species in biofilms. Klebsiella pneumoniae and Enterobacter sp. were detected in the local distribution system. Dissolved organic carbon (DOC), residual disinfectant and temperature were the most important factors influencing both bacterial abundance and composition. HPC for biofilm sample was not correlated with its community diversity.


Water ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2601
Author(s):  
Agata Siedlecka ◽  
Mirela Wolf-Baca ◽  
Katarzyna Piekarska

Antibiotic resistance of bacteria is an emerging problem in drinking water treatment. This paper presents the comparison of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) prevalence during the summer and winter season in a full-scale drinking water distribution system (DWDS) supplied by two water treatment plants (WTPs). The effect of distance from WTP and physical–chemical water parameters on its microbial properties was also tested. Bacterial consortia dwelling in bulk tap water were additionally compared by means of denaturating gradient gel electrophoresis (DGGE). The results showed that among ARB, bacteria resistant to ceftazidime (CAZ) were the most abundant, followed by bacteria resistant to amoxicillin (AML), ciprofloxacin (CIP), and tetracycline (TE). Numerous ARGs were detected in tested tap water samples. Only CAZ resistant bacteria were more prevalent in the season of increased antibiotic consumption, and only AML resistant bacteria relative abundances increase was statistically significant with the distance from a WTP. The investigated tap water meets all legal requirements. It is therefore safe to drink according to the law. Nevertheless, because antibiotic resistance could pose a threat to consumer health, it should be further monitored in DWDSs.


2009 ◽  
Vol 43 (20) ◽  
pp. 5005-5014 ◽  
Author(s):  
Jeffrey G. Szabo ◽  
Christopher A. Impellitteri ◽  
Shekar Govindaswamy ◽  
John S. Hall

2007 ◽  
Vol 2007 (1) ◽  
pp. 449-467
Author(s):  
Stacia L. Thompson ◽  
Elizabeth Casman ◽  
Paul Fischbeck ◽  
Mitchell J. Small ◽  
Jeanne M. VanBriesen

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