scholarly journals EXPERT POSSIBILITIES OF FORENSIC CYTOLOGY INVESTIGATION IN FORENSIC EXAMINATION OF MATERIAL EVIDENCE

2020 ◽  
pp. 738-744
Author(s):  
R. Starovoitova ◽  
T. Lichman

The article presents the history of the development and implementation of forensic cytological research methods in the practice of conducting forensic examinations of material evidence in the forensic examination bureau of Ukraine. The modern possibilities of forensic cytology methods for solving investigative issues in solving crimes against human life and health are shown in the article. Due to the fact, that the object of study of expert cytologists and expert geneticists is the same substance, namely, nuclear cells of different tissues and organs, blood leukocytes, epithelial cells of saliva, vagina, rectum, sperm, and cells of the outer root vagina hair – forensic cytological studies necessitate the preservation of nuclear cells for further genetic analysis. To this end, the staff of the Forensic Cytology Department of the Main Bureau of Forensic Examination developed a method for the preparation of cytological preparations using an ultrasonic water bath, which allows increasing the number of cellular elements extracted. At the same time, the specialists of the Odesa Regional Bureau of Forensic Examination introduced a technique for conducting molecular genetic analysis of nucleated cells in cytological preparations. Such techniques allow, even with a very small amount of biomaterial, to answer most of the questions posed by the investigator to the expert.

2021 ◽  
Vol 16 (1) ◽  
pp. 6-18
Author(s):  
S. A. Smirnova ◽  
G. G. Omel’yanyuk ◽  
I. V. Storozhenko ◽  
A. A. Rybakova ◽  
V. V. Gulevskaya

The article addresses the importance and basic preconditions for the forming a new direction of forensic activity in the system of forensic institutions of the Russian Ministry of Justice a new direction of forensic activity - molecular-genetic analysis of the objects of biological origin. The authors present the advantages of DNA analysis - one of the most modern and efficient methods in investigating criminal cases. The article also demonstrates the potential of different methods of DNA-analysis for forensic investigations. The history of forensic DNA-analysis development in Russia and its features when examining the human, animal, and plant biomaterials are briefly discussed. The authors propose the definitions for the molecular-genetic examinations’ object and subject, formulate the model tasks, and suggest a list of sample questions for this study.


2017 ◽  
pp. 110-114
Author(s):  
A. G. Skuratov ◽  
A. N. Lyzikov ◽  
E. V. Voropayev ◽  
O. V. Osipkina

Objective: to assess the association of polymorphism of the markers of interleukin-4 (IL-4) and tumor necrosis factor (TNF-α) in patients with liver cirrhosis with severe pathological process. Material and methods. The research material was DNA isolated from blood leukocytes of the patients. To identify SNP data, the PCR method was chosen with subsequent restriction analysis and electrophoretic detection by horizontal gel electrophoresis and amplification with visualization of the obtained results. Results. The performed molecular and genetic analysis of IL-4 and TNF-α gene polymorphisms made it possible to assume a relation between the frequency of polymorphic genotypes and alleles of the investigated genes and the severity of liver cirrhosis and portal hypertension, which can be used to diagnose and predict the severe course of the disease.


2016 ◽  
Vol 2 (3) ◽  
pp. 261-264 ◽  
Author(s):  
Anders Krogh Broendberg ◽  
Lisbeth Noerum Pedersen ◽  
Jens Cosedis Nielsen ◽  
Henrik Kjaerulf Jensen

1992 ◽  
Vol 174 (20) ◽  
pp. 6404-6410 ◽  
Author(s):  
R J Limberger ◽  
L L Slivienski ◽  
D B Yelton ◽  
N W Charon

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